Literature DB >> 19852765

A rapid method for titrating baculovirus stocks using the Sf-9 Easy Titer cell line.

Ralph Hopkins1, Dominic Esposito.   

Abstract

A new rapid method for titrating baculovirus stocks has been developed using a novel cell line Sf-9 Easy Titer (Sf-9ET). The Sf-9ET cell line has been transfected with plasmid DNA containing the enhanced green fluorescent protein (eGFP) gene under the control of the baculovirus polyhedrin promoter. When used in the titration assay, the Sf-9ET cells turn green when they are infected with baculovirus due to the activation of the polyhedrin promoter/eGFP complex by baculovirus gene products expressed during the infection. Using a 96-well plate format end-point dilution assay, baculovirus titers can be determined in three days using a fluorescence microscope.

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Year:  2009        PMID： 19852765     DOI： 10.2144/000113238

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  32 in total

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Authors:  Michael Duszenko; Lars Redecke; Celestin Nzanzu Mudogo; Benjamin Philip Sommer; Stefan Mogk; Dominik Oberthuer; Christian Betzel
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2015-07-29       Impact factor: 1.056

2.  Evaluation of the Virus Counter® for rapid baculovirus quantitation.

Authors:  Matthew M Ferris; Patricia C Stepp; Kirk A Ranno; Wafaa Mahmoud; Elizabeth Ibbitson; James Jarvis; Manon M J Cox; Kurt Christensen; Heather Votaw; Dean P Edwards; Kathy L Rowlen
Journal:  J Virol Methods       Date:  2010-10-21       Impact factor: 2.014

3.  Expression and purification of 15 kDa granulysin utilizing an insect cell secretion system.

Authors:  Michael W Finn; Carol Clayberger; Alan M Krensky
Journal:  Protein Expr Purif       Date:  2010-07-30       Impact factor: 1.650

4.  Widening the bottleneck: increasing success in protein expression and purification.

Authors:  Ralph Hopkins; Dominic Esposito; William Gillette
Journal:  J Struct Biol       Date:  2010-07-29       Impact factor: 2.867

5.  Utility of temporally distinct baculovirus promoters for constitutive and baculovirus-inducible transgene expression in transformed insect cells.

Authors:  Chi-Hung Lin; Donald L Jarvis
Journal:  J Biotechnol       Date:  2013-02-28       Impact factor: 3.307

6.  FGF8 and SHH substitute for anterior-posterior tissue interactions to induce limb regeneration.

Authors:  Eugeniu Nacu; Elena Gromberg; Catarina R Oliveira; David Drechsel; Elly M Tanaka
Journal:  Nature       Date:  2016-05-19       Impact factor: 49.962

7.  Engineering the transposition-based baculovirus expression vector system for higher efficiency protein production from insect cells.

Authors:  Jennifer L Mehalko; Dominic Esposito
Journal:  J Biotechnol       Date:  2016-09-09       Impact factor: 3.307

8.  Conserved Surface Residues on the Feline Calicivirus Capsid Are Essential for Interaction with Its Receptor Feline Junctional Adhesion Molecule A (fJAM-A).

Authors:  Zhengchun Lu; Emily D Ledgerwood; Meleana M Hinchman; Robert Dick; John S L Parker
Journal:  J Virol       Date:  2018-03-28       Impact factor: 5.103

9.  Baculovirus Transduction in Mammalian Cells Is Affected by the Production of Type I and III Interferons, Which Is Mediated Mainly by the cGAS-STING Pathway.

Authors:  Sabrina Amalfi; Guido Nicolás Molina; Romina Jimena Bevacqua; María Gabriela López; Oscar Taboga; Victoria Alfonso
Journal:  J Virol       Date:  2020-10-14       Impact factor: 5.103

10.  Manipulation of Subunit Stoichiometry in Heteromeric Membrane Proteins.

Authors:  Claudio L Morales-Perez; Colleen M Noviello; Ryan E Hibbs
Journal:  Structure       Date:  2016-03-31       Impact factor: 5.006
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