| Literature DB >> 19822090 |
K Wang1, Y Chen, S Liu, S Qiu, S Gao, X Huang, J Zhang, X Peng, W Qiani, J Y Zhang.
Abstract
In order to understand the immunogenicity of a tumor-associated antigen (TAA), Ras family small GTP binding protein (Ra1A) in hepatocellular carcinoma (HCC), autoantibody responses to RalA were evaluated by enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay in sera from patients with HCC and sera from normal individuals. Immunohistochemistry (IHC) study with tissue array slides was also performed to analyze protein expression profiles of RalA in HCC and control tissues. This study demonstrated that RalA had a relative higher frequency of autoantibody response in HCC (20.1%) compared to liver cirrhosis (3.3%), chronic hepatitis (0%), and normal individuals sera (0%). RalA also showed a stepwise increased expression from normal liver tissues (26.7%), liver cirrhosis tissues (45.0%) to HCC tissues (63.3%). Sensitivity and specificity of anti-RalA antibody in detection of HCC was 20.1% and 99.3%, respectively. The data suggested that RalA might contribute to liver malignant transformation, and could be used as a potential tumor marker in HCC detection.Entities:
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Year: 2009 PMID： 19822090 PMCID： PMC2839122 DOI： 10.1177/039463200902200319
Source DB: PubMed Journal: Int J Immunopathol Pharmacol ISSN： 0394-6320 Impact factor: 3.219