Literature DB >> 19924478

Yeast microflora isolated from brazilian cassava roots: taxonomical classification based on molecular identification.

Nelson Ferreira1, Carmela Belloch, Amparo Querol, Paloma Manzanares, Salvador Vallez, Alberdan Santos.   

Abstract

A rapid molecular identification technique was applied on microbial microflora isolated from Brazilian cassava roots given a yeast profile presented in the samples analyzed. A total of 24 strain isolated from cassava were initially grouped and identified in five groups using restriction-fragment length polymorphism (RFLPs) of 5.8S-ITS rDNA region. Sequencing analysis of the domains D1 and D2 of the 26S rRNA gene or 5.8S rRNA-ITS region were used to identify different groups of yeasts. Representative colonies of yeasts of each group were isolated and identified as Debaromyces hansenii, Kodamaea ohmeri, Candida glabrata, C. haemulonii, and Pichia gullhermondii. It is hoped that these results will contribute toward selecting yeast from this microflora capable to degrade cassava starch in the near future.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19924478     DOI： 10.1007/s00284-009-9539-z

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  9 in total

1.  Analysis of fungal diversity in the wheat rhizosphere by sequencing of cloned PCR-amplified genes encoding 18S rRNA and temperature gradient gel electrophoresis.

Authors:  E Smit; P Leeflang; B Glandorf; J D van Elsas; K Wernars
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

2.  MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0.

Authors:  Koichiro Tamura; Joel Dudley; Masatoshi Nei; Sudhir Kumar
Journal:  Mol Biol Evol       Date:  2007-05-07       Impact factor: 16.240

3.  Comparison of six DNA extraction methods for recovery of fungal DNA as assessed by quantitative PCR.

Authors:  David N Fredricks; Caitlin Smith; Amalia Meier
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

4.  Molecular monitoring of wine fermentations conducted by active dry yeast strains.

Authors:  A Querol; E Barrio; T Huerta; D Ramón
Journal:  Appl Environ Microbiol       Date:  1992-09       Impact factor: 4.792

5.  Identification of yeasts by RFLP analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers.

Authors:  B Esteve-Zarzoso; C Belloch; F Uruburu; A Querol
Journal:  Int J Syst Bacteriol       Date:  1999-01

6.  Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5' end of the large-subunit (26S) ribosomal DNA gene.

Authors:  C P Kurtzman; C J Robnett
Journal:  J Clin Microbiol       Date:  1997-05       Impact factor: 5.948

7.  The phylogeny of Yamadazyma ohmeri (Etchells et Bell) Billon-Grand based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Kodamaea gen. nov. (Saccharomycetaceae).

Authors:  Y Yamada; T Suzuki; M Matsuda; K Mikata
Journal:  Biosci Biotechnol Biochem       Date:  1995-06       Impact factor: 2.043

8.  Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences.

Authors:  C P Kurtzman; C J Robnett
Journal:  Antonie Van Leeuwenhoek       Date:  1998-05       Impact factor: 2.271

9.  Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions.

Authors:  Shiang Ning Leaw; Hsien Chang Chang; Hsiao Fang Sun; Richard Barton; Jean-Philippe Bouchara; Tsung Chain Chang
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948
  9 in total
  4 in total

1.  Identification of predominant yeasts associated with artisan Mexican cocoa fermentations using culture-dependent and culture-independent approaches.

Authors:  A Arana-Sánchez; L E Segura-García; M Kirchmayr; I Orozco-Ávila; E Lugo-Cervantes; A Gschaedler-Mathis
Journal:  World J Microbiol Biotechnol       Date:  2015-01-08       Impact factor: 3.312

2.  Assessment of Multi Fragment Melting Analysis System (MFMAS) for the Identification of Food-Borne Yeasts.

Authors:  Zülal Kesmen; Mine E Büyükkiraz; Esra Özbekar; Mete Çelik; F Özge Özkök; Özge Kılıç; Bülent Çetin; Hasan Yetim
Journal:  Curr Microbiol       Date:  2018-02-26       Impact factor: 2.188

3.  A survey of the mycobiota associated with larvae of the black soldier fly (Hermetia illucens) reared for feed production.

Authors:  Ilaria Varotto Boccazzi; Matteo Ottoboni; Elena Martin; Francesco Comandatore; Lisa Vallone; Thomas Spranghers; Mia Eeckhout; Valeria Mereghetti; Luciano Pinotti; Sara Epis
Journal:  PLoS One       Date:  2017-08-03       Impact factor: 3.240

4.  On the Origins of a Species: What Might Explain the Rise of Candida auris?

Authors:  Brendan R Jackson; Nancy Chow; Kaitlin Forsberg; Anastasia P Litvintseva; Shawn R Lockhart; Rory Welsh; Snigdha Vallabhaneni; Tom Chiller
Journal:  J Fungi (Basel)       Date:  2019-07-06
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.