BACKGROUND: The early diagnosis of tuberculous meningitis (TBM) is very crucial, since delayed diagnosis can lead to various neurological manifestations. We have previously developed an in-house indirect enzyme-linked immunosorbent assay (ELISA) for TBM diagnosis using the Antigen 85 (Ag 85) complex. It has been suggested that the Ag 85 complex might give false-positive reactions for individuals vaccinated with Bacillus Calmette-Guérin (BCG). OBJECTIVES: In the present study, we describe a prospective evaluation demonstrating that early secreted antigenic target- 6 (ESAT-6), which is absent in Mycobacterium bovis BCG strains, is in the cerebrospinal fluid (CSF) of TBM patients. METHODS: We used an indirect ELISA to detect ESAT-6 antigens in the CSF of TBM patients using polyclonal antibodies against ESAT-6. RESULTS: Using the indirect ELISA method, we demonstrated a sensitivity and specificity of 80% and 94%, respectively, for the diagnosis of TBM. CONCLUSION: The detection of ESAT-6 in the CSF of TBM patients by indirect ELISA is a promising method and can be used to develop an immunodiagnostic assay with increased sensitivity and specificity.
BACKGROUND: The early diagnosis of tuberculous meningitis (TBM) is very crucial, since delayed diagnosis can lead to various neurological manifestations. We have previously developed an in-house indirect enzyme-linked immunosorbent assay (ELISA) for TBM diagnosis using the Antigen 85 (Ag 85) complex. It has been suggested that the Ag 85 complex might give false-positive reactions for individuals vaccinated with Bacillus Calmette-Guérin (BCG). OBJECTIVES: In the present study, we describe a prospective evaluation demonstrating that early secreted antigenic target- 6 (ESAT-6), which is absent in Mycobacterium bovis BCG strains, is in the cerebrospinal fluid (CSF) of TBM patients. METHODS: We used an indirect ELISA to detect ESAT-6 antigens in the CSF of TBM patients using polyclonal antibodies against ESAT-6. RESULTS: Using the indirect ELISA method, we demonstrated a sensitivity and specificity of 80% and 94%, respectively, for the diagnosis of TBM. CONCLUSION: The detection of ESAT-6 in the CSF of TBM patients by indirect ELISA is a promising method and can be used to develop an immunodiagnostic assay with increased sensitivity and specificity.
Authors: Rajpal S Kashyap; Neha P Agarwal; Nitin H Chandak; Girdhar M Taori; Saibal K Biswas; Hemant J Purohit; Hatim F Daginawala Journal: Med Sci Monit Date: 2002-06
Authors: M M Thomas; T S C Hinks; S Raghuraman; N Ramalingam; M Ernst; R Nau; C Lange; K Kösters; C Gnanamuthu; G T John; B Marshall; A Lalvani Journal: Int J Tuberc Lung Dis Date: 2008-06 Impact factor: 2.373
Authors: Poonam S Deshpande; Rajpal S Kashyap; Sonali S Ramteke; Khushboo J Nagdev; Hemant J Purohit; Girdhar M Taori; Hatim F Daginawala Journal: Cerebrospinal Fluid Res Date: 2007-11-02
Authors: Khushboo J Nagdev; Sharda P Bhagchandani; Shradha S Bhullar; Rajkumar C Kapgate; Rajpal S Kashyap; Nitin H Chandak; Hatim F Daginawala; Hemant J Purohit; Girdhar M Taori Journal: Indian J Microbiol Date: 2015-02-03 Impact factor: 2.461
Authors: Dewi Kartika Turbawaty; Adhi Kristianto Sugianli; Arto Yuwono Soeroto; Budi Setiabudiawan; Ida Parwati Journal: Int J Microbiol Date: 2017-10-18