Literature DB >> 19851013

Crystallization and preliminary X-ray analysis of a D-Ala:D-Ser ligase associated with VanG-type vancomycin resistance.

Patrick Weber1, Djalal Meziane-Cherif, Ahmed Haouz, Frederick A Saul, Patrice Courvalin.   

Abstract

Acquired VanG-type resistance to vancomycin in Enterococcus faecalis BM4518 arises from inducible synthesis of peptidoglycan precursors ending in D-alanyl-D-serine, to which vancomycin exhibits low binding affinity. VanG, a D-alanine:D-serine ligase, catalyzes the ATP-dependent synthesis of the D-Ala-D-Ser dipeptide, which is incorporated into the peptidoglycan synthesis of VanG-type vancomycin-resistant strains. Here, the purification, crystallization and preliminary crystallographic analysis of VanG in complex with ADP are reported. The crystal belonged to space group P3(1)21, with unit-cell parameters a = b = 116.1, c = 177.2 A, and contained two molecules in the asymmetric unit. A complete data set has been collected to 2.35 A resolution from a single crystal under cryogenic conditions using synchrotron radiation.

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Year:  2009        PMID： 19851013      PMCID： PMC2765892          DOI： 10.1107/S1744309109033831

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  14 in total

1.  The Protein Data Bank.

Authors:  H M Berman; J Westbrook; Z Feng; G Gilliland; T N Bhat; H Weissig; I N Shindyalov; P E Bourne
Journal:  Nucleic Acids Res       Date:  2000-01-01       Impact factor: 16.971

2.  The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).

Authors:  D I Roper; T Huyton; A Vagin; G Dodson
Journal:  Proc Natl Acad Sci U S A       Date:  2000-08-01       Impact factor: 11.205

3.  Getting more from less: algorithms for rapid protein identification with multiple short peptide sequences.

Authors:  Aaron J Mackey; Timothy A J Haystead; William R Pearson
Journal:  Mol Cell Proteomics       Date:  2002-02       Impact factor: 5.911

4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  Active-site mutants of the VanC2 D-alanyl-D-serine ligase, characteristic of one vancomycin-resistant bacterial phenotype, revert towards wild-type D-alanyl-D-alanine ligases.

Authors:  V L Healy; I S Park; C T Walsh
Journal:  Chem Biol       Date:  1998-04

Review 6.  Structure, biochemistry and mechanism of action of glycopeptide antibiotics.

Authors:  P E Reynolds
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-11       Impact factor: 3.267

7.  Determinants for differential effects on D-Ala-D-lactate vs D-Ala-D-Ala formation by the VanA ligase from vancomycin-resistant enterococci.

Authors:  I A Lessard; V L Healy; I S Park; C T Walsh
Journal:  Biochemistry       Date:  1999-10-19       Impact factor: 3.162

8.  Isolation, cloning, and sequencing of the Salmonella typhimurium ddlA gene with purification and characterization of its product, D-alanine:D-alanine ligase (ADP forming).

Authors:  E Daub; L E Zawadzke; D Botstein; C T Walsh
Journal:  Biochemistry       Date:  1988-05-17       Impact factor: 3.162

9.  Evidence for in vivo incorporation of D-lactate into peptidoglycan precursors of vancomycin-resistant enterococci.

Authors:  M Arthur; C Molinas; T D Bugg; G D Wright; C T Walsh; P Courvalin
Journal:  Antimicrob Agents Chemother       Date:  1992-04       Impact factor: 5.191

10.  Phaser crystallographic software.

Authors:  Airlie J McCoy; Ralf W Grosse-Kunstleve; Paul D Adams; Martyn D Winn; Laurent C Storoni; Randy J Read
Journal:  J Appl Crystallogr       Date:  2007-07-13       Impact factor: 3.304
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  2 in total

1.  Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis.

Authors:  Djalal Meziane-Cherif; Frederick A Saul; Ahmed Haouz; Patrice Courvalin
Journal:  J Biol Chem       Date:  2012-09-11       Impact factor: 5.157

2.  D-Ala-d-Ser VanN-type transferable vancomycin resistance in Enterococcus faecium.

Authors:  François Lebreton; Florence Depardieu; Nancy Bourdon; Marguerite Fines-Guyon; Pierre Berger; Sabine Camiade; Roland Leclercq; Patrice Courvalin; Vincent Cattoir
Journal:  Antimicrob Agents Chemother       Date:  2011-08-01       Impact factor: 5.191
  2 in total

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