BACKGROUND: Tuberculous meningitis (TBM) is still a serious cause of morbidity and mortality in developing nations, and the timing of treatment is the most crucial factor affecting the ultimate outcome. To establish a rapid diagnosis, we used Single Radial Immuno-Diffusion (SRID) to detect circulating mycobacterium antigen in the CSF of patients with clinically suspected TBM. MATERIAL/ METHODS: Single radial immunodiffusion is the simplest of all immunotechniques for the quantitative determination of antigen or antibody. CSF was collected by standard lumbar puncture. Antiserum was raised against CSF from clinically suspected TBM patients by standard Immunization procedures. RESULTS: The developed protocol was tested with 73 CSF samples collected over a period of one year. The assay gave 94% sensitivity for the detection of mycobacterium antigen in the CSF of patients with clinically suspected TBM. CONCLUSIONS: This study suggests that single radial immunodiffusion is useful for quantitative as well as qualitative determination of mycobacterium antigen. The developed technique is of potential value in the laboratory diagnosis of TBM.
BACKGROUND:Tuberculous meningitis (TBM) is still a serious cause of morbidity and mortality in developing nations, and the timing of treatment is the most crucial factor affecting the ultimate outcome. To establish a rapid diagnosis, we used Single Radial Immuno-Diffusion (SRID) to detect circulating mycobacterium antigen in the CSF of patients with clinically suspected TBM. MATERIAL/ METHODS: Single radial immunodiffusion is the simplest of all immunotechniques for the quantitative determination of antigen or antibody. CSF was collected by standard lumbar puncture. Antiserum was raised against CSF from clinically suspected TBM patients by standard Immunization procedures. RESULTS: The developed protocol was tested with 73 CSF samples collected over a period of one year. The assay gave 94% sensitivity for the detection of mycobacterium antigen in the CSF of patients with clinically suspected TBM. CONCLUSIONS: This study suggests that single radial immunodiffusion is useful for quantitative as well as qualitative determination of mycobacterium antigen. The developed technique is of potential value in the laboratory diagnosis of TBM.
Authors: Rajpal S Kashyap; Karen M Dobos; John T Belisle; Hemant J Purohit; Nitin H Chandak; Girdhar M Taori; Hatim F Daginawala Journal: Clin Diagn Lab Immunol Date: 2005-06
Authors: Rajpal S Kashyap; Rani P Kainthla; Anju V Mudaliar; Hemant J Purohit; Girdhar M Taori; Hatim F Daginawala Journal: Cerebrospinal Fluid Res Date: 2006-03-30
Authors: Rajpal S Kashyap; Rani P Kainthla; Ravindra M Satpute; Neha P Agarwal; Nitin H Chandak; Hemant J Purohit; Girdhar M Taori; Hatim F Daginawala Journal: BMC Neurol Date: 2004-10-22 Impact factor: 2.474
Authors: Rajpal S Kashyap; Anju N Rajan; Sonali S Ramteke; Vijay S Agrawal; Sanjivani S Kelkar; Hemant J Purohit; Girdhar M Taori; Hatim F Daginawala Journal: BMC Infect Dis Date: 2007-07-10 Impact factor: 3.090