Literature DB >> 20935129

Development of a versatile procedure based on natural transformation for marker-free targeted genetic modification in Streptococcus thermophilus.

Laetitia Fontaine1, Damien Dandoy, Céline Boutry, Brigitte Delplace, Marie Henry de Frahan, Christophe Fremaux, Philippe Horvath, Patrick Boyaval, Pascal Hols.   

Abstract

A versatile natural transformation protocol was established for and successfully applied to 18 of the 19 Streptococcus thermophilus strains tested. The efficiency of the protocol enables the use of in vitro-amplified mutagenesis fragments to perform deletion or insertion of large genetic fragments. Depending on the phenotype linked to the mutation, markerless mutants can be selected either in two steps, i.e., resistance marker insertion and excision using an adapted Cre-loxP system, or in one step using a powerful positive screening procedure as illustrated here for histidine prototrophy.

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Year:  2010        PMID： 20935129      PMCID： PMC2988589          DOI： 10.1128/AEM.01671-10

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  26 in total

Review 1.  New insights in the molecular biology and physiology of Streptococcus thermophilus revealed by comparative genomics.

Authors:  Pascal Hols; Frédéric Hancy; Laetitia Fontaine; Benoît Grossiord; Deborah Prozzi; Nathalie Leblond-Bourget; Bernard Decaris; Alexander Bolotin; Christine Delorme; S Dusko Ehrlich; Eric Guédon; Véronique Monnet; Pierre Renault; Michiel Kleerebezem
Journal:  FEMS Microbiol Rev       Date:  2005-08       Impact factor: 16.408

Review 2.  Molecular genetics of Streptococcus thermophilus.

Authors:  A Mercenier
Journal:  FEMS Microbiol Rev       Date:  1990-09       Impact factor: 16.408

3.  Insertion of a heterologous gene construct into a non-functional ORF of the Streptococcus thermophilus chromosome.

Authors:  John A Renye; George A Somkuti
Journal:  Biotechnol Lett       Date:  2009-01-27       Impact factor: 2.461

Review 4.  Controlling competence in Bacillus subtilis: shared use of regulators.

Authors:  Leendert W Hamoen; Gerard Venema; Oscar P Kuipers
Journal:  Microbiology (Reading)       Date:  2003-01       Impact factor: 2.777

5.  The oligopeptide transport system is essential for the development of natural competence in Streptococcus thermophilus strain LMD-9.

Authors:  Rozenn Gardan; Colette Besset; Alain Guillot; Christophe Gitton; Véronique Monnet
Journal:  J Bacteriol       Date:  2009-05-15       Impact factor: 3.490

6.  The inhibitory spectrum of thermophilin 9 from Streptococcus thermophilus LMD-9 depends on the production of multiple peptides and the activity of BlpG(St), a thiol-disulfide oxidase.

Authors:  Laetitia Fontaine; Pascal Hols
Journal:  Appl Environ Microbiol       Date:  2007-12-21       Impact factor: 4.792

7.  Unmarked gene modification in Streptococcus mutans by a cotransformation strategy with a thermosensitive plasmid.

Authors:  Indranil Biswas; Laura Drake; Sean Johnson; Damon Thielen
Journal:  Biotechniques       Date:  2007-04       Impact factor: 1.993

8.  Method for introducing specific and unmarked mutations into the chromosome of Streptococcus pneumoniae.

Authors:  Francesco Iannelli; Gianni Pozzi
Journal:  Mol Biotechnol       Date:  2004-01       Impact factor: 2.695

9.  Major role of NAD-dependent lactate dehydrogenases in aerobic lactate utilization in Lactobacillus plantarum during early stationary phase.

