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Literature DB >> 13319586

The localization of skin-sensitizing antibody in the sera of ragweed-sensitive individuals by electrophoresis.

Abstract

Starch electrophoresis was used to separate allergic sera into electrophoretically distinct albumin, alpha-, beta-, and gamma-globulin fractions. The identity of the various fractions was established by free and paper electrophoresis. The gamma-globulin fractions were also characterized by ultracentrifugation. The serum skin-sensitizing antibody to ragweed pollen was shown to be associated with the gamma-globulins in fourteen allergic sera by means of passive transfer technique. The mobility of the skin-sensitizing antibody corresponded to that of gamma-1 and/or gamma-2 globulins. All the other serum fractions were devoid of reaginic activity. Reaginic activity per se was not directly related to protein concentration. The discrepancies between these results, and those obtained by others and ourselves previously are discussed.

Entities: Chemical Disease Species

Keywords: ANTIGENS AND ANTIBODIES; HAY FEVER/immunology

Mesh：

Substances：

Year: 1956 PMID： 13319586 PMCID： PMC2136628 DOI： 10.1084/jem.103.5.679

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

13 in total

1. The isolation and electrophoretic characterization of the proteins in the urine of normal subjects.

Authors: E McGARRY; A H SEHON; B ROSE
Journal: J Clin Invest Date: 1955-06 Impact factor: 14.808

2. The relation of allergy reagins to electrophoretic components of serum.

Authors: D H CAMPBELL; J R CANN; T B FRIEDMAN; R BROWN
Journal: Science Date: 1954-02-26 Impact factor: 47.728

3. Immuno-serólogic studies of the immediate wheal-type allergies.

Authors: A E O MENZEL; W R KESSLER; R A COOKE; P MYERS
Journal: J Allergy Date: 1952-11

4. Partition of the skin-sensitizing antibody among alcohol-fractionated plasma proteins.

Authors: J H VAUGHAN; C B FAVOUR; I H JAFFEE
Journal: J Allergy Date: 1952-11

5. The Use of an Electrolytic Injector as a "Compensating Device" in Electrophoresis.

Authors: P Johnson; E M Shooter
Journal: Science Date: 1949-01-14 Impact factor: 47.728

6. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

7. Reagic serum fractions obtained by the electrophoresis-convection method.

Authors: D H CAMPBELL; J R CANN; T B FRIEDMAN; R A BROWN
Journal: J Allergy Date: 1950-11

8. Chemical, clinical and immunological studies on the products of human plasma fractionation XL. Quantitative separation and determination of the protein components in small amounts of normal human plasma.

Authors: W F LEVER; F R N GURD; E UROMA; R K BROWN; B A BARNES; K SCHMID; E L SCHULTZ
Journal: J Clin Invest Date: 1951-01 Impact factor: 14.808

The localization of skin-sensitizing antibody in the sera of ragweed-sensitive individuals by electrophoresis.

1. The isolation and electrophoretic characterization of the proteins in the urine of normal subjects.

2. The relation of allergy reagins to electrophoretic components of serum.

3. Immuno-serólogic studies of the immediate wheal-type allergies.

4. Partition of the skin-sensitizing antibody among alcohol-fractionated plasma proteins.

5. The Use of an Electrolytic Injector as a "Compensating Device" in Electrophoresis.

6. Protein measurement with the Folin phenol reagent.

7. Reagic serum fractions obtained by the electrophoresis-convection method.

8. Chemical, clinical and immunological studies on the products of human plasma fractionation XL. Quantitative separation and determination of the protein components in small amounts of normal human plasma.

9. Passive transfer of cold urticaria.

10. AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS.

1. Human reagins to grass pollens and moulds: their purification and physico-chemical characterization.

2. Mechanisms of hyperlipemia in experimental nephrosis.

3. Physico-chemical and immunologic studies on macroglobulins.

Review 4. Human reagins: appraisal of the properties of the antibody of immediate-type hypersensitivity.

5. The discovery of IgE 50 years later.

6. Detection of non-precipitating antibodies in sera of individuals allergic to ragweed pollen by an in vitro method.