AIM OF THE STUDY: The genotoxic and antigenotoxic potential of the ethanolic extract of Hemidesmus indicus roots were evaluated in cultured human lymphocytes using cisplatin as the positive mutagen. MATERIALS AND METHODS: Cytogenetic damage and cytotoxicity were determined in cells exposed to different doses of the extract, ranging from 2 to 32 microg/ml of culture medium, either alone or together with cisplatin. RESULTS: There was a significant reduction in cisplatin-induced frequencies of sister chromatid exchanges, chromosome aberrations and micronucleated binucleate cells at the lower concentrations of 4 and 8 microg/ml (P<0.05). However, the extract by itself reduced the proliferative rate index, mitotic index and cytokinesis-block proliferative index (P<0.05). Further, a significant increase in the percentage of chromosome aberrations was noticed at the higher concentrations. CONCLUSION: Hemidesmus indicus root extract possesses significant genoprotective effect at the lower concentrations although it is cytotoxic and probably genotoxic at higher doses. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
AIM OF THE STUDY: The genotoxic and antigenotoxic potential of the ethanolic extract of Hemidesmus indicus roots were evaluated in cultured human lymphocytes using cisplatin as the positive mutagen. MATERIALS AND METHODS: Cytogenetic damage and cytotoxicity were determined in cells exposed to different doses of the extract, ranging from 2 to 32 microg/ml of culture medium, either alone or together with cisplatin. RESULTS: There was a significant reduction in cisplatin-induced frequencies of sister chromatid exchanges, chromosome aberrations and micronucleated binucleate cells at the lower concentrations of 4 and 8 microg/ml (P<0.05). However, the extract by itself reduced the proliferative rate index, mitotic index and cytokinesis-block proliferative index (P<0.05). Further, a significant increase in the percentage of chromosome aberrations was noticed at the higher concentrations. CONCLUSION:Hemidesmus indicus root extract possesses significant genoprotective effect at the lower concentrations although it is cytotoxic and probably genotoxic at higher doses. Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
Authors: Recep Dursun; Yılmaz Zengin; Ercan Gündüz; Mustafa İçer; Hasan Mansur Durgun; Mansur Dağgulli; İbrahim Kaplan; Ulaş Alabalık; Cahfer Güloğlu Journal: Int J Clin Exp Med Date: 2015-02-15
Authors: Ercan Gündüz; Recep Dursun; Yılmaz Zengin; Mustafa İçer; Hasan Mansur Durgun; Ayşe Kanıcı; İbrahim Kaplan; Ulaş Alabalık; Hüseyin Gürbüz; Cahfer Güloğlu Journal: Int J Clin Exp Med Date: 2015-05-15
Authors: Carlos F Araujo-Lima; Andreia S Fernandes; Erika M Gomes; Larisse L Oliveira; Andrea F Macedo; Rosemar Antoniassi; Allan E Wilhelm; Claudia A F Aiub; Israel Felzenszwalb Journal: Oxid Med Cell Longev Date: 2018-05-02 Impact factor: 6.543