Literature DB >> 19685327

Analysis of chromosome dynamics and chromosomal proteins in Drosophila spermatocytes.

Sharon E Thomas1, Bruce D McKee.   

Abstract

A wide variety of techniques have been utilized to determine the localization of various proteins from premeiotic through meiotic stages in Drosophila males. Live imaging has been instrumental in monitoring chromosome pairing and the localization of fusion proteins. Immunofluorescence has been a widely utilized technique to examine the localization and colocalization of the many proteins involved in meiosis. Recently, an immuno-FISH protocol was developed to observe the co-localization of DNA probes and proteins. In this chapter, detailed protocols outlining these three types of experiments are presented.

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Year:  2009        PMID： 19685327     DOI： 10.1007/978-1-60761-103-5_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Homolog pairing and sister chromatid cohesion in heterochromatin in Drosophila male meiosis I.

Authors:  Jui-He Tsai; Rihui Yan; Bruce D McKee
Journal:  Chromosoma       Date:  2011-03-08       Impact factor: 4.316

2.  MNM and SNM maintain but do not establish achiasmate homolog conjunction during Drosophila male meiosis.

Authors:  Michael Shoujie Sun; Joe Weber; Ariane C Blattner; Soumya Chaurasia; Christian F Lehner
Journal:  PLoS Genet       Date:  2019-05-28       Impact factor: 5.917

3.  Dynamic sex chromosome expression in Drosophila male germ cells.

Authors:  Sharvani Mahadevaraju; Justin M Fear; Miriam Akeju; Brian J Galletta; Mara M L S Pinheiro; Camila C Avelino; Diogo C Cabral-de-Mello; Katie Conlon; Stafania Dell'Orso; Zelalem Demere; Kush Mansuria; Carolina A Mendonça; Octavio M Palacios-Gimenez; Eli Ross; Max Savery; Kevin Yu; Harold E Smith; Vittorio Sartorelli; Haiwang Yang; Nasser M Rusan; Maria D Vibranovski; Erika Matunis; Brian Oliver
Journal:  Nat Commun       Date:  2021-02-09       Impact factor: 14.919

4.  Comparison of genome architecture at two stages of male germline cell differentiation in Drosophila.

Authors:  Artem A Ilyin; Anna D Kononkova; Anastasia V Golova; Viktor V Shloma; Oxana M Olenkina; Valentina V Nenasheva; Yuri A Abramov; Alexei A Kotov; Daniil A Maksimov; Petr P Laktionov; Alexey V Pindyurin; Aleksandra A Galitsyna; Sergey V Ulianov; Ekaterina E Khrameeva; Mikhail S Gelfand; Stepan N Belyakin; Sergey V Razin; Yuri Y Shevelyov
Journal:  Nucleic Acids Res       Date:  2022-04-08       Impact factor: 16.971

5.  Separase Is Required for Homolog and Sister Disjunction during Drosophila melanogaster Male Meiosis, but Not for Biorientation of Sister Centromeres.

Authors:  Ariane C Blattner; Soumya Chaurasia; Bruce D McKee; Christian F Lehner
Journal:  PLoS Genet       Date:  2016-04-27       Impact factor: 5.917
  5 in total

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