OBJECTIVE: The purpose of this in vitro study was to evaluate the dentine root surface roughness and the adherence of Streptococcus sanguinis (ATCC 10556) after treatment with an ultrasonic system, Er:YAG laser, or manual curette. BACKGROUND DATA: Bacterial adhesion and formation of dental biofilm after scaling and root planing may be a challenge to the long-term stability of periodontal therapy. MATERIALS AND METHODS: Forty flattened bovine roots were randomly assigned to one of the following groups: ultrasonic system (n = 10); Er:YAG laser (n = 10); manual curette (n = 10); or control untreated roots (n = 10). The mean surface roughness (Ra, microm) of the specimens before and after exposure to each treatment was determined using a surface profilometer. In addition, S. sanguinis was grown on the treated and untreated specimens and the amounts of retained bacteria on the surfaces were measured by culture method. RESULTS: All treatments increased the Ra; however, the roughest surface was produced by the curettes. In addition, the specimens treated with curettes showed the highest S. sanguinis adhesion. There was a significant positive correlation between roughness values and bacterial cells counts. CONCLUSION: S. sanguinis adhesion was the highest on the curette-treated dentine root surfaces, which also presented the greatest surface roughness.
OBJECTIVE: The purpose of this in vitro study was to evaluate the dentine root surface roughness and the adherence of Streptococcus sanguinis (ATCC 10556) after treatment with an ultrasonic system, Er:YAG laser, or manual curette. BACKGROUND DATA: Bacterial adhesion and formation of dental biofilm after scaling and root planing may be a challenge to the long-term stability of periodontal therapy. MATERIALS AND METHODS: Forty flattened bovine roots were randomly assigned to one of the following groups: ultrasonic system (n = 10); Er:YAG laser (n = 10); manual curette (n = 10); or control untreated roots (n = 10). The mean surface roughness (Ra, microm) of the specimens before and after exposure to each treatment was determined using a surface profilometer. In addition, S. sanguinis was grown on the treated and untreated specimens and the amounts of retained bacteria on the surfaces were measured by culture method. RESULTS: All treatments increased the Ra; however, the roughest surface was produced by the curettes. In addition, the specimens treated with curettes showed the highest S. sanguinis adhesion. There was a significant positive correlation between roughness values and bacterial cells counts. CONCLUSION:S. sanguinis adhesion was the highest on the curette-treated dentine root surfaces, which also presented the greatest surface roughness.
Authors: Frank Schwarz; Anton Sculean; Mohammad Berakdar; Thomas Georg; Elmar Reich; Jürgen Becker Journal: J Periodontol Date: 2003-05 Impact factor: 6.993
Authors: C L Drisko; D L Cochran; T Blieden; O J Bouwsma; R E Cohen; P Damoulis; J B Fine; G Greenstein; J Hinrichs; M J Somerman; V Iacono; R J Genco Journal: J Periodontol Date: 2000-11 Impact factor: 6.993
Authors: Iliria S Feist; Giorgio De Micheli; Silvia R S Carneiro; Carlos P Eduardo; Sueli Miyagi; Márcia M Marques Journal: J Periodontol Date: 2003-09 Impact factor: 6.993