OBJECTIVE: To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. METHODS: Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray. RESULTS: Of the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes. CONCLUSION: Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.
OBJECTIVE: To detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray. METHODS: Four wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray. RESULTS: Of the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes. CONCLUSION:Oligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.
Authors: Yvonne Linger; Alexander Kukhtin; Julia Golova; Alexander Perov; Peter Qu; Christopher Knickerbocker; Christopher G Cooney; Darrell P Chandler Journal: J Vis Exp Date: 2014-04-25 Impact factor: 1.355
Authors: Yvonne Linger; Christopher Knickerbocker; David Sipes; Julia Golova; Molly Franke; Roger Calderon; Leonid Lecca; Nitu Thakore; Rebecca Holmberg; Peter Qu; Alexander Kukhtin; Megan B Murray; Christopher G Cooney; Darrell P Chandler Journal: J Clin Microbiol Date: 2018-02-22 Impact factor: 5.948