BACKGROUND AIMS: Effective treatment for stress urinary incontinence (SUI) is lacking. This study investigated whether transplantation of adipose tissue-derived stem cells (ADSC) can treat SUI in a rat model. METHODS: Rats were induced to develop SUI by postpartum vaginal balloon dilation and bilateral ovariectomy. ADSC were isolated from the peri-ovary fat, examined for stem cell properties, and labeled with thymidine analog BrdU or EdU. Ten rats received urethral injection of saline as a control. Twelve rats received urethral injection of EdU-labeled ADSC and six rats received intravenous injection of BrdU-labeled ADSC through the tail vein. Four weeks later, urinary voiding function was assessed by conscious cystometry. The rats were then killed and their urethras harvested for tracking of ADSC and quantification of elastin, collagen and smooth muscle contents. RESULTS: Cystometric analysis showed that eight out 10 rats in the control group had abnormal voiding, whereas four of 12 (33.3%) and two of six (33.3%) rats in the urethra-ADSC and tail vein-ADSC groups, respectively, had abnormal voiding. Histologic analysis showed that the ADSC-treated groups had significantly higher elastin content than the control group and, within the ADSC-treated groups, rats with normal voiding pattern also had significantly higher elastin content than rats with voiding dysfunction. ADSC-treated normal-voiding rats had significantly higher smooth muscle content than control or ADSC-treated rats with voiding dysfunction. CONCLUSIONS: Transplantation of ADSC via urethral or intravenous injection is effective in the treatment and/or prevention of SUI in a pre-clinical setting.
BACKGROUND AIMS: Effective treatment for stress urinary incontinence (SUI) is lacking. This study investigated whether transplantation of adipose tissue-derived stem cells (ADSC) can treat SUI in a rat model. METHODS:Rats were induced to develop SUI by postpartum vaginal balloon dilation and bilateral ovariectomy. ADSC were isolated from the peri-ovary fat, examined for stem cell properties, and labeled with thymidine analog BrdU or EdU. Ten rats received urethral injection of saline as a control. Twelve rats received urethral injection of EdU-labeled ADSC and six rats received intravenous injection of BrdU-labeled ADSC through the tail vein. Four weeks later, urinary voiding function was assessed by conscious cystometry. The rats were then killed and their urethras harvested for tracking of ADSC and quantification of elastin, collagen and smooth muscle contents. RESULTS: Cystometric analysis showed that eight out 10 rats in the control group had abnormal voiding, whereas four of 12 (33.3%) and two of six (33.3%) rats in the urethra-ADSC and tail vein-ADSC groups, respectively, had abnormal voiding. Histologic analysis showed that the ADSC-treated groups had significantly higher elastin content than the control group and, within the ADSC-treated groups, rats with normal voiding pattern also had significantly higher elastin content than rats with voiding dysfunction. ADSC-treated normal-voiding rats had significantly higher smooth muscle content than control or ADSC-treated rats with voiding dysfunction. CONCLUSIONS: Transplantation of ADSC via urethral or intravenous injection is effective in the treatment and/or prevention of SUI in a pre-clinical setting.
Authors: Brian M Strem; Kevin C Hicok; Min Zhu; Isabella Wulur; Zeni Alfonso; Ronda E Schreiber; John K Fraser; Marc H Hedrick Journal: Keio J Med Date: 2005-09
Authors: Guiting Lin; Maurice Garcia; Hongxiu Ning; Lia Banie; Ying-Lu Guo; Tom F Lue; Ching-Shwun Lin Journal: Stem Cells Dev Date: 2008-12 Impact factor: 3.272
Authors: Soren Schenk; Niladri Mal; Amanda Finan; Ming Zhang; Matt Kiedrowski; Zoran Popovic; Patrick M McCarthy; Marc S Penn Journal: Stem Cells Date: 2006-10-19 Impact factor: 6.277
Authors: Kavirach Tantiwongse; Thomas M Fandel; Guifang Wang; Benjamin N Breyer; Thomas J Walsh; Anthony J Bella; Tom F Lue Journal: BJU Int Date: 2008-07-01 Impact factor: 5.588
Authors: Guiting Lin; Alan W Shindel; Lia Banie; Donna Deng; Guifang Wang; Narihiko Hayashi; Ching-Shwun Lin; Tom F Lue Journal: Eur Urol Date: 2008-03-18 Impact factor: 20.096