Literature DB >> 19850089

A self-deleting Cre-lox-ermAM cassette, Cheshire, for marker-less gene deletion in Streptococcus pneumoniae.

Liming Weng1, Indranil Biswas, Donald A Morrison.   

Abstract

Although targeted mutagenesis of Streptococcus pneumoniae is readily accomplished with the aid of natural genetic transformation and chimeric donor DNA constructs assembled in vitro, the drug resistance markers often employed for selection of recombinant products can themselves be undesirable by-products of the genetic manipulation. A new cassette carrying the erythromycin-resistance marker ermAM that can be used as a temporary marker for selection of desired recombinants is described. The cassette may subsequently be removed at will by virtue of an embedded fucose-regulated Cre recombinase gene and terminal lox66 and lox71 Cre recognition sites, with retention of 34bp from the cassette as an inert residual double-mutant lox72 site.

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Year:  2009        PMID： 19850089      PMCID： PMC2814315          DOI： 10.1016/j.mimet.2009.10.007

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  22 in total

1.  Chemical-regulated, site-specific DNA excision in transgenic plants.

Authors:  J Zuo; Q W Niu; S G Møller; N H Chua
Journal:  Nat Biotechnol       Date:  2001-02       Impact factor: 54.908

Review 2.  Excision of selectable marker genes from transgenic plants.

Authors:  Peter D Hare; Nam-Hai Chua
Journal:  Nat Biotechnol       Date:  2002-06       Impact factor: 54.908

3.  Genome engineering in Bacillus anthracis using Cre recombinase.

Authors:  Andrei P Pomerantsev; Ramakrishnan Sitaraman; Craig R Galloway; Violetta Kivovich; Stephen H Leppla
Journal:  Infect Immun       Date:  2006-01       Impact factor: 3.441

4.  Marker removal in staphylococci via Cre recombinase and different lox sites.

Authors:  Martina Leibig; Bernhard Krismer; Martina Kolb; Alexandra Friede; Friedrich Götz; Ralph Bertram
Journal:  Appl Environ Microbiol       Date:  2007-12-28       Impact factor: 4.792

5.  Competence for genetic transformation in Streptococcus pneumoniae: termination of activity of the alternative sigma factor ComX is independent of proteolysis of ComX and ComW.

Authors:  Andrew Piotrowski; Ping Luo; Donald A Morrison
Journal:  J Bacteriol       Date:  2009-03-13       Impact factor: 3.490

6.  Cre-lox-based system for multiple gene deletions and selectable-marker removal in Lactobacillus plantarum.

Authors:  Jolanda M Lambert; Roger S Bongers; Michiel Kleerebezem
Journal:  Appl Environ Microbiol       Date:  2006-12-01       Impact factor: 4.792

7.  An rpsL cassette, janus, for gene replacement through negative selection in Streptococcus pneumoniae.

Authors:  C K Sung; H Li; J P Claverys; D A Morrison
Journal:  Appl Environ Microbiol       Date:  2001-11       Impact factor: 4.792

8.  The two-component signal transduction system RR06/HK06 regulates expression of cbpA in Streptococcus pneumoniae.

Authors:  Alistair J Standish; Uwe H Stroeher; James C Paton
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-16       Impact factor: 11.205

9.  Cre/lox system and PCR-based genome engineering in Bacillus subtilis.

Authors:  Xin Yan; Hao-Jie Yu; Qing Hong; Shun-Peng Li
Journal:  Appl Environ Microbiol       Date:  2008-07-18       Impact factor: 4.792

10.  Markerless multiple-gene-deletion system for Streptococcus mutans.

Authors:  Anirban Banerjee; Indranil Biswas
Journal:  Appl Environ Microbiol       Date:  2008-02-08       Impact factor: 4.792
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  16 in total

1.  Development of a versatile procedure based on natural transformation for marker-free targeted genetic modification in Streptococcus thermophilus.

Authors:  Laetitia Fontaine; Damien Dandoy; Céline Boutry; Brigitte Delplace; Marie Henry de Frahan; Christophe Fremaux; Philippe Horvath; Patrick Boyaval; Pascal Hols
Journal:  Appl Environ Microbiol       Date:  2010-10-08       Impact factor: 4.792

2.  Transcriptional organization of pneumococcal psrP-secY2A2 and impact of GtfA and GtfB deletion on PsrP-associated virulence properties.

Authors:  Anel Lizcano; Ramya Akula Suresh Babu; Anukul T Shenoy; Alison Maren Saville; Nikhil Kumar; Adonis D'Mello; Cecilia A Hinojosa; Ryan P Gilley; Jesus Segovia; Timothy J Mitchell; Hervé Tettelin; Carlos J Orihuela
Journal:  Microbes Infect       Date:  2017-04-10       Impact factor: 2.700

3.  The pneumococcal σX activator, ComW, is a DNA-binding protein critical for natural transformation.

Authors:  Nicole L Inniss; Gerd Prehna; Donald A Morrison
Journal:  J Biol Chem       Date:  2019-06-03       Impact factor: 5.157

4.  Competence for genetic transformation in Streptococcus pneumoniae: mutations in σA bypass the comW requirement.

Authors:  Yanina Tovpeko; Donald A Morrison
Journal:  J Bacteriol       Date:  2014-08-11       Impact factor: 3.490

5.  Competence for Genetic Transformation in Streptococcus pneumoniae: Mutations in σA Bypass the ComW Requirement for Late Gene Expression.

Authors:  Yanina Tovpeko; Junqin Bai; Donald A Morrison
Journal:  J Bacteriol       Date:  2016-08-11       Impact factor: 3.490

6.  Decrease in penicillin susceptibility due to heat shock protein ClpL in Streptococcus pneumoniae.

Authors:  Thao Dang-Hien Tran; Hyog-Young Kwon; Eun-Hye Kim; Ki-Woo Kim; David E Briles; Suhkneung Pyo; Dong-Kwon Rhee
Journal:  Antimicrob Agents Chemother       Date:  2011-03-21       Impact factor: 5.191

7.  Mobilizable Rolling-Circle Replicating Plasmids from Gram-Positive Bacteria: A Low-Cost Conjugative Transfer.

Authors:  Cris Fernández-López; Alicia Bravo; Sofía Ruiz-Cruz; Virtu Solano-Collado; Danielle A Garsin; Fabián Lorenzo-Díaz; Manuel Espinosa
Journal:  Microbiol Spectr       Date:  2014-09-19

8.  Carbon catabolite repression by seryl phosphorylated HPr is essential to Streptococcus pneumoniae in carbohydrate-rich environments.

Authors:  Eleanor Fleming; David W Lazinski; Andrew Camilli
Journal:  Mol Microbiol       Date:  2015-05-15       Impact factor: 3.501

9.  ManLMN is a glucose transporter and central metabolic regulator in Streptococcus pneumoniae.

Authors:  Eleanor Fleming; Andrew Camilli
Journal:  Mol Microbiol       Date:  2016-08-18       Impact factor: 3.501

10.  Inactivated pep27 mutant as an effective mucosal vaccine against a secondary lethal pneumococcal challenge in mice.

Authors:  Sang-Yoon Choi; Thao Dang-Hien Tran; David E Briles; Dong-Kwon Rhee
Journal:  Clin Exp Vaccine Res       Date:  2013-01-15
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