PURPOSE: This study examines the levels of oxidative damage in patients with cataract. METHODS: Blood samples were collected from 60 patients with cataract and 60 age- and gender-matched healthy individuals to measure 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels. RESULTS: A significant difference was observed in leukocyte 8-OHdG levels in patients with cataract in comparison with healthy persons (p < 0.001). Similarly, a significant difference was observed in plasma MDA levels in patients with cataract in comparison with healthy persons (p<0.001). In addition, a significant correlation was found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r = 0.859, p < 0.001). CONCLUSION: This study measured the oxidative DNA damage by measuring the 8-OHdG in the leukocyte DNA in patients with cataract. In addition, the level of MDA - a marker for lipid peroxidation - was measured to determine lipid peroxidation.
PURPOSE: This study examines the levels of oxidative damage in patients with cataract. METHODS: Blood samples were collected from 60 patients with cataract and 60 age- and gender-matched healthy individuals to measure 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels. RESULTS: A significant difference was observed in leukocyte 8-OHdG levels in patients with cataract in comparison with healthy persons (p < 0.001). Similarly, a significant difference was observed in plasma MDA levels in patients with cataract in comparison with healthy persons (p<0.001). In addition, a significant correlation was found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r = 0.859, p < 0.001). CONCLUSION: This study measured the oxidative DNA damage by measuring the 8-OHdG in the leukocyte DNA in patients with cataract. In addition, the level of MDA - a marker for lipid peroxidation - was measured to determine lipid peroxidation.
Authors: Mark L Wang; C Edward Hoffler; Asif M Ilyas; William H Kirkpatrick; Pedro K Beredjiklian; Charles F Leinberry Journal: Hand (N Y) Date: 2016-04-06
Authors: Caroline S Cencer; Shravan K Chintala; Tenira J Townsend; Daniel P Feldmann; Mirna A Awrow; Nahrain A Putris; Mason E Geno; Maria G Donovan; Frank J Giblin Journal: Photochem Photobiol Date: 2017-09-15 Impact factor: 3.421
Authors: Shepherd H Schurman; Christopher A Dunn; Rebecca Greaves; Binbing Yu; Luigi Ferrucci; Deborah L Croteau; Michael M Seidman; Vilhelm A Bohr Journal: PLoS One Date: 2012-09-21 Impact factor: 3.240