BACKGROUND: Flt-1 is secreted by various cells and elevated concentrations are present in preeclampsia affecting vascular function. Microparticles from these cells may expose Flt-1. We evaluated whether Flt-1 is microparticle-associated in preeclampsia, and established the origin of Flt-1-exposing microparticles. METHODS: The concentration of Flt-1 was measured in samples from preeclamptic patients, pregnant and nonpregnant women by enzyme-linked immunosorbent assay. Microparticles were analyzed by flow cytometry. Western blot determined the different forms of Flt-1. RESULTS: Noncell bound Flt-1 was elevated in preeclampsia compared to controls. A fraction (5%) was associated with microparticles in preeclampsia. Flt-1-exposing microparticles were increased in preeclampsia compared to normotensive pregnancy (p = 0.02). Full-length Flt-1, was identified in microparticles of platelet and placental origin. CONCLUSION: Full-length Flt-1 is associated with platelet and placenta-derived microparticles. Possibly, the presentation of Flt-1 on the membrane of a microparticle might alter its function, particularly if it acts in synergism with other exposed vasoactive molecules.
BACKGROUND:Flt-1 is secreted by various cells and elevated concentrations are present in preeclampsia affecting vascular function. Microparticles from these cells may expose Flt-1. We evaluated whether Flt-1 is microparticle-associated in preeclampsia, and established the origin of Flt-1-exposing microparticles. METHODS: The concentration of Flt-1 was measured in samples from preeclamptic patients, pregnant and nonpregnant women by enzyme-linked immunosorbent assay. Microparticles were analyzed by flow cytometry. Western blot determined the different forms of Flt-1. RESULTS: Noncell bound Flt-1 was elevated in preeclampsia compared to controls. A fraction (5%) was associated with microparticles in preeclampsia. Flt-1-exposing microparticles were increased in preeclampsia compared to normotensive pregnancy (p = 0.02). Full-length Flt-1, was identified in microparticles of platelet and placental origin. CONCLUSION: Full-length Flt-1 is associated with platelet and placenta-derived microparticles. Possibly, the presentation of Flt-1 on the membrane of a microparticle might alter its function, particularly if it acts in synergism with other exposed vasoactive molecules.
Authors: Beth S Holder; Clare L Tower; Karen Forbes; Melissa J Mulla; John D Aplin; Vikki M Abrahams Journal: Immunology Date: 2012-06 Impact factor: 7.397
Authors: Carlos Palma; Jessica Jellins; Andrew Lai; Alexis Salas; America Campos; Shayna Sharma; Gregory Duncombe; Jon Hyett; Carlos Salomon Journal: Subcell Biochem Date: 2021
Authors: A F Orozco; C J Jorgez; W D Ramos-Perez; E J Popek; X Yu; C A Kozinetz; F Z Bischoff; D E Lewis Journal: Placenta Date: 2009-08-18 Impact factor: 3.481
Authors: Chris Gardiner; Dionne S Tannetta; Carol A Simms; Paul Harrison; Christopher W G Redman; Ian L Sargent Journal: PLoS One Date: 2011-10-14 Impact factor: 3.240