| Literature DB >> 33563972 |
Sharvani Mahadevaraju1, Justin M Fear1, Miriam Akeju2, Brian J Galletta3, Mara M L S Pinheiro4, Camila C Avelino4, Diogo C Cabral-de-Mello5, Katie Conlon2, Stafania Dell'Orso6, Zelalem Demere2, Kush Mansuria2, Carolina A Mendonça4, Octavio M Palacios-Gimenez4,7, Eli Ross2, Max Savery1, Kevin Yu2, Harold E Smith8, Vittorio Sartorelli6, Haiwang Yang1,9, Nasser M Rusan3, Maria D Vibranovski4, Erika Matunis2, Brian Oliver10.
Abstract
Given their copy number differences and unique modes of inheritance, the evolved gene content and expression of sex chromosomes is unusual. In many organisms the X and Y chromosomes are inactivated in spermatocytes, possibly as a defense mechanism against insertions into unpaired chromatin. In addition to current sex chromosomes, Drosophila has a small gene-poor X-chromosome relic (4th) that re-acquired autosomal status. Here we use single cell RNA-Seq on fly larvae to demonstrate that the single X and pair of 4th chromosomes are specifically inactivated in primary spermatocytes, based on measuring all genes or a set of broadly expressed genes in testis we identified. In contrast, genes on the single Y chromosome become maximally active in primary spermatocytes. Reduced X transcript levels are due to failed activation of RNA-Polymerase-II by phosphorylation of Serine 2 and 5.Entities:
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Year: 2021 PMID： 33563972 PMCID： PMC7873209 DOI： 10.1038/s41467-021-20897-y
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919