Literature DB >> 19871041

OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS.

J E Smadel1, E G Pickels, T Shedlovsky, T M Rivers.   

Abstract

It has been shown experimentally that mixtures of two types of particles, namely, elementary bodies of vaccinia and collodion particles coated with protein, sediment with a single boundary in the analytical centrifuge. Such mixtures have been shown to develop one or two boundaries on electrophoresis in the Tiselius apparatus, depending on the type of coating on the surface of the collodion particles. When covered with the heat-stable soluble antigen of vaccinia, collodion particles migrate in the electrical field at the same rate as elementary bodies. On the other hand, if they are coated with a component of normal rabbit serum, they migrate at a different rate. The estimation of purity of preparations of virus by means of data obtained by ultracentrifugation and electrophoresis is discussed.

Entities:  

Year:  1940        PMID： 19871041      PMCID： PMC2135035          DOI： 10.1084/jem.72.5.523

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  6 in total

1.  ULTRACENTRIFUGATION STUDIES ON THE ELEMENTARY BODIES OF VACCINE VIRUS : II. THE INFLUENCE OF SUCROSE, GLYCEROL, AND UREA SOLUTIONS ON THE PHYSICAL NATURE OF VACCINE VIRUS.

Authors:  J E Smadel; E G Pickels; T Shedlovsky
Journal:  J Exp Med       Date:  1938-09-30       Impact factor: 14.307

2.  AN IMPROVED AIR-DRIVEN TYPE OF ULTRACENTRIFUGE FOR MOLECULAR SEDIMENTATION.

Authors:  J H Bauer; E G Pickels
Journal:  J Exp Med       Date:  1937-03-31       Impact factor: 14.307

3.  A METHOD OF STANDARDIZING BACTERIAL SUSPENSIONS.

Authors:  F L Gates
Journal:  J Exp Med       Date:  1920-01-01       Impact factor: 14.307

4.  ELECTROPHORETIC STUDIES ON ELEMENTARY BODIES OF VACCINIA.

Authors:  T Shedlovsky; J E Smadel
Journal:  J Exp Med       Date:  1940-10-31       Impact factor: 14.307

5.  ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA.

Authors:  J E Smadel; T M Rivers; E G Pickels
Journal:  J Exp Med       Date:  1939-09-30       Impact factor: 14.307

6.  ULTRACENTRIFUGATION STUDIES OF YELLOW FEVER VIRUS.

Authors:  E G Pickels; J H Bauer
Journal:  J Exp Med       Date:  1940-04-30       Impact factor: 14.307
  6 in total
  3 in total

1.  [THE DETERMINATION OF THE MIGRATION RATE OF ANIMAL VIRUSES IN THE DENSITY GRADIENT OF CARRIER-FREE ZONE ELECTROPHORESES. 1. ON THE TECHNIC AND THEORY OF CARRIER-FREE ZONE ELECTROPHORESIS IN THE GLUCOSE DENSITY GRADIENT].

Authors:  H D MATHEKA; E GEISS
Journal:  Arch Gesamte Virusforsch       Date:  1965

2.  Density gradient electrophoresis as a new tool in virology.

Authors:  R CRAMER; H SVENSSON
Journal:  Experientia       Date:  1961-02-15

3.  Two-phase formation in solutions of tobacco mosaic virus and the problem of long-range forces.

Authors:  G OSTER
Journal:  J Gen Physiol       Date:  1950-05-20       Impact factor: 4.086
  3 in total

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