| Literature DB >> 19799183 |
Matthew J Neale1, Scott Keeney.
Abstract
During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5' ends on both sides of the break during this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.Entities:
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Year: 2009 PMID： 19799183 PMCID： PMC3162315 DOI： 10.1007/978-1-59745-527-5_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745