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Literature DB >> 19870831

AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS.

Abstract

1. Antibody produced in the horse migrates as a new serum component between the beta and gamma components, whereas rabbit antibody is electrophoretically identical with the gamma globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the gamma globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbed and absorbed sera. Antibody solutions of high purity can be obtained by electrophoretic isolation of the gamma globulin of rabbit antisera in which the percentage of antibody to total gamma globulin is high. 3. The isoelectric points of pig, cow, horse, and rabbit antibodies have been determined. 4. In horse sera prolonged immunization is accompanied by the formation of another antibody component of lower mobility.

Entities: Species

Year: 1939 PMID： 19870831 PMCID： PMC2133726 DOI： 10.1084/jem.69.1.119

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

11 in total

1. Electrophoresis of serum globulin: Electrophoretic analysis of normal and immune sera.

Authors: A Tiselius
Journal: Biochem J Date: 1937-09 Impact factor: 3.857

2. STUDIES ON SERUM PROTEINS. I. IDENTIFICATION OF A SINGLE SERUM GLOBULIN BY IMMUNOLOGICAL MEANS. ITS DISTRIBUTION IN THE SERA OF NORMAL INDIVIDUALS AND OF PATIENTS WITH CIRRHOSIS OF THE LIVER AND WITH CHRONIC GLOMERULONEPHRITIS.

Authors: F E Kendall
Journal: J Clin Invest Date: 1937-11 Impact factor: 14.808

3. THE MOLECULAR WEIGHTS OF ANTIBODIES.

Authors: E A Kabat; K O Pedersen
Journal: Science Date: 1938-04-22 Impact factor: 47.728

4. ELECTROPHORESIS OF PURIFIED ANTIBODY PREPARATIONS.

Authors: A Tiselius
Journal: J Exp Med Date: 1937-04-30 Impact factor: 14.307

5. THE PURIFICATION OF THE ANTIBODIES IN TYPE I ANTI-PNEUMOCOCCUS SERUM, AND THE CHEMICAL NATURE OF THE TYPE-SPECIFIC PRECIPITIN REACTION.

Authors: B F Chow; W F Goebel
Journal: J Exp Med Date: 1935-07-31 Impact factor: 14.307

6. CHEMICAL STUDIES ON BACTERIAL AGGLUTINATION : I. A METHOD.

Authors: M Heidelberger; E A Kabat
Journal: J Exp Med Date: 1934-10-31 Impact factor: 14.307

7. THE MOLECULAR WEIGHT OF ANTIBODIES.

Authors: M Heidelberger; K O Pedersen
Journal: J Exp Med Date: 1937-02-28 Impact factor: 14.307

8. SPECIFIC PRECIPITATION AND MOUSE PROTECTION IN TYPE I ANTIPNEUMOCOCCUS SERA.

Authors: M Heidelberger; R H Sia; F E Kendall
Journal: J Exp Med Date: 1930-09-30 Impact factor: 14.307

9. QUANTITATIVE STUDIES ON ANTIBODY PURIFICATION : I. THE DISSOCIATION OF PRECIPITATES FORMED BY PNEUMOCOCCUS SPECIFIC POLYSACCHARIDES AND HOMOLOGOUS ANTIBODIES.

Authors: M Heidelberger; F E Kendall
Journal: J Exp Med Date: 1936-07-31 Impact factor: 14.307

10. THE SPECIFIC POLYSACCHARIDES OF TYPES I, II, AND III PNEUMOCOCCUS : A REVISION OF METHODS AND DATA.

Authors: M Heidelberger; F E Kendall; H W Scherp
Journal: J Exp Med Date: 1936-09-30 Impact factor: 14.307

41 in total

1. [Immunological study of antidiphtheria serum in the sheep. II. Study of neutralizing and non-precipitating antibodies].

Authors: E H RELYVELD; A van TRIET; M RAYNAUD
Journal: Antonie Van Leeuwenhoek Date: 1960 Impact factor: 2.271

8. [Newer method of determination and localization of antibodies; with a special reference to the combination of electrophoresis and serologic antibody-determination].

Authors: F SCHEIFFARTH; G BERG; W FRENGER
Journal: Klin Wochenschr Date: 1956-02-01

9. [Gamma globulin deficiency syndrome and body fluid defense, with special reference to agammaglobulinemia].

Authors: K W BRUCKEL; P NEUFFER; F H FRANKEN
Journal: Klin Wochenschr Date: 1956-03-15

10. [Paper electrophoresis of blood group antibodies in blood proteins fractions].

Authors: F SCHLEYER
Journal: Klin Wochenschr Date: 1954-08-15