| Literature DB >> 19697313 |
Zhongduo Yang1, Xu Zhang, Dongzhu Duan, Zhuwen Song, Mingjun Yang, Shuo Li.
Abstract
The present work describes modifications to an existing TLC bioautographic method for detecting acetylcholinesterase inhibitors from plant extracts. The basic principle of the method is that the enzyme converts 1-naphthyl acetate into naphthol which reacts with Fast Blue B salt to make a purple-colored background on the TLC plates. Inhibitors of acetylcholinesterases produced white spots on the background. Our modifications involve changes in the concentration of the enzyme, the reagents, and the time of the reaction. With these changes, the consumption of the enzyme was reduced by 85% and the detection limits were decreased remarkably.Entities:
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Year: 2009 PMID： 19697313 DOI： 10.1002/jssc.200900266
Source DB: PubMed Journal: J Sep Sci ISSN： 1615-9306 Impact factor: 3.645