Pseudomonas taiwanensis sp. nov., isolated from soil.

A novel Gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, CMS(T), isolated from soil was characterized using phenotypic and molecular taxonomic methods. 16S rRNA gene sequence analysis revealed that the organism belongs phylogenetically to the genus Pseudomonas. Pseudomonas monteilii, P. plecoglossicida and P. mosselii were the most closely related species, with 16S rRNA gene sequence similarities to the respective type strains of 99.79, 99.73 and 99.59 %. Relatively low gyrB gene sequence similarities (<90 %) and DNA-DNA reassociation values (<51 %) were obtained between the strain and its phylogenetically closest neighbours. The G+C content of strain CMS(T) was 62.7 mol%. The major cellular fatty acids were C(18 : 1) ω 7c, summed feature 3 (C(16 : 1) ω 7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(10 : 0) 3-OH. Based on the phenotypic and genetic evidence, the strain is suggested to represent a novel species, for which the name Pseudomonas taiwanensis sp. nov. is proposed. The type strain is CMS(T) (=BCRC 17751(T) =DSM 21245(T)).