| Literature DB >> 19837035 |
Holly C Dippold1, Michelle M Ng, Suzette E Farber-Katz, Sun-Kyung Lee, Monica L Kerr, Marshall C Peterman, Ronald Sim, Patricia A Wiharto, Kenneth A Galbraith, Swetha Madhavarapu, Greg J Fuchs, Timo Meerloo, Marilyn G Farquhar, Huilin Zhou, Seth J Field.
Abstract
Golgi membranes, from yeast to humans, are uniquely enriched in phosphatidylinositol-4-phosphate (PtdIns(4)P), although the role of this lipid remains poorly understood. Using a proteomic lipid-binding screen, we identify the Golgi protein GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) as a PtdIns(4)P-binding protein that depends on PtdIns(4)P for its Golgi localization. We further show that GOLPH3 binds the unconventional myosin MYO18A, thus connecting the Golgi to F-actin. We demonstrate that this linkage is necessary for normal Golgi trafficking and morphology. The evidence suggests that GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation. Consequently, this tensile force stretches the Golgi into the extended ribbon observed by fluorescence microscopy and the familiar flattened form observed by electron microscopy.Entities:
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Year: 2009 PMID： 19837035 PMCID： PMC2779841 DOI： 10.1016/j.cell.2009.07.052
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582