Literature DB >> 19763943

Single-molecule magnetic tweezers studies of type IB topoisomerases.

Jan Lipfert1, Daniel A Koster, Igor D Vilfan, Susanne Hage, Nynke H Dekker.   

Abstract

The past few years have seen the application of single-molecule force spectroscopy techniques to the study of topoisomerases. Magnetic tweezers are particularly suited to the study of topoisomerases due to their unique ability to exert precise and straightforward control of the supercoiled state of DNA. Here, we illustrate in a stepwise fashion how the dynamic properties of type IB topoisomerases can be monitored using this technique.

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Year:  2009        PMID： 19763943     DOI： 10.1007/978-1-60761-340-4_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Quantitative guidelines for force calibration through spectral analysis of magnetic tweezers data.

Authors:  Aartjan J W te Velthuis; Jacob W J Kerssemakers; Jan Lipfert; Nynke H Dekker
Journal:  Biophys J       Date:  2010-08-09       Impact factor: 4.033

2.  Magnetic torque tweezers: measuring torsional stiffness in DNA and RecA-DNA filaments.

Authors:  Jan Lipfert; Jacob W J Kerssemakers; Tessa Jager; Nynke H Dekker
Journal:  Nat Methods       Date:  2010-10-17       Impact factor: 28.547

3.  Invincible DNA tethers: covalent DNA anchoring for enhanced temporal and force stability in magnetic tweezers experiments.

Authors:  Richard Janissen; Bojk A Berghuis; David Dulin; Max Wink; Theo van Laar; Nynke H Dekker
Journal:  Nucleic Acids Res       Date:  2014-08-19       Impact factor: 16.971

4.  The temperature dependence of the helical twist of DNA.

Authors:  Franziska Kriegel; Christian Matek; Tomáš Dršata; Klara Kulenkampff; Sophie Tschirpke; Martin Zacharias; Filip Lankaš; Jan Lipfert
Journal:  Nucleic Acids Res       Date:  2018-09-06       Impact factor: 16.971

5.  Probing the salt dependence of the torsional stiffness of DNA by multiplexed magnetic torque tweezers.

Authors:  Franziska Kriegel; Niklas Ermann; Ruaridh Forbes; David Dulin; Nynke H Dekker; Jan Lipfert
Journal:  Nucleic Acids Res       Date:  2017-06-02       Impact factor: 16.971

6.  Quantitative parameters of bacterial RNA polymerase open-complex formation, stabilization and disruption on a consensus promoter.

Authors:  Subhas C Bera; Pim P B America; Santeri Maatsola; Mona Seifert; Eugeniu Ostrofet; Jelmer Cnossen; Monika Spermann; Flávia S Papini; Martin Depken; Anssi M Malinen; David Dulin
Journal:  Nucleic Acids Res       Date:  2022-07-22       Impact factor: 19.160

7.  Torsional sensing of small-molecule binding using magnetic tweezers.

Authors:  Jan Lipfert; Sven Klijnhout; Nynke H Dekker
Journal:  Nucleic Acids Res       Date:  2010-07-12       Impact factor: 16.971

8.  Structural and torsional properties of the RAD51-dsDNA nucleoprotein filament.

Authors:  Mina Lee; Jan Lipfert; Humberto Sanchez; Claire Wyman; Nynke H Dekker
Journal:  Nucleic Acids Res       Date:  2013-05-22       Impact factor: 16.971

9.  Torsionally constrained DNA for single-molecule assays: an efficient, ligation-free method.

Authors:  D Hern Paik; Violet A Roskens; Thomas T Perkins
Journal:  Nucleic Acids Res       Date:  2013-08-09       Impact factor: 16.971

10.  Magnetic tweezers for the measurement of twist and torque.

Authors:  Jan Lipfert; Mina Lee; Orkide Ordu; Jacob W J Kerssemakers; Nynke H Dekker
Journal:  J Vis Exp       Date:  2014-05-19       Impact factor: 1.355
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