Literature DB >> 197274

Analysis of polyoma virus DNA replicative intermediates by agarose gel electrophoresis.

R F Martin.   

Abstract

Agarose gel electrophoresis has been used to fractionate polyoma virus DNA replicative intermediates (RI) according to maturity. Approximate electrophoretic mobility versus maturity relationships were obtained for both intact (supercoiled) and nicked (relaxed) RI. There was considerable overlap between the supercoiled and relaxed RI populations after electrophoretic fractionation. Intact RI could be recovered from preparative agarose gels for further analysis by centrifugation, electron microscopy, re-electrophoresis, or nuclease digestion.

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Year:  1977        PMID： 197274      PMCID： PMC515896     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  19 in total

1.  Replication of polyoma DNA: effects of hydroxyurea and arabinosyl cytosine.

Authors:  G Magnusson; R Craig; M Närkhammar; P Reichard; M Staub; H Warner
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1975

2.  Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication.

Authors:  B Otto; P Reichard
Journal:  J Virol       Date:  1975-02       Impact factor: 5.103

3.  Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. I. Characterization of fragments.

Authors:  G Magnusson
Journal:  J Virol       Date:  1973-09       Impact factor: 5.103

4.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

5.  Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells.

Authors:  E L Winnacker; G Magnusson; P Reichard
Journal:  J Mol Biol       Date:  1972-12-30       Impact factor: 5.469

6.  Unwinding of replicating polyoma virus DNA.

Authors:  P Bourgaux; D Bourgaux-Ramoisy
Journal:  J Mol Biol       Date:  1972-10-14       Impact factor: 5.469

7.  Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors:  B Hirt
Journal:  J Mol Biol       Date:  1967-06-14       Impact factor: 5.469

8.  Simian virus 40 deoxyribonucleic acid synthesis: analysis by gel electrophoresis.

Authors:  P Tegtmeyer; F Macasaet
Journal:  J Virol       Date:  1972-10       Impact factor: 5.103

9.  RNA-linked short DNA fragments during polyoma replication.

Authors:  G Magnusson; V Pigiet; E L Winnacker; R Abrams; P Reichard
Journal:  Proc Natl Acad Sci U S A       Date:  1973-02       Impact factor: 11.205

10.  Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.

Authors:  U Pettersson; C Mulder; H Deluis; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1973-01       Impact factor: 11.205
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  5 in total

1.  Isolation and characterization of replication forks from discrete regions of the polyoma genome.

Authors:  A J Buckler-White; V Pigiet
Journal:  J Virol       Date:  1982-11       Impact factor: 5.103

2.  Polyoma virus minichromosomes: a soluble in vitro replication system.

Authors:  B B Gourlie; M R Krauss; A J Buckler-White; R M Benbow; V Pigiet
Journal:  J Virol       Date:  1981-06       Impact factor: 5.103

3.  Synthesis of multimeric polyoma virus DNA in mouse L-cells: role of the tsA1S9 gene product.

Authors:  P R Ganz; R Sheinin
Journal:  J Virol       Date:  1983-06       Impact factor: 5.103

4.  Distribution of replicating simian virus 40 DNA in intact cells and its maturation in isolated nuclei.

Authors:  D P Tapper; S Anderson; M L DePamphilis
Journal:  J Virol       Date:  1982-03       Impact factor: 5.103

5.  Polyoma virus minichromosomes: characterization of the products of in vitro DNA synthesis.

Authors:  B B Gourlie; V P Pigiet
Journal:  J Virol       Date:  1983-02       Impact factor: 5.103
  5 in total

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