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Literature DB >> 19718031

Somatic cell nuclear transfer in zebrafish.

Kannika Siripattarapravat¹, Boonya Pinmee, Patrick J Venta, Chia-Cheng Chang, Jose B Cibelli.

Abstract

We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.

Entities: Disease Species

Mesh：

Year: 2009 PMID： 19718031 DOI： 10.1038/nmeth.1369

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

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Authors: Rebecca L Lamason; Manzoor-Ali P K Mohideen; Jason R Mest; Andrew C Wong; Heather L Norton; Michele C Aros; Michael J Jurynec; Xianyun Mao; Vanessa R Humphreville; Jasper E Humbert; Soniya Sinha; Jessica L Moore; Pudur Jagadeeswaran; Wei Zhao; Gang Ning; Izabela Makalowska; Paul M McKeigue; David O'donnell; Rick Kittles; Esteban J Parra; Nancy J Mangini; David J Grunwald; Mark D Shriver; Victor A Canfield; Keith C Cheng
Journal: Science Date: 2005-12-16 Impact factor: 47.728

10. Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like.

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15 in total

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3. Efficient activation of reconstructed rat embryos by cyclin-dependent kinase inhibitors.

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4. Critical developmental stages for the efficiency of somatic cell nuclear transfer in zebrafish.

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Review 7. Fish stem cell cultures.

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