Using basic science to design a clinical trial: baseline characteristics of women enrolled in the Kronos Early Estrogen Prevention Study (KEEPS).

Observational and epidemiological studies suggest that menopausal hormone therapy (MHT) reduces cardiovascular disease (CVD) risk. However, results from prospective trials showed neutral or adverse effects most likely due to differences in participant demographics, such as age, timing of initiation of treatment, and preexