OBJECTIVE: To assess fluoxetine effects on mitochondrial structure of the right ventricle in rats exposed to cold stress. METHODS: The experimental study procedures were performed in 250-300g male EPM-Wistar rats. Rats (n=40) were divided into four groups: 1) Control group (CON); 2) Fluoxetine (FLU); 3) Induced hypothermia (IH) and; 4) Induced hypothermia treated with fluoxetine (IHF). Animals of FLU group were treated by the administration of gavages containing 0.75 mg/kg/day fluoxetine during 40 days. The induced hypothermia was obtained by maintaining the groups 3 and 4 in a freezer at -8 degrees C for 4 hours. The animals were sacrificed and fragments of the right ventricle (RV) were removed and processed prior to performing electron microscopic analysis. RESULTS: The ultrastructural changes in cardiomyocytes were quantified through the number of mitochondrial cristae pattern (cristolysis). The CON (3.85%), FLU (4.47%) and IHF (8.4%) groups showed a normal cellular structure aspect with preserved cardiomyocytes cytoarchitecture and continuous sarcoplasmic membrane integrity. On the other hand, the IH (34.4%) group showed mitochondrial edema and lysis in cristae. CONCLUSION: The ultrastructural analysis revealed that fluoxetine strongly prevents mitochondrial cristolysis in rat heart, suggesting a protector effect under cold stress condition.
OBJECTIVE: To assess fluoxetine effects on mitochondrial structure of the right ventricle in rats exposed to cold stress. METHODS: The experimental study procedures were performed in 250-300g male EPM-Wistar rats. Rats (n=40) were divided into four groups: 1) Control group (CON); 2) Fluoxetine (FLU); 3) Induced hypothermia (IH) and; 4) Induced hypothermia treated with fluoxetine (IHF). Animals of FLU group were treated by the administration of gavages containing 0.75 mg/kg/day fluoxetine during 40 days. The induced hypothermia was obtained by maintaining the groups 3 and 4 in a freezer at -8 degrees C for 4 hours. The animals were sacrificed and fragments of the right ventricle (RV) were removed and processed prior to performing electron microscopic analysis. RESULTS: The ultrastructural changes in cardiomyocytes were quantified through the number of mitochondrial cristae pattern (cristolysis). The CON (3.85%), FLU (4.47%) and IHF (8.4%) groups showed a normal cellular structure aspect with preserved cardiomyocytes cytoarchitecture and continuous sarcoplasmic membrane integrity. On the other hand, the IH (34.4%) group showed mitochondrial edema and lysis in cristae. CONCLUSION: The ultrastructural analysis revealed that fluoxetine strongly prevents mitochondrial cristolysis in rat heart, suggesting a protector effect under cold stress condition.
Authors: José R Cisternas; Vitor E Valenti; Thales B Alves; Celso Ferreira; Márcio Petenusso; João R Breda; Adilson C Pires; Nadir Tassi; Luiz Carlos de Abreu Journal: Int Arch Med Date: 2010-01-27
Authors: Marcelo Ferreira; Paulo Roberto Santos-Silva; Luiz Carlos de Abreu; Vitor E Valenti; Vanessa Crispim; Caio Imaizumi; Celso Ferreira Filho; Neif Murad; Adriano Meneghini; Andrés R Pérez Riera; Tatiana Dias de Carvalho; Luiz Carlos Marques Vanderlei; Erica E Valenti; José R Cisternas; Oseas F Moura Filho; Celso Ferreira Journal: Sports Med Arthrosc Rehabil Ther Technol Date: 2010-08-03
Authors: Marcelo Ferreira; Celso Ferreira; Luiz Carlos de Abreu; Vitor E Valenti; Neif Murad; Adriano Meneghini; Celso F Filho; Japy Angelini de Oliveira Filho Journal: Int Arch Med Date: 2009-10-23
Authors: Guerino Barbalaco Neto; Claudia Rossetti; Natalia A Souza; Fernando LA Fonseca; Ligia Ajaime Azzalis; Virginia Berlanga Campos Junqueira; Vitor E Valenti; Luiz Carlos de Abreu Journal: Eur J Med Res Date: 2012-04-25 Impact factor: 2.175
Authors: Marcelo Ferreira; Vitor E Valenti; Jose R Cisternas; Celso Ferreira; Adriano Meneghini; Celso Ferreira Filho; João R Breda; João A Correa; Carlos Bandeira de Mello Monteiro; Hugo Macedo Junior; Neif Murad; Luiz Carlos de Abreu Journal: Int Arch Med Date: 2011-01-22