Literature DB >> 1986377

Selection of suppressor methionyl-tRNA synthetases: mapping the tRNA anticodon binding site.

T Meinnel1, Y Mechulam, D Le Corre, M Panvert, S Blanquet, G Fayat.   

Abstract

Accurate aminoacylation of a tRNA by Escherichia coli methionyl-tRNA synthetase (MTS) is specified by the CAU anticodon. A genetic screening procedure was designed to isolate MTS mutants able to aminoacylate a methionine amber tRNA (CUA anticodon). Selected suppressor MTS enzymes all possess one or several mutations in the vicinity of Trp-461, a residue that is the major contributor to the stability of complexes formed with tRNAs having the cognate CAU anticodon. Analysis of catalytic properties of purified suppressor enzymes shows that they have acquired an additional specificity toward the amber anticodon without complete disruption of the methionine anticodon site. It is concluded that both positive and negative discrimination toward the binding of tRNA anticodon sequences is restricted to a limited region of the synthetase, residues 451-467.

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Year:  1991        PMID： 1986377      PMCID： PMC50796          DOI： 10.1073/pnas.88.1.291

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

Review 1.  Parameters for the molecular recognition of transfer RNAs.

Authors:  P Schimmel
Journal:  Biochemistry       Date:  1989-04-04       Impact factor: 3.162

2.  Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase.

Authors:  J R Sampson; A B DiRenzo; L S Behlen; O C Uhlenbeck
Journal:  Science       Date:  1989-03-10       Impact factor: 47.728

3.  Codon and amino-acid specificities of a transfer RNA are both converted by a single post-transcriptional modification.

Authors:  T Muramatsu; K Nishikawa; F Nemoto; Y Kuchino; S Nishimura; T Miyazawa; S Yokoyama
Journal:  Nature       Date:  1988-11-10       Impact factor: 49.962

4.  Anticodon switching changes the identity of methionine and valine transfer RNAs.

Authors:  L H Schulman; H Pelka
Journal:  Science       Date:  1988-11-04       Impact factor: 47.728

5.  Aminoacylation of RNA minihelices with alanine.

Authors:  C Francklyn; P Schimmel
Journal:  Nature       Date:  1989-02-02       Impact factor: 49.962

6.  Association of transfer RNA acceptor identity with a helical irregularity.

Authors:  W H McClain; Y M Chen; K Foss; J Schneider
Journal:  Science       Date:  1988-12-23       Impact factor: 47.728

7.  Fast purification of a functional elongator tRNAmet expressed from a synthetic gene in vivo.

Authors:  T Meinnel; Y Mechulam; G Fayat
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

8.  The mechanism of action of methionyl-tRNA synthetase. 3. Ion requirements and kinetic parameters of the ATP-PPi exchange and methionine-transfer reactions catalyzed by the native and trypsin-modified enzymes.

Authors:  F Lawrence; S Blanquet; M Poiret; M Robert-Gero; J P Waller
Journal:  Eur J Biochem       Date:  1973-07-02

9.  A simple structural feature is a major determinant of the identity of a transfer RNA.

Authors:  Y M Hou; P Schimmel
Journal:  Nature       Date:  1988-05-12       Impact factor: 49.962

10.  Structure of E. coli glutaminyl-tRNA synthetase complexed with tRNA(Gln) and ATP at 2.8 A resolution.

Authors:  M A Rould; J J Perona; D Söll; T A Steitz
Journal:  Science       Date:  1989-12-01       Impact factor: 47.728
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  30 in total

1.  RNA binding determinant in some class I tRNA synthetases identified by alignment-guided mutagenesis.

Authors:  A Shepard; K Shiba; P Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-10-15       Impact factor: 11.205

2.  Two distinct domains of the beta subunit of Aquifex aeolicus leucyl-tRNA synthetase are involved in tRNA binding as revealed by a three-hybrid selection.

Authors:  Yong-Gang Zheng; Hui Wei; Chen Ling; Franck Martin; Gilbert Eriani; En-Duo Wang
Journal:  Nucleic Acids Res       Date:  2004-06-18       Impact factor: 16.971

3.  Single amino acid changes in AspRS reveal alternative routes for expanding its tRNA repertoire in vivo.

Authors:  Franck Martin; Sharief Barends; Gilbert Eriani
Journal:  Nucleic Acids Res       Date:  2004-08-02       Impact factor: 16.971

4.  Functional assembly of a randomly cleaved protein.

Authors:  K Shiba; P Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-03-01       Impact factor: 11.205

5.  Enzymatic aminoacylation of sequence-specific RNA minihelices and hybrid duplexes with methionine.

Authors:  S A Martinis; P Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

6.  A study of communication pathways in methionyl- tRNA synthetase by molecular dynamics simulations and structure network analysis.

Authors:  Amit Ghosh; Saraswathi Vishveshwara
Journal:  Proc Natl Acad Sci U S A       Date:  2007-09-26       Impact factor: 11.205

7.  Intron locations and functional deletions in relation to the design and evolution of a subgroup of class I tRNA synthetases.

Authors:  P Schimmel; A Shepard; K Shiba
Journal:  Protein Sci       Date:  1992-10       Impact factor: 6.725

Review 8.  An operational RNA code for amino acids and possible relationship to genetic code.

Authors:  P Schimmel; R Giegé; D Moras; S Yokoyama
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-01       Impact factor: 11.205

9.  Diversified sequences of peptide epitope for same-RNA recognition.

Authors:  S Kim; L Ribas de Pouplana; P Schimmel
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-01       Impact factor: 11.205

10.  Switching from an induced-fit to a lock-and-key mechanism in an aminoacyl-tRNA synthetase with modified specificity.

Authors:  Emmanuelle Schmitt; I Caglar Tanrikulu; Tae Hyeon Yoo; Michel Panvert; David A Tirrell; Yves Mechulam
Journal:  J Mol Biol       Date:  2009-10-23       Impact factor: 5.469
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