Literature DB >> 19741082

Comparison of a commercial multiplex real-time PCR to the cell cytotoxicity neutralization assay for diagnosis of clostridium difficile infections.

Haihui Huang1, Andrej Weintraub, Hong Fang, Carl Erik Nord.   

Abstract

A commercial multiplex real-time PCR assay (Cepheid Xpert C. difficile assay) for the diagnosis of Clostridium difficile infection was evaluated. The sensitivity and specificity of the Cepheid assay were 97.1% and 93.0% for fresh stools, using the cell cytotoxicity neutralization assay as the reference. Using PCR ribotyping as the reference for ribotype 027 strains, the corresponding figures were 100% and 98.1%, respectively.

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Year:  2009        PMID： 19741082      PMCID： PMC2772590          DOI： 10.1128/JCM.01280-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  22 in total

1.  Rapid diagnosis of toxinogenic Clostridium difficile in faecal samples with internally controlled real-time PCR.

Authors:  R J van den Berg; E J Kuijper; L E S Bruijnesteijn van Coppenraet; E C J Claas
Journal:  Clin Microbiol Infect       Date:  2006-02       Impact factor: 8.067

2.  Variant toxin B and a functional toxin A produced by Clostridium difficile C34.

Authors:  M Mehlig; M Moos; V Braun; B Kalt; D E Mahony; C von Eichel-Streiber
Journal:  FEMS Microbiol Lett       Date:  2001-05-01       Impact factor: 2.742

3.  Diagnosis of Clostridium difficile infection by toxin detection kits.

Authors:  Ian J Eltringham
Journal:  Lancet Infect Dis       Date:  2009-03       Impact factor: 25.071

4.  Evaluation of real-time PCR and conventional diagnostic methods for the detection of Clostridium difficile-associated diarrhoea in a prospective multicentre study.

Authors:  Renate J van den Berg; Norbert Vaessen; Hubert P Endtz; Tanja Schülin; Eric R van der Vorm; Ed J Kuijper
Journal:  J Med Microbiol       Date:  2007-01       Impact factor: 2.472

5.  PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes.

Authors:  S L Stubbs; J S Brazier; G L O'Neill; B I Duerden
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

6.  Coexistence of multiple PCR-ribotype strains of Clostridium difficile in faecal samples limits epidemiological studies.

Authors:  Renate J van den Berg; Hadi Aa Ameen; Takahiro Furusawa; Eric Cj Claas; Eric R van der Vorm; Ed J Kuijper
Journal:  J Med Microbiol       Date:  2005-02       Impact factor: 2.472

Review 7.  Clostridium difficile infection.

Authors:  C P Kelly; J T LaMont
Journal:  Annu Rev Med       Date:  1998       Impact factor: 13.739

Review 8.  The role of the clinical microbiology laboratory in the management of Clostridium difficile-associated diarrhea.

Authors:  L R Peterson; P J Kelly
Journal:  Infect Dis Clin North Am       Date:  1993-06       Impact factor: 5.982

Review 9.  Clinical recognition and diagnosis of Clostridium difficile infection.

Authors:  John G Bartlett; Dale N Gerding
Journal:  Clin Infect Dis       Date:  2008-01-15       Impact factor: 9.079

10.  Laboratory detection of Clostridium difficile. A comparison of media and incubation systems.

Authors:  L S Mundy; C J Shanholtzer; K E Willard; D N Gerding; L R Peterson
Journal:  Am J Clin Pathol       Date:  1995-01       Impact factor: 2.493
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  31 in total

1.  Comparison of GenomEra C. difficile and Xpert C. difficile as confirmatory tests in a multistep algorithm for diagnosis of Clostridium difficile infection.

Authors:  Luis Alcalá; Elena Reigadas; Mercedes Marín; Antonia Fernández-Chico; Pilar Catalán; Emilio Bouza
Journal:  J Clin Microbiol       Date:  2014-11-12       Impact factor: 5.948

Review 2.  Molecular techniques for diagnosis of Clostridium difficile infection: systematic review and meta-analysis.

Authors:  John C O'Horo; Amy Jones; Matthew Sternke; Christopher Harper; Nasia Safdar
Journal:  Mayo Clin Proc       Date:  2012-07       Impact factor: 7.616

3.  Assessment of Clostridium difficile infections by quantitative detection of tcdB toxin by use of a real-time cell analysis system.

Authors:  Alex B Ryder; Ying Huang; Haijing Li; Min Zheng; Xiaobo Wang; Charles W Stratton; Xiao Xu; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2010-08-18       Impact factor: 5.948

4.  Toxin-producing Clostridium difficile strains as long-term gut colonizers in healthy infants.

Authors:  Ingegerd Adlerberth; Haihui Huang; Erika Lindberg; Nils Åberg; Bill Hesselmar; Robert Saalman; Carl Erik Nord; Agnes E Wold; Andrej Weintraub
Journal:  J Clin Microbiol       Date:  2013-10-30       Impact factor: 5.948

Review 5.  Laboratory diagnosis of Clostridium difficile infection can molecular amplification methods move us out of uncertainty?

Authors:  Fred C Tenover; Ellen Jo Baron; Lance R Peterson; David H Persing
Journal:  J Mol Diagn       Date:  2011-08-18       Impact factor: 5.568

6.  Presumptive identification of Clostridium difficile strain 027/NAP1/BI on Cepheid Xpert: interpret with caution.

Authors:  Jen Kok; Qinning Wang; Lee C Thomas; Gwendolyn L Gilbert
Journal:  J Clin Microbiol       Date:  2011-08-17       Impact factor: 5.948

7.  Characteristics and Antibiotic Use Associated With Short-Term Risk of Clostridium difficile Infection Among Hospitalized Patients.

Authors:  Sol del Mar Aldrete; Matthew J Magee; Rachel J Friedman-Moraco; Austin W Chan; Grier G Banks; Eileen M Burd; Colleen S Kraft
Journal:  Am J Clin Pathol       Date:  2015-06       Impact factor: 2.493

8.  Clinical characteristics of patients who test positive for Clostridium difficile by repeat PCR.

Authors:  Daniel A Green; Brie Stotler; Dana Jackman; Susan Whittier; Phyllis Della-Latta
Journal:  J Clin Microbiol       Date:  2014-08-13       Impact factor: 5.948

9.  Rapid diagnosis of Clostridium difficile infection by multiplex real-time PCR.

Authors:  F Barbut; M Monot; A Rousseau; S Cavelot; T Simon; B Burghoffer; V Lalande; J Tankovic; J-C Petit; B Dupuy; C Eckert
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-04-13       Impact factor: 3.267

10.  Methods for determining disease burden and calibrating national surveillance data in the United Kingdom: the second study of infectious intestinal disease in the community (IID2 study).

Authors:  Sarah J O'Brien; Greta Rait; Paul R Hunter; James J Gray; Frederick J Bolton; David S Tompkins; Jim McLauchlin; Louise H Letley; Goutam K Adak; John M Cowden; Meirion R Evans; Keith R Neal; Gillian E Smith; Brian Smyth; Clarence C Tam; Laura C Rodrigues
Journal:  BMC Med Res Methodol       Date:  2010-05-05       Impact factor: 4.615
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