| Literature DB >> 19923743 |
Keisuke Sugimoto1, Yoshihiro Yamamoto, Siswanto Antoni, Miki Senda, Daisuke Kasai, Eiji Masai, Masao Fukuda, Toshiya Senda.
Abstract
Gallate dioxygenase (DesB) from Sphingobium sp. SYK-6, which belongs to the type II extradiol dioxygenase family, was purified and crystallized using the hanging-drop vapour-diffusion method. Two crystal forms were obtained. The form I crystal belonged to space group C2, with unit-cell parameters a = 136.2, b = 53.6, c = 55.1 angstrom, beta = 112.8 degrees, and diffracted to 1.6 angstrom resolution. The form II crystal belonged to space group P2(1), with unit-cell parameters a = 56.2, b = 64.7, c = 116.1 angstrom, beta = 95.1 degrees, and diffracted to 1.9 angstrom resolution. A molecular-replacement calculation using LigAB as a search model yielded a satisfactory solution for both crystal forms.Entities:
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Year: 2009 PMID： 19923743 PMCID： PMC2777051 DOI： 10.1107/S1744309109041086
Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091