OBJECTIVE: The objective of this study was to investigate the regulatory effect of fish oil rich in omega-3 polyunsaturated fatty acids (PUFAs) on critical enzyme activity and mRNA expression involved in homocysteine (Hcy) metabolism. METHODS: Thirty-six male Sprague-Dawley rats aged 3 wk, weighing 120 +/- 10 g, were randomly divided into three groups: the olive oil (OO) group, the tuna oil (TO) group, and the salmon oil (SO) group. The oil was orally administered every day using a stomach tube. Eight weeks later, plasma Hcy, phospholipids, omega-3 PUFAs, enzyme activity, and mRNA expression in tissues were determined. RESULTS: Compared with the control group, phospholipids, total omega-3 PUFAs, and omega-3/omega-6 PUFAs in the liver and lung were significantly elevated in the TO and SO groups; 22:6omega-3 in the liver and lung was significantly increased in the TO group; and 20:5omega-3 in the two tissues was significantly elevated in the SO group. The level of plasma Hcy was significantly decreased with TO; methionine adenosyl transferase (MAT) activity was significantly increased and MAT mRNA expression was significantly upregulated with TO; cystathionine-gamma-lyase mRNA expression in TO was significantly upregulated; however, cystathionine beta-synthase and S-adenosylhomocysteine hydrolases were not significantly changed when compared with control. CONCLUSION: TO rich in 22:6omega-3 decreases the concentration of Hcy despite increasing MAT activity and upregulating MAT mRNA expression through compensatory cystathionine-gamma-lyase mRNA expression, both of which are involved in Hcy metabolism.
OBJECTIVE: The objective of this study was to investigate the regulatory effect of fish oil rich in omega-3 polyunsaturated fatty acids (PUFAs) on critical enzyme activity and mRNA expression involved in homocysteine (Hcy) metabolism. METHODS: Thirty-six male Sprague-Dawley rats aged 3 wk, weighing 120 +/- 10 g, were randomly divided into three groups: the oliveoil (OO) group, the tunaoil (TO) group, and the salmon oil (SO) group. The oil was orally administered every day using a stomach tube. Eight weeks later, plasma Hcy, phospholipids, omega-3 PUFAs, enzyme activity, and mRNA expression in tissues were determined. RESULTS: Compared with the control group, phospholipids, total omega-3 PUFAs, and omega-3/omega-6 PUFAs in the liver and lung were significantly elevated in the TO and SO groups; 22:6omega-3 in the liver and lung was significantly increased in the TO group; and 20:5omega-3 in the two tissues was significantly elevated in the SO group. The level of plasma Hcy was significantly decreased with TO; methionine adenosyl transferase (MAT) activity was significantly increased and MAT mRNA expression was significantly upregulated with TO; cystathionine-gamma-lyase mRNA expression in TO was significantly upregulated; however, cystathionine beta-synthase and S-adenosylhomocysteine hydrolases were not significantly changed when compared with control. CONCLUSION: TO rich in 22:6omega-3 decreases the concentration of Hcy despite increasing MAT activity and upregulating MAT mRNA expression through compensatory cystathionine-gamma-lyase mRNA expression, both of which are involved in Hcy metabolism.
Authors: Tao Huang; Katherine L Tucker; Yu-Chi Lee; Jimmy W Crott; Laurence D Parnell; Jian Shen; Caren E Smith; Jose M Ordovas; Duo Li; Chao-Qiang Lai Journal: J Nutr Date: 2011-01-26 Impact factor: 4.798
Authors: T Huang; K Tucker; Y Lee; J Crott; L Parnell; J Shen; C Smith; J Ordovas; D Li; C Lai Journal: Nutr Metab Cardiovasc Dis Date: 2010-12-24 Impact factor: 4.222
Authors: Tao Huang; Katherine L Tucker; Yu-Chi Lee; Jimmy W Crott; Laurence D Parnell; Jian Shen; Caren E Smith; Jose M Ordovas; Duo Li; Chao-Qiang Lai Journal: Public Health Nutr Date: 2011-02-22 Impact factor: 4.022