Literature DB >> 19854928

Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosa.

Sasikala Anbarasan1, Janne Jänis, Marja Paloheimo, Mikko Laitaoja, Minna Vuolanto, Johanna Karimäki, Pirjo Vainiotalo, Matti Leisola, Ossi Turunen.   

Abstract

The effects of different structural features on the thermostability of Thermopolyspora flexuosa xylanase XYN10A were investigated. A C-terminal carbohydrate binding module had only a slight effect, whereas a polyhistidine tag increased the thermostability of XYN10A xylanase. In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 19854928      PMCID： PMC2798631          DOI： 10.1128/AEM.00357-09

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  32 in total

1.  A combination of weakly stabilizing mutations with a disulfide bridge in the alpha-helix region of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermal stability through synergism.

Authors:  O Turunen; K Etuaho; F Fenel; J Vehmaanperä; X Wu; J Rouvinen; M Leisola
Journal:  J Biotechnol       Date:  2001-06-01       Impact factor: 3.307

2.  Three-dimensional structures of thermophilic beta-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa. Comparison of twelve xylanases in relation to their thermal stability.

Authors:  Nina Hakulinen; Ossi Turunen; Janne Jänis; Matti Leisola; Juha Rouvinen
Journal:  Eur J Biochem       Date:  2003-04

3.  Glycoforms of beta-lactoglobulin with improved thermostability and preserved structural packing.

Authors:  Kerensa Broersen; Alphons G J Voragen; Rob J Hamer; Harmen H J De Jongh
Journal:  Biotechnol Bioeng       Date:  2004-04-05       Impact factor: 4.530

4.  Production of glycosylated thermostable Providencia rettgeri penicillin G amidase in Pichia pastoris.

Authors:  Milica Sevo; Giuliano Degrassi; Natasa Skoko; Vittorio Venturi; Goran Ljubijankić
Journal:  FEMS Yeast Res       Date:  2002-01       Impact factor: 2.796

5.  Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli.

Authors:  Viviana Job; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni
Journal:  Eur J Biochem       Date:  2002-03

6.  High-yield production of a bacterial xylanase in the filamentous fungus Trichoderma reesei requires a carrier polypeptide with an intact domain structure.

Authors:  Marja Paloheimo; Arja Mäntylä; Jarno Kallio; Pirkko Suominen
Journal:  Appl Environ Microbiol       Date:  2003-12       Impact factor: 4.792

7.  Fusion of family 2b carbohydrate-binding module increases the catalytic activity of a xylanase from Thermotoga maritima to soluble xylan.

Authors:  Farooqahmed S Kittur; Selanere L Mangala; Ahmed Abu Rus'd; Motomitsu Kitaoka; Hiroshi Tsujibo; Kiyoshi Hayashi
Journal:  FEBS Lett       Date:  2003-08-14       Impact factor: 4.124

8.  Influence of histidine on the stability and physical properties of a fully human antibody in aqueous and solid forms.

Authors:  Bei Chen; Raquel Bautista; Kwok Yu; Gerardo A Zapata; Michael G Mulkerrin; Steven M Chamow
Journal:  Pharm Res       Date:  2003-12       Impact factor: 4.200

9.  Crystal structures of decorated xylooligosaccharides bound to a family 10 xylanase from Streptomyces olivaceoviridis E-86.

Authors:  Zui Fujimoto; Satoshi Kaneko; Atsushi Kuno; Hideyuki Kobayashi; Isao Kusakabe; Hiroshi Mizuno
Journal:  J Biol Chem       Date:  2003-12-11       Impact factor: 5.157

10.  Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR.

Authors:  Sławomir Dabrowski; Birgitte Kiaer Ahring
Journal:  Protein Expr Purif       Date:  2003-09       Impact factor: 1.650
View more
  11 in total

1.  Bacterial community shift for monitoring the co-composting of oil palm empty fruit bunch and palm oil mill effluent anaerobic sludge.

Authors:  Mohd Huzairi Mohd Zainudin; Norhayati Ramli; Mohd Ali Hassan; Yoshihito Shirai; Kosuke Tashiro; Kenji Sakai; Yukihiro Tashiro
Journal:  J Ind Microbiol Biotechnol       Date:  2017-02-14       Impact factor: 3.346

2.  Significantly improving the thermostability of a hyperthermophilic GH10 family xylanase XynAF1 by semi-rational design.

Authors:  Guangqi Li; Xuan Zhou; Zhihong Li; Yunpeng Liu; Dongyang Liu; Youzhi Miao; Qun Wan; Ruifu Zhang
Journal:  Appl Microbiol Biotechnol       Date:  2021-05-20       Impact factor: 4.813

3.  PP6 regulatory subunit R1 is bidentate anchor for targeting protein phosphatase-6 to DNA-dependent protein kinase.

Authors:  Amol S Hosing; Nicholas C K Valerie; Jaroslaw Dziegielewski; David L Brautigan; James M Larner
Journal:  J Biol Chem       Date:  2012-02-01       Impact factor: 5.157

4.  Xylanase II from Trichoderma reesei QM 9414: conformational and catalytic stability to Chaotropes, Trifluoroethanol, and pH changes.

Authors:  G López; A Bañares-Hidalgo; P Estrada
Journal:  J Ind Microbiol Biotechnol       Date:  2010-09-14       Impact factor: 3.346

5.  Thermostability improvement of a streptomyces xylanase by introducing proline and glutamic acid residues.

Authors:  Kun Wang; Huiying Luo; Jian Tian; Ossi Turunen; Huoqing Huang; Pengjun Shi; Huifang Hua; Caihong Wang; Shuanghe Wang; Bin Yao
Journal:  Appl Environ Microbiol       Date:  2014-01-24       Impact factor: 4.792

6.  Thermal behaviour and tolerance to ionic liquid [emim]OAc in GH10 xylanase from Thermoascus aurantiacus SL16W.

Authors:  Niwat Chawachart; Sasikala Anbarasan; Samuel Turunen; He Li; Chartchai Khanongnuch; Michael Hummel; Herbert Sixta; Tom Granström; Saisamorn Lumyong; Ossi Turunen
Journal:  Extremophiles       Date:  2014-07-30       Impact factor: 2.395

7.  Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study.

Authors:  Gloria López; Pilar Estrada
Journal:  Enzyme Res       Date:  2014-09-07

8.  Hyperthermostable Thermotoga maritima xylanase XYN10B shows high activity at high temperatures in the presence of biomass-dissolving hydrophilic ionic liquids.

Authors:  Tianyi Yu; Sasikala Anbarasan; Yawei Wang; Kübra Telli; Aşkın Sevinç Aslan; Zhengding Su; Yin Zhou; Li Zhang; Piia Iivonen; Sami Havukainen; Tero Mentunen; Michael Hummel; Herbert Sixta; Baris Binay; Ossi Turunen; Hairong Xiong
Journal:  Extremophiles       Date:  2016-05-30       Impact factor: 2.395

9.  Analysis of the role of O-glycosylation in GH51 α-L-arabinofuranosidase from Pleurotus ostreatus.

Authors:  Antonella Amore; Annabel Serpico; Angela Amoresano; Roberto Vinciguerra; Vincenza Faraco
Journal:  Biotechnol Appl Biochem       Date:  2015-05-20       Impact factor: 2.431

10.  Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris.

Authors:  Xiaoyu Chang; Bo Xu; Yingguo Bai; Huiying Luo; Rui Ma; Pengjun Shi; Bin Yao
Journal:  PLoS One       Date:  2017-02-10       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.