Literature DB >> 19767421

Real-time fluorescence detection of exoribonucleases.

Flore Sinturel1, Olivier Pellegrini, Song Xiang, Liang Tong, Ciarán Condon, Lionel Bénard.   

Abstract

The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay of known and unknown exoribonucleases.

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Year:  2009        PMID： 19767421      PMCID： PMC2764478          DOI： 10.1261/rna.1670909

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  10 in total

1.  Studies on three E. coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases.

Authors:  Thierry Bizebard; Ilaria Ferlenghi; Isabelle Iost; Marc Dreyfus
Journal:  Biochemistry       Date:  2004-06-22       Impact factor: 3.162

2.  Ribosomal protein L3 bound to 23S precursor rRNA stimulates its maturation by Mini-III ribonuclease.

Authors:  Yulia Redko; Ciarán Condon
Journal:  Mol Microbiol       Date:  2009-01-16       Impact factor: 3.501

3.  In vitro assays of 5' to 3'-exoribonuclease activity.

Authors:  Olivier Pellegrini; Nathalie Mathy; Ciarán Condon; Lionel Bénard
Journal:  Methods Enzymol       Date:  2008       Impact factor: 1.600

4.  Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p.

Authors:  Y Xue; X Bai; I Lee; G Kallstrom; J Ho; J Brown; A Stevens; A W Johnson
Journal:  Mol Cell Biol       Date:  2000-06       Impact factor: 4.272

5.  Lithium toxicity in yeast is due to the inhibition of RNA processing enzymes.

Authors:  B Dichtl; A Stevens; D Tollervey
Journal:  EMBO J       Date:  1997-12-01       Impact factor: 11.598

6.  Sodium-induced GCN4 expression controls the accumulation of the 5' to 3' RNA degradation inhibitor, 3'-phosphoadenosine 5'-phosphate.

Authors:  Anne-Laure Todeschini; Ciarán Condon; Lionel Bénard
Journal:  J Biol Chem       Date:  2005-12-13       Impact factor: 5.157

7.  Catalytic activation of multimeric RNase E and RNase G by 5'-monophosphorylated RNA.

Authors:  Xunqing Jiang; Joel G Belasco
Journal:  Proc Natl Acad Sci U S A       Date:  2004-06-14       Impact factor: 11.205

8.  5'-to-3' exoribonuclease activity in bacteria: role of RNase J1 in rRNA maturation and 5' stability of mRNA.

Authors:  Nathalie Mathy; Lionel Bénard; Olivier Pellegrini; Roula Daou; Tingyi Wen; Ciarán Condon
Journal:  Cell       Date:  2007-05-18       Impact factor: 41.582

9.  Assay of Bacillus subtilis ribonucleases in vitro.

Authors:  Ciarán Condon; Olivier Pellegrini; Nathalie Mathy; Lionel Bénard; Yulia Redko; Irina A Oussenko; Gintaras Deikus; David H Bechhofer
Journal:  Methods Enzymol       Date:  2008       Impact factor: 1.600

10.  Structure and function of the 5'-->3' exoribonuclease Rat1 and its activating partner Rai1.

Authors:  Song Xiang; Amalene Cooper-Morgan; Xinfu Jiao; Megerditch Kiledjian; James L Manley; Liang Tong
Journal:  Nature       Date:  2009-02-04       Impact factor: 49.962
  10 in total
  11 in total

1.  A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.

Authors:  Xinfu Jiao; Jeong Ho Chang; Turgay Kilic; Liang Tong; Megerditch Kiledjian
Journal:  Mol Cell       Date:  2013-03-21       Impact factor: 17.970

2.  Molecular mechanism for the inhibition of DXO by adenosine 3',5'-bisphosphate.

Authors:  Ji-Sook Yun; Je-Hyun Yoon; Young Jun Choi; Young Jin Son; Sunghwan Kim; Liang Tong; Jeong Ho Chang
Journal:  Biochem Biophys Res Commun       Date:  2018-09-01       Impact factor: 3.575

3.  Activation of 5'-3' exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast.

Authors:  Flore Sinturel; Dominique Bréchemier-Baey; Megerditch Kiledjian; Ciarán Condon; Lionel Bénard
Journal:  Proc Natl Acad Sci U S A       Date:  2012-05-08       Impact factor: 11.205

4.  P-TEFb regulation of transcription termination factor Xrn2 revealed by a chemical genetic screen for Cdk9 substrates.

Authors:  Miriam Sansó; Rebecca S Levin; Jesse J Lipp; Vivien Ya-Fan Wang; Ann Katrin Greifenberg; Elizabeth M Quezada; Akbar Ali; Animesh Ghosh; Stéphane Larochelle; Tariq M Rana; Matthias Geyer; Liang Tong; Kevan M Shokat; Robert P Fisher
Journal:  Genes Dev       Date:  2016-01-01       Impact factor: 11.361

5.  Highly Multiplexed RNA Aptamer Selection using a Microplate-based Microcolumn Device.

Authors:  Sarah J Reinholt; Abdullah Ozer; John T Lis; Harold G Craighead
Journal:  Sci Rep       Date:  2016-07-19       Impact factor: 4.379

6.  Functional studies of E. faecalis RNase J2 and its role in virulence and fitness.

Authors:  Peng Gao; Kenneth L Pinkston; Agathe Bourgogne; Barbara E Murray; Ambro van Hoof; Barrett R Harvey
Journal:  PLoS One       Date:  2017-04-06       Impact factor: 3.240

7.  A novel 5'-hydroxyl dinucleotide hydrolase activity for the DXO/Rai1 family of enzymes.

Authors:  Selom K Doamekpor; Agnieszka Gozdek; Aleksandra Kwasnik; Joanna Kufel; Liang Tong
Journal:  Nucleic Acids Res       Date:  2020-01-10       Impact factor: 16.971

8.  Ribonuclease J-Mediated mRNA Turnover Modulates Cell Shape, Metabolism and Virulence in Corynebacterium diphtheriae.

Authors:  Truc Thanh Luong; Minh Tan Nguyen; Yi-Wei Chen; Chungyu Chang; Ju Huck Lee; Manuel Wittchen; HyLam Ton-That; Melissa Cruz; Danielle A Garsin; Asis Das; Andreas Tauch; Hung Ton-That
Journal:  Microorganisms       Date:  2021-02-14

9.  Dxo1 is a new type of eukaryotic enzyme with both decapping and 5'-3' exoribonuclease activity.

Authors:  Jeong Ho Chang; Xinfu Jiao; Kunitoshi Chiba; ChanSeok Oh; Charles E Martin; Megerditch Kiledjian; Liang Tong
Journal:  Nat Struct Mol Biol       Date:  2012-09-09       Impact factor: 15.369

10.  Structural basis and function of XRN2 binding by XTB domains.

Authors:  Hannes Richter; Iskra Katic; Heinz Gut; Helge Großhans
Journal:  Nat Struct Mol Biol       Date:  2016-01-18       Impact factor: 15.369
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