BACKGROUND: The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. METHODS: DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. RESULTS: DNA-associated HLA-G(+) MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p<0.005), with highest levels found in the first and second trimesters. DNA-associated PLAP(+) MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p<0.006), with highest levels in the second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G(+) MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G(+) MPs from the plasma of normal pregnant women (p<0.03). CONCLUSIONS: HLA-G(+) and PLAP(+) MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G(+) MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.
BACKGROUND: The aim of this study was to determine whether DNA-associated micro-particles (MPs) in maternal plasma express fetal-derived human leukocyte antigen-G (HLA-G) or placental alkaline phosphatase (PLAP) and whether the levels differ between women with normotensive pregnancies and preeclampsia. METHODS: DNA-associated MPs expressing HLA-G or PLAP were examined in the plasma of normal pregnant women and preeclamptic patients using flow cytometric analysis. RESULTS: DNA-associated HLA-G(+) MPs were significantly increased in maternal plasma compared to plasma from non-pregnant controls (p<0.005), with highest levels found in the first and second trimesters. DNA-associated PLAP(+) MPs were also increased in maternal plasma compared to plasma from non-pregnant controls (p<0.006), with highest levels in the second and third trimesters. Term preeclamptic women had higher levels of DNA-associated MPs than control pregnant women. HLA-G(+) MPs from the plasma of preeclamptic women had more DNA per MP than HLA-G(+) MPs from the plasma of normal pregnant women (p<0.03). CONCLUSIONS:HLA-G(+) and PLAP(+) MPs increase in maternal circulation at different times during gestation. DNA amounts per HLA-G(+) MP increase in preeclamptic women which might indicate dysfunctional extravillous cytotrophoblasts.
Authors: Aaron F Orozco; Carolina J Jorgez; Cassandra Horne; Deborah A Marquez-Do; Matthew R Chapman; John R Rodgers; Farideh Z Bischoff; Dorothy E Lewis Journal: Am J Pathol Date: 2008-10-30 Impact factor: 4.307
Authors: Christine A R Lok; Anita N Böing; Ian L Sargent; Suren R Sooranna; Joris A M van der Post; Rienk Nieuwland; Augueste Sturk Journal: Reprod Sci Date: 2008-10-20 Impact factor: 3.060
Authors: Christine A R Lok; Joris A M Van Der Post; Ian L Sargent; Chi M Hau; Augueste Sturk; Kees Boer; Rienk Nieuwland Journal: Hypertens Pregnancy Date: 2008 Impact factor: 2.108
Authors: Roberta Rizzo; Martine Vercammen; Hilde van de Velde; Peter A Horn; Vera Rebmann Journal: Cell Mol Life Sci Date: 2010-11-16 Impact factor: 9.261