William S Harris1, Rachael M Thomas. 1. Cardiovascular Health Research Center, Sanford Research/USD and Sanford School of Medicine, Sioux Falls, SD 57105, USA. harrisw@sanfordhealth.org
Abstract
OBJECTIVES: We conducted a pilot study to estimate the biological variability and effects of a prior meal on the omega-3 fatty acid (FA) content of 3 blood FA pools. DESIGN AND METHODS: We measured FA levels in red blood cells (RBCs), plasma and plasma phospholipids (PL) obtained from 20 healthy volunteers tested weekly over 6 weeks. RESULTS: The within-subject coefficients of variation were 4.1%+/-1.9%, 15.9%+/-6.4%, and 14.5%+/-8.4%, respectively (RBC vs. others, p<0.001). RBC omega-3 FA content had the lowest biological variability and was not altered in the fed state. CONCLUSIONS: From the perspective of variability and of the sample types tested, RBCs may be the preferred sample type for assessing omega-3 FA status. (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
OBJECTIVES: We conducted a pilot study to estimate the biological variability and effects of a prior meal on the omega-3 fatty acid (FA) content of 3 blood FA pools. DESIGN AND METHODS: We measured FA levels in red blood cells (RBCs), plasma and plasma phospholipids (PL) obtained from 20 healthy volunteers tested weekly over 6 weeks. RESULTS: The within-subject coefficients of variation were 4.1%+/-1.9%, 15.9%+/-6.4%, and 14.5%+/-8.4%, respectively (RBC vs. others, p<0.001). RBC omega-3 FA content had the lowest biological variability and was not altered in the fed state. CONCLUSIONS: From the perspective of variability and of the sample types tested, RBCs may be the preferred sample type for assessing omega-3 FA status. (c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
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