Brevundimonas basaltis sp. nov., isolated from black sand.

A Gram-negative, aerobic, rod-shaped, motile Brevundimonas-like bacterial strain, J22(T), was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Growth of strain J22(T) was observed in R2A medium at temperatures between 10 and 42 degrees C (optimum 30 degrees C), between pH 6.5 and 10.5 (optimum pH 7.5) and at a NaCl concentration between 0 and 4 % (w/v) (optimum 0-1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J22(T) belonged to the genus Brevundimonas, with high sequence similarities of >97 % to the sequence of the type strains Brevundimonas alba CB88(T), Brevundimonas lenta DS-18(T), Brevundimonas variabilis CB17(T), Mycoplana bullata TK0051(T), Brevundimonas kwangchunensis KSL-102(T), Brevundimonas intermedia CB63(T), Brevundimonas subvibrioides CB81(T) and Brevundimonas bacteroides CB7(T). Strain J22(T) exhibited DNA-DNA relatedness values of less than 22.2 % with the phylogenetically related species of the genus Brevundimonas. The DNA G+C content of strain J22(T) was 66.3 mol%. The predominant cellular fatty acids were C(18 : 1)omega7c, C(16 : 0) and C(16 : 1)omega9c; C(12 : 0) 3-OH was present, which chemotaxonomically characterizes the members of the genus Brevundimonas. Phylogenetic, genomic and biochemical characteristics served to differentiate this isolate from recognized members of the genus Brevundimonas. Strain J22(T) (=KCTC 22177(T)=JCM 15911(T)) should be classified as a novel species in the genus Brevundimonas, for which the name Brevundimonas basaltis sp. nov. is proposed.