Literature DB >> 19837803

Polyphosphate accumulation in Escherichia coli in response to defects in DNA metabolism.

Luciana Amado1, Andrei Kuzminov.   

Abstract

Phenol-chloroform extraction of [(32)P]orthophosphate-labeled Escherichia coli cells followed by alkaline gel electrophoresis revealed, besides the expected chromosomal DNA, two non-DNA species that we have identified as lipopolysaccharides and polyphosphates by using a combination of biochemical and genetic techniques. We used this serendipitously found straightforward protocol for direct polyphosphate detection to quantify polyphosphate levels in E. coli mutants with diverse defects in the DNA metabolism. We detected increased polyphosphate accumulation in the ligA, ligA recBCD, dut ung, and thyA mutants. Polyphosphate accumulation may thus be an indicator of general DNA stress.

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Year:  2009        PMID： 19837803      PMCID： PMC2786612          DOI： 10.1128/JB.01138-09

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  34 in total

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Journal:  Annu Rev Microbiol       Date:  1998       Impact factor: 15.500

2.  Fragmentation of replicating chromosomes triggered by uracil in DNA.

Authors:  Elena A Kouzminova; Andrei Kuzminov
Journal:  J Mol Biol       Date:  2005-11-08       Impact factor: 5.469

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Journal:  Science       Date:  1997-09-05       Impact factor: 47.728

4.  The replication intermediates in Escherichia coli are not the product of DNA processing or uracil excision.

Authors:  Luciana Amado; Andrei Kuzminov
Journal:  J Biol Chem       Date:  2006-06-12       Impact factor: 5.157

Review 5.  Inorganic polyphosphate: essential for growth and survival.

Authors:  Narayana N Rao; María R Gómez-García; Arthur Kornberg
Journal:  Annu Rev Biochem       Date:  2009       Impact factor: 23.643

6.  Reduced lipopolysaccharide phosphorylation in Escherichia coli lowers the elevated ori/ter ratio in seqA mutants.

Authors:  Ella Rotman; Preston Bratcher; Andrei Kuzminov
Journal:  Mol Microbiol       Date:  2009-04-30       Impact factor: 3.501

7.  The mutT defect does not elevate chromosomal fragmentation in Escherichia coli because of the surprisingly low levels of MutM/MutY-recognized DNA modifications.

Authors:  Ella Rotman; Andrei Kuzminov
Journal:  J Bacteriol       Date:  2007-07-06       Impact factor: 3.490

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Authors:  P Pohjanpelto; E Hölttä
Journal:  EMBO J       Date:  1996-03-01       Impact factor: 11.598

9.  Involvement of waaY, waaQ, and waaP in the modification of Escherichia coli lipopolysaccharide and their role in the formation of a stable outer membrane.

Authors:  J A Yethon; D E Heinrichs; M A Monteiro; M B Perry; C Whitfield
Journal:  J Biol Chem       Date:  1998-10-09       Impact factor: 5.157

10.  Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection.

Authors:  Tomoya Baba; Takeshi Ara; Miki Hasegawa; Yuki Takai; Yoshiko Okumura; Miki Baba; Kirill A Datsenko; Masaru Tomita; Barry L Wanner; Hirotada Mori
Journal:  Mol Syst Biol       Date:  2006-02-21       Impact factor: 11.429
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  4 in total

1.  Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa.

Authors:  Lisa R Racki; Elitza I Tocheva; Michael G Dieterle; Meaghan C Sullivan; Grant J Jensen; Dianne K Newman
Journal:  Proc Natl Acad Sci U S A       Date:  2017-03-06       Impact factor: 11.205

2.  Mutations in Escherichia coli Polyphosphate Kinase That Lead to Dramatically Increased In Vivo Polyphosphate Levels.

Authors:  Amanda K Rudat; Arya Pokhrel; Todd J Green; Michael J Gray
Journal:  J Bacteriol       Date:  2018-02-23       Impact factor: 3.490

3.  phoU inactivation in Pseudomonas aeruginosa enhances accumulation of ppGpp and polyphosphate.

Authors:  Luiz Gustavo de Almeida; Julia Helena Ortiz; René P Schneider; Beny Spira
Journal:  Appl Environ Microbiol       Date:  2015-02-20       Impact factor: 4.792

4.  Low-molecular-weight DNA replication intermediates in Escherichia coli: mechanism of formation and strand specificity.

Authors:  Luciana Amado; Andrei Kuzminov
Journal:  J Mol Biol       Date:  2013-07-20       Impact factor: 5.469
  4 in total

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