Authors:  Philippe Goffin; Frédérique Lorquet; Michiel Kleerebezem; Pascal Hols
Journal:  J Bacteriol       Date:  2004-10       Impact factor: 3.490

10.  Markerless multiple-gene-deletion system for Streptococcus mutans.

Authors:  Anirban Banerjee; Indranil Biswas
Journal:  Appl Environ Microbiol       Date:  2008-02-08       Impact factor: 4.792
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  21 in total

1.  Unleashing Natural Competence in Lactococcus lactis by Induction of the Competence Regulator ComX.

Authors:  Joyce Mulder; Michiel Wels; Oscar P Kuipers; Michiel Kleerebezem; Peter A Bron
Journal:  Appl Environ Microbiol       Date:  2017-09-29       Impact factor: 4.792

2.  Adaptor protein MecA is a negative regulator of the expression of late competence genes in Streptococcus thermophilus.

Authors:  Céline Boutry; Astrid Wahl; Brigitte Delplace; André Clippe; Laetitia Fontaine; Pascal Hols
Journal:  J Bacteriol       Date:  2012-01-27       Impact factor: 3.490

3.  Natural DNA Transformation Is Functional in Lactococcus lactis subsp. cremoris KW2.

Authors:  Blandine David; Amandine Radziejwoski; Frédéric Toussaint; Laetitia Fontaine; Marie Henry de Frahan; Cédric Patout; Sabine van Dillen; Patrick Boyaval; Philippe Horvath; Christophe Fremaux; Pascal Hols
Journal:  Appl Environ Microbiol       Date:  2017-08-01       Impact factor: 4.792

4.  Induction of Natural Competence in Genetically-modified Lactococcus lactis.

Authors:  Joyce Mulder; Michiel Wels; Oscar P Kuipers; Michiel Kleerebezem; Peter A Bron
Journal:  Bio Protoc       Date:  2018-07-05

5.  SOS response activation and competence development are antagonistic mechanisms in Streptococcus thermophilus.

Authors:  Céline Boutry; Brigitte Delplace; André Clippe; Laetitia Fontaine; Pascal Hols
Journal:  J Bacteriol       Date:  2012-11-30       Impact factor: 3.490

6.  Molecular dissection of pheromone selectivity in the competence signaling system ComRS of streptococci.

Authors:  Laura Ledesma-Garcia; Jordhan Thuillier; Armando Guzman-Espinola; Imke Ensinck; Inès Li de la Sierra-Gallay; Noureddine Lazar; Magali Aumont-Nicaise; Johann Mignolet; Patrice Soumillion; Sylvie Nessler; Pascal Hols
Journal:  Proc Natl Acad Sci U S A       Date:  2020-03-20       Impact factor: 11.205

7.  Analysis of the peptidoglycan hydrolase complement of Lactobacillus casei and characterization of the major γ-D-glutamyl-L-lysyl-endopeptidase.

Authors:  Krzysztof Regulski; Pascal Courtin; Mickael Meyrand; Ingmar J J Claes; Sarah Lebeer; Jos Vanderleyden; Pascal Hols; Alain Guillot; Marie-Pierre Chapot-Chartier
Journal:  PLoS One       Date:  2012-02-27       Impact factor: 3.240

8.  The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli.

Authors:  Rimantas Sapranauskas; Giedrius Gasiunas; Christophe Fremaux; Rodolphe Barrangou; Philippe Horvath; Virginijus Siksnys
Journal:  Nucleic Acids Res       Date:  2011-08-03       Impact factor: 16.971

9.  The fast milk acidifying phenotype of Streptococcus thermophilus can be acquired by natural transformation of the genomic island encoding the cell-envelope proteinase PrtS.

Authors:  Damien Dandoy; Christophe Fremaux; Marie Henry de Frahan; Philippe Horvath; Patrick Boyaval; Pascal Hols; Laetitia Fontaine
Journal:  Microb Cell Fact       Date:  2011-08-30       Impact factor: 5.328

10.  The naturally competent strain Streptococcus thermophilus LMD-9 as a new tool to anchor heterologous proteins on the cell surface.

Authors:  Xavier Lecomte; Valérie Gagnaire; Valérie Briard-Bion; Julien Jardin; Sylvie Lortal; Annie Dary; Magali Genay
Journal:  Microb Cell Fact       Date:  2014-06-05       Impact factor: 5.328
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