Literature DB >> 19870767

A METHOD FOR DETERMINING THE DIFFERENTIAL SEDIMENTATION OF PROTEINS IN THE HIGH SPEED CONCENTRATION CENTRIFUGE.

T P Hughes1, E G Pickels, F L Horsfall.   

Abstract

A method is presented for determining the relative degree of sedimentation of proteins and other small particles, either in pure form or in a mixture, utilizing the concentration centrifuge of Bauer and Pickels. Chemical analysis or biological assay of material obtained at different fluid levels by means of a special sampling device is used to measure the degree of sedimentation. The results are reproducible within 10 per cent and, under the conditions of the experiments cited, are characteristic for the proteins thus far investigated.

Entities:  

Year:  1938        PMID： 19870767      PMCID： PMC2133646          DOI： 10.1084/jem.67.6.941

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  3 in total

1.  The determination of diffusion constants of proteins by a refractometric method.

Authors:  O Lamm; A Polson
Journal:  Biochem J       Date:  1936-03       Impact factor: 3.857

2.  An ultracentrifugal investigation of the serum proteins.

Authors:  A S McFarlane
Journal:  Biochem J       Date:  1935-02       Impact factor: 3.857

3.  A HIGH SPEED VACUUM CENTRIFUGE SUITABLE FOR THE STUDY OF FILTERABLE VIRUSES.

Authors:  J H Bauer; E G Pickels
Journal:  J Exp Med       Date:  1936-09-30       Impact factor: 14.307
  3 in total
  10 in total

1.  [Improved ultra centrifugal methods for the quantitative analysis of protein binding complexes].

Authors:  L KERP; S STEINHILBER
Journal:  Klin Wochenschr       Date:  1962-05-15

2.  SEDIMENTATION IN THE ANGLE CENTRIFUGE.

Authors:  E G Pickels
Journal:  J Gen Physiol       Date:  1943-01-20       Impact factor: 4.086

3.  [Procedure for the demonstration of insulin-binding antibodies using differential adsorption].

Authors:  L Kerp; S Steinhilber; H Kasemir
Journal:  Klin Wochenschr       Date:  1966-05-15

4.  Concentration, partial purification, properties, and nature of staphylocoagulase.

Authors:  M TAGER
Journal:  Yale J Biol Med       Date:  1948-05

5.  CENTRIFUGATION AND ULTRAFILTRATION STUDIES ON ALLANTOIC FLUID PREPARATIONS OF INFLUENZA VIRUS.

Authors:  W F Friedewald; E G Pickels
Journal:  J Exp Med       Date:  1944-03-01       Impact factor: 14.307

6.  ULTRACENTRIFUGATION STUDIES OF YELLOW FEVER VIRUS.

Authors:  E G Pickels; J H Bauer
Journal:  J Exp Med       Date:  1940-04-30       Impact factor: 14.307

7.  A LATENT VIRUS IN NORMAL MICE CAPABLE OF PRODUCING PNEUMONIA IN ITS NATURAL HOST.

Authors:  F L Horsfall; R G Hahn
Journal:  J Exp Med       Date:  1940-02-29       Impact factor: 14.307

8.  THE SOLUBLE MALARIAL ANTIGEN IN THE SERUM OF MONKEYS INFECTED WITH PLASMODIUM KNOWLESI.

Authors:  M D Eaton
Journal:  J Exp Med       Date:  1939-03-31       Impact factor: 14.307

9.  CENTRIFUGATION STUDIES ON PNEUMONIA VIRUS OF MICE (PVM) : THE RELATIVE SIZES OF FREE AND COMBINED VIRUS.

Authors:  E C Curnen; E G Pickels; F L Horsfall
Journal:  J Exp Med       Date:  1947-01-01       Impact factor: 14.307

10.  STUDIES ON EPIDEMIC INFLUENZA VIRUS : THE NATURE AND PROPERTIES OF THE COMPLEMENT-FIXING ANTIGEN.

Authors:  E H Lennette; F L Horsfall
Journal:  J Exp Med       Date:  1940-08-31       Impact factor: 14.307
  10 in total

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