Literature DB >> 1982991

Modifications to improve the effectiveness of restriction fragment length polymorphism typing.

B Budowle1, F S Baechtel.   

Abstract

A streamlined and effective method for RFLP analysis of DNA has been developed. Southern transfers are accomplished by alkali blotting DNA onto positively charged nylon membranes. The prehybridization step has been eliminated. The hybridization solution is composed of three cost-effective reagents: 7% SDS, 10% PEG, and phosphate buffer. By using probes that hybridize to variable number of tandem repeat loci, and RFLP analysis of one to two micrograms of genomic DNA can be achieved within five working days under normal working hours. With longer autoradiographic exposures, as little as 20-100 ng of human genomic DNA is sufficient for analysis.

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Year:  1990        PMID： 1982991

Source DB:  PubMed          Journal:  Appl Theor Electrophor        ISSN： 0954-6642


  21 in total

Review 1.  Human identity testing with PCR-based systems.

Authors:  C Hohoff; B Brinkmann
Journal:  Mol Biotechnol       Date:  1999-12-01       Impact factor: 2.695

2.  Independence of VNTR alleles defined as fixed bins.

Authors:  B S Weir
Journal:  Genetics       Date:  1992-04       Impact factor: 4.562

3.  Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE.

Authors:  B Budowle; R Chakraborty; A M Giusti; A J Eisenberg; R C Allen
Journal:  Am J Hum Genet       Date:  1991-01       Impact factor: 11.025

4.  Fixed-bin analysis for statistical evaluation of continuous distributions of allelic data from VNTR loci, for use in forensic comparisons.

Authors:  B Budowle; A M Giusti; J S Waye; F S Baechtel; R M Fourney; D E Adams; L A Presley; H A Deadman; K L Monson
Journal:  Am J Hum Genet       Date:  1991-05       Impact factor: 11.025

5.  Phosphatidylinositol 3-kinase (PI3K) signaling via glycogen synthase kinase-3 (Gsk-3) regulates DNA methylation of imprinted loci.

Authors:  Anthony P Popkie; Leigh C Zeidner; Ashley M Albrecht; Anthony D'Ippolito; Sigrid Eckardt; David E Newsom; Joanna Groden; Bradley W Doble; Bruce Aronow; K John McLaughlin; Peter White; Christopher J Phiel
Journal:  J Biol Chem       Date:  2010-11-03       Impact factor: 5.157

6.  PCR analysis of the short tandem repeat (STR) system HUMVWA31. Allele and genotype frequencies in an Italian population sample.

Authors:  L Buscemi; N Cucurachi; R Mencarelli; A Tagliabracci; P Wiegand; S D Ferrara
Journal:  Int J Legal Med       Date:  1995       Impact factor: 2.686

7.  Suitability of PCR methods for forensic investigation. Analysis of the 3'apoB VNTR system in an Italian population sample.

Authors:  R Giorgetti; A Tagliabracci; A Agostini; M Cingolani; S D Ferrara
Journal:  Int J Legal Med       Date:  1991       Impact factor: 2.686

8.  Co-receptor choice by V alpha14i NKT cells is driven by Th-POK expression rather than avoidance of CD8-mediated negative selection.

Authors:  Isaac Engel; Kirsten Hammond; Barbara A Sullivan; Xi He; Ichiro Taniuchi; Dietmar Kappes; Mitchell Kronenberg
Journal:  J Exp Med       Date:  2010-04-19       Impact factor: 14.307

9.  Analysis in a large hyperkalemic periodic paralysis pedigree supports tight linkage to a sodium channel locus.

Authors:  L J Ptacek; F Tyler; J S Trimmer; W S Agnew; M Leppert
Journal:  Am J Hum Genet       Date:  1991-08       Impact factor: 11.025

10.  Cloning, sequencing, and functional analysis of a Marek's disease virus origin of DNA replication.

Authors:  H S Camp; P M Coussens; R F Silva
Journal:  J Virol       Date:  1991-11       Impact factor: 5.103
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