BACKGROUND: Infection with multiple human papillomavirus (HPV) types is common. However, it is unknown whether viral DNA load is related to the coexistence of other types. METHODS: Study subjects were 802 and 303 women who were positive for HPV16 and HPV18, respectively, at enrollment into the Atypical Squamous Cells of Undetermined Significance and Low-Grade Squamous Intraepithelial Lesion Triage Study. HPV16 and HPV18 E7 copies per nanogram of cellular DNA in cervical swab samples were measured by real-time PCR in triplicate. RESULTS: Concurrent coinfection was common in this population of women with minor cervical lesions; multiple HPV types were detected in 573 (71.4%) of 802 HPV16-positive women and 227 (74.9%) of 303 HPV18-positive women. The adjusted odds ratio associating coinfection with per 1 log unit increase in HPV16 DNA load was 0.78 (95% confidence interval, 0.68-0.89); it was 0.64 (95% confidence interval, 0.52-0.79) for a similar analysis of HPV18 DNA load. Women with, compared with without, coinfection of A9 species types possessed a significantly lower HPV16 DNA load (P < 0.001), whereas women with, compared with without, coinfection of A7 species types possessed a significantly lower HPV18 DNA load (P = 0.001). A trend of decrease in HPV16 DNA load with increasing number of the coexisting non-HPV16 A9 species types was statistically significant (P(trend) = 0.001). CONCLUSION: Coinfection with other types was associated with lower HPV16 and HPV18 DNA load. The extent of reduction was correlated to phylogenetic distance of the coexisting types to HPV16 and HPV18, respectively.
BACKGROUND:Infection with multiple human papillomavirus (HPV) types is common. However, it is unknown whether viral DNA load is related to the coexistence of other types. METHODS: Study subjects were 802 and 303 women who were positive for HPV16 and HPV18, respectively, at enrollment into the Atypical Squamous Cells of Undetermined Significance and Low-Grade Squamous Intraepithelial Lesion Triage Study. HPV16 and HPV18 E7 copies per nanogram of cellular DNA in cervical swab samples were measured by real-time PCR in triplicate. RESULTS: Concurrent coinfection was common in this population of women with minor cervical lesions; multiple HPV types were detected in 573 (71.4%) of 802 HPV16-positive women and 227 (74.9%) of 303 HPV18-positive women. The adjusted odds ratio associating coinfection with per 1 log unit increase in HPV16 DNA load was 0.78 (95% confidence interval, 0.68-0.89); it was 0.64 (95% confidence interval, 0.52-0.79) for a similar analysis of HPV18 DNA load. Women with, compared with without, coinfection of A9 species types possessed a significantly lower HPV16 DNA load (P < 0.001), whereas women with, compared with without, coinfection of A7 species types possessed a significantly lower HPV18 DNA load (P = 0.001). A trend of decrease in HPV16 DNA load with increasing number of the coexisting non-HPV16 A9 species types was statistically significant (P(trend) = 0.001). CONCLUSION: Coinfection with other types was associated with lower HPV16 and HPV18 DNA load. The extent of reduction was correlated to phylogenetic distance of the coexisting types to HPV16 and HPV18, respectively.
Authors: Nicolas F Schlecht; Andrea Trevisan; Eliane Duarte-Franco; Thomas E Rohan; Alex Ferenczy; Luisa L Villa; Eduardo L Franco Journal: Int J Cancer Date: 2003-02-10 Impact factor: 7.396
Authors: Alba-Lucia Combita; Maria-Mercedes Bravo; Antoine Touzé; Oscar Orozco; Pierre Coursaget Journal: Int J Cancer Date: 2002-02-20 Impact factor: 7.396
Authors: Ligia A Pinto; Jessica Edwards; Philip E Castle; Clayton D Harro; Douglas R Lowy; John T Schiller; Dora Wallace; William Kopp; Joseph W Adelsberger; Michael W Baseler; Jay A Berzofsky; Allan Hildesheim Journal: J Infect Dis Date: 2003-07-09 Impact factor: 5.226
Authors: Attila T Lorincz; Philip E Castle; Mark E Sherman; David R Scott; Andrew G Glass; Sholom Wacholder; Brenda B Rush; Patti E Gravitt; John E Schussler; Mark Schiffman Journal: Lancet Date: 2002-07-20 Impact factor: 79.321
Authors: Mark E Sherman; Sophia S Wang; Cosette M Wheeler; Laurie Rich; Patti E Gravitt; Robert Tarone; Mark Schiffman Journal: Cancer Epidemiol Biomarkers Prev Date: 2003-10 Impact factor: 4.254
Authors: Alba-Lucia Combita; Antoine Touzé; Latifa Bousarghin; Neil D Christensen; Pierre Coursaget Journal: J Virol Date: 2002-07 Impact factor: 5.103
Authors: Ethel-Michele de Villiers; Claude Fauquet; Thomas R Broker; Hans-Ulrich Bernard; Harald zur Hausen Journal: Virology Date: 2004-06-20 Impact factor: 3.616
Authors: S S Wang; M Schiffman; T S Shields; R Herrero; A Hildesheim; M C Bratti; M E Sherman; A C Rodriguez; P E Castle; J Morales; M Alfaro; T Wright; S Chen; B Clayman; R D Burk; R P Viscidi Journal: Br J Cancer Date: 2003-10-06 Impact factor: 7.640
Authors: Anil K Chaturvedi; Hormuzd A Katki; Allan Hildesheim; Ana Cecilia Rodríguez; Wim Quint; Mark Schiffman; Leen-Jan Van Doorn; Carolina Porras; Sholom Wacholder; Paula Gonzalez; Mark E Sherman; Rolando Herrero Journal: J Infect Dis Date: 2011-04-01 Impact factor: 5.226
Authors: Irene Man; Kari Auranen; Jacco Wallinga; Johannes A Bogaards Journal: Philos Trans R Soc Lond B Biol Sci Date: 2019-05-27 Impact factor: 6.237
Authors: Long Fu Xi; Laura A Koutsky; Philip E Castle; Zoe R Edelstein; Craig Meyers; Jesse Ho; Mark Schiffman Journal: Cancer Epidemiol Biomarkers Prev Date: 2009-12 Impact factor: 4.254
Authors: Long Fu Xi; James P Hughes; Zoe R Edelstein; Nancy B Kiviat; Laura A Koutsky; Constance Mao; Jesse Ho; Mark Schiffman Journal: J Infect Dis Date: 2009-12-01 Impact factor: 5.226
Authors: L Del Río-Ospina; S C Soto-DE León; M Camargo; R Sánchez; D A Moreno-Pérez; A Pérez-Prados; M E Patarroyo; M A Patarroyo Journal: Epidemiol Infect Date: 2017-02-10 Impact factor: 4.434