Literature DB >> 19892176

Tagging for protein expression.

Arun Malhotra1.   

Abstract

Tags are frequently used in the expression of recombinant proteins to improve solubility and for affinity purification. A large number of tags have been developed for protein production and researchers face a profusion of choices when designing expression constructs. Here, we survey common affinity and solubility tags, and offer some guidance on their selection and use.

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Year:  2009        PMID： 19892176     DOI： 10.1016/S0076-6879(09)63016-0

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  33 in total

1.  Application of protein engineering to enhance crystallizability and improve crystal properties.

Authors:  Zygmunt S Derewenda
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-04-21

Review 2.  Histidine-tag-directed chromophores for tracer analyses in the analytical ultracentrifuge.

Authors:  Lance M Hellman; Chunxia Zhao; Manana Melikishvili; Xiaorong Tao; James E Hopper; Sidney W Whiteheart; Michael G Fried
Journal:  Methods       Date:  2010-12-25       Impact factor: 3.608

Review 3.  To fuse or not to fuse: what is your purpose?

Authors:  Mark R Bell; Mark J Engleka; Asim Malik; James E Strickler
Journal:  Protein Sci       Date:  2013-09-17       Impact factor: 6.725

Review 4.  Nuclear magnetic resonance spectroscopy of the circadian clock of cyanobacteria.

Authors:  Yong-Gang Chang; Roger Tseng; Nai-Wei Kuo; Andy LiWang
Journal:  Integr Comp Biol       Date:  2013-05-10       Impact factor: 3.326

5.  Effects of the Fc-III tag on activity and stability of green fluorescent protein and human muscle creatine kinase.

Authors:  Shan Feng; Yiyi Gong; Gulishana Adilijiang; Haiteng Deng
Journal:  Protein Sci       Date:  2013-07       Impact factor: 6.725

6.  Protein purification using PDZ affinity chromatography.

Authors:  Ward G Walkup; Mary B Kennedy
Journal:  Curr Protoc Protein Sci       Date:  2015-04-01

7.  Synapsins regulate brain-derived neurotrophic factor-mediated synaptic potentiation and axon elongation by acting on membrane rafts.

Authors:  Hung-Teh Kao; Kanghyun Ryoo; Albert Lin; Stephen R Janoschka; George J Augustine; Barbara Porton
Journal:  Eur J Neurosci       Date:  2017-03-21       Impact factor: 3.386

8.  A Highly Versatile Expression System for the Production of Multiply Phosphorylated Proteins.

Authors:  Phillip Zhu; Philip R Gafken; Ryan A Mehl; Richard B Cooley
Journal:  ACS Chem Biol       Date:  2019-06-17       Impact factor: 5.100

9.  Strep-tag II and Twin-Strep based cassettes for protein tagging by homologous recombination and characterization of endogenous macromolecular assemblies in Saccharomyces cerevisiae.

Authors:  Jay Rai; J Kalyani Pemmasani; Andriy Voronovsky; Ida S Jensen; Arulmani Manavalan; Jens R Nyengaard; Monika M Golas; Bjoern Sander
Journal:  Mol Biotechnol       Date:  2014-11       Impact factor: 2.695

Review 10.  Deep sequencing in library selection projects: what insight does it bring?

Authors:  J Glanville; S D'Angelo; T A Khan; S T Reddy; L Naranjo; F Ferrara; A R M Bradbury
Journal:  Curr Opin Struct Biol       Date:  2015-08       Impact factor: 6.809
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