Literature DB >> 19870916

ESTIMATION OF THE PURITY OF PREPARATIONS OF ELEMENTARY BODIES OF VACCINIA.

J E Smadel1, T M Rivers, E G Pickels.   

Abstract

A method of estimating the purity of preparations of elementary bodies of vaccinia is described. It depends on the comparison of the number of infective units of virus in a given material with the number of elementary bodies. The latter figure is estimated from the dry weight of the preparation by means of a calculated value for the weight of a single dehydrated elementary body. Values for the ratio of infective units of vaccine virus to elementary bodies varied between 1 : 2.4 and 1 : 9.2 in seven consecutive experiments; the average was 1:4.2. These ratios indicate a high degree of purity of the preparation. Moreover, they indicate that a relatively high percentage of the elementary bodies in the preparations was infective.

Year:  1939        PMID： 19870916      PMCID： PMC2133794          DOI： 10.1084/jem.70.4.379

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  6 in total

1.  ULTRACENTRIFUGATION STUDIES ON THE ELEMENTARY BODIES OF VACCINE VIRUS : II. THE INFLUENCE OF SUCROSE, GLYCEROL, AND UREA SOLUTIONS ON THE PHYSICAL NATURE OF VACCINE VIRUS.

Authors:  J E Smadel; E G Pickels; T Shedlovsky
Journal:  J Exp Med       Date:  1938-09-30       Impact factor: 14.307

2.  A HIGH SPEED VACUUM CENTRIFUGE SUITABLE FOR THE STUDY OF FILTERABLE VIRUSES.

Authors:  J H Bauer; E G Pickels
Journal:  J Exp Med       Date:  1936-09-30       Impact factor: 14.307

3.  IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : II. CHEMICAL ANALYSIS OF ELEMENTARY BODIES OF VACCINIA.

Authors:  T P Hughes; R F Parker; T M Rivers
Journal:  J Exp Med       Date:  1935-08-31       Impact factor: 14.307

4.  IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : I. PREPARATION OF ELEMENTARY BODIES OF VACCINIA.

Authors:  R F Parker; T M Rivers
Journal:  J Exp Med       Date:  1935-06-30       Impact factor: 14.307

5.  STATISTICAL STUDIES OF THE NATURE OF THE INFECTIOUS UNIT OF VACCINE VIRUS.

Authors:  R F Parker
Journal:  J Exp Med       Date:  1938-04-30       Impact factor: 14.307

6.  IMMUNOLOGICAL AND CHEMICAL INVESTIGATIONS OF VACCINE VIRUS : IV. STATISTICAL STUDIES OF ELEMENTARY BODIES IN RELATION TO INFECTION AND AGGLUTINATION.

Authors:  R F Parker; T M Rivers
Journal:  J Exp Med       Date:  1936-08-31       Impact factor: 14.307
  6 in total
  13 in total

1.  [Best suited method for culturing influenza, mumps and Newcastle virus in the allantois sack of a chicken embryo for the purpose of determining disinfectants].

Authors:  A GRAFE; H G HAUSSMANN
Journal:  Z Hyg Infektionskr       Date:  1957

2.  [Virus diseases in otorhinolaryngology region].

Authors:  R HAAS
Journal:  Arch Ohren Nasen Kehlkopfheilkd       Date:  1955-05-02

3.  CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : III. THE EFFECT OF PURIFIED ENZYMES ON ELEMENTARY BODIES OF VACCINIA.

Authors:  C L Hoagland; S M Ward; J E Smadel; T M Rivers
Journal:  J Exp Med       Date:  1940-11-30       Impact factor: 14.307

4.  SOME CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA.

Authors:  J E Smadel; G I Lavin; R J Dubos
Journal:  J Exp Med       Date:  1940-02-29       Impact factor: 14.307

5.  MORPHOLOGICAL STRUCTURE OF THE VIRUS OF VACCINIA.

Authors:  R H Green; T F Anderson; J E Smadel
Journal:  J Exp Med       Date:  1942-06-01       Impact factor: 14.307

6.  ELECTROPHORETIC STUDIES ON ELEMENTARY BODIES OF VACCINIA.

Authors:  T Shedlovsky; J E Smadel
Journal:  J Exp Med       Date:  1940-10-31       Impact factor: 14.307

7.  CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : II. PROPERTIES OF NUCLEIC ACID OBTAINED FROM VACCINE VIRUS.

Authors:  C L Hoagland; G I Lavin; J E Smadel; T M Rivers
Journal:  J Exp Med       Date:  1940-07-31       Impact factor: 14.307

8.  OBSERVATIONS ON MIXTURES OF ELEMENTARY BODIES OF VACCINIA AND COATED COLLODION PARTICLES BY MEANS OF ULTRACENTRIFUGATION AND ELECTROPHORESIS.

Authors:  J E Smadel; E G Pickels; T Shedlovsky; T M Rivers
Journal:  J Exp Med       Date:  1940-10-31       Impact factor: 14.307

9.  CONSTITUENTS OF ELEMENTARY BODIES OF VACCINIA : I. CERTAIN BASIC ANALYSES AND OBSERVATIONS ON LIPID COMPONENTS OF THE VIRUS.

Authors:  C L Hoagland; J E Smadel; T M Rivers
Journal:  J Exp Med       Date:  1940-05-31       Impact factor: 14.307

10.  Ratios of vaccinia virus particles to virus infectious units; studies of ratio changes during growth and adaptation in eggs, guinea pigs, and rabbits.

Authors:  J R OVERMAN; D G SHARP
Journal:  J Exp Med       Date:  1959-09-01       Impact factor: 14.307
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