AIMS: The peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PPARGC1A) is a transcriptional factor that regulates the genes involved in energy metabolism. Single nucleotide polymorphisms (SNPs) in the PPARGC1A gene have been conflictingly reported to be associated with type 2 diabetes (T2D) in several populations. The present study aimed to evaluate the association of PPARGC1A genetic polymorphisms with T2D in Han Chinese population. METHODS: SNPs in the PPARGC1A gene were validated and six valid SNPs, including rs13131226, rs7656250, rs2970856, rs8192678, rs3736265 and rs3774923 were genotyped in 1090 subjects (595 patients and 495 controls) with a protocol of PCR-restriction fragment length polymorphism (PCR-RFLP). RESULTS: Single marker association analysis was conducted and there was no significant association between the PPARGC1A polymorphisms and T2D in Han Chinese population. Haplotype analysis with multiple loci, however, indicated that a common haplotype C-T-T-G-C-G was significantly associated with the increased risk of T2D (P=0.001, OR=1.80, 95%CI 1.24-2.63). There was no significant association between genotypes or haplotypes of PPARGC1A and T2D related phenotypes. CONCLUSION: The present study provides evidence that a common haplotype of PPARGC1A genetic polymorphisms are moderately associated with T2D in Han Chinese population.
AIMS: The peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PPARGC1A) is a transcriptional factor that regulates the genes involved in energy metabolism. Single nucleotide polymorphisms (SNPs) in the PPARGC1A gene have been conflictingly reported to be associated with type 2 diabetes (T2D) in several populations. The present study aimed to evaluate the association of PPARGC1A genetic polymorphisms with T2D in Han Chinese population. METHODS: SNPs in the PPARGC1A gene were validated and six valid SNPs, including rs13131226, rs7656250, rs2970856, rs8192678, rs3736265 and rs3774923 were genotyped in 1090 subjects (595 patients and 495 controls) with a protocol of PCR-restriction fragment length polymorphism (PCR-RFLP). RESULTS: Single marker association analysis was conducted and there was no significant association between the PPARGC1A polymorphisms and T2D in Han Chinese population. Haplotype analysis with multiple loci, however, indicated that a common haplotype C-T-T-G-C-G was significantly associated with the increased risk of T2D (P=0.001, OR=1.80, 95%CI 1.24-2.63). There was no significant association between genotypes or haplotypes of PPARGC1A and T2D related phenotypes. CONCLUSION: The present study provides evidence that a common haplotype of PPARGC1A genetic polymorphisms are moderately associated with T2D in Han Chinese population.
Authors: Amanpreet K Cheema; Tan Li; Juan P Liuzzi; Gustavo G Zarini; Mehmet T Dorak; Fatma G Huffman Journal: J Diabetes Res Date: 2015-04-21 Impact factor: 4.011
Authors: Adrián Montes-de-Oca-García; Juan Corral-Pérez; Daniel Velázquez-Díaz; Alejandro Perez-Bey; María Rebollo-Ramos; Alberto Marín-Galindo; Félix Gómez-Gallego; Maria Calderon-Dominguez; Cristina Casals; Jesús G Ponce-González Journal: Front Physiol Date: 2022-07-22 Impact factor: 4.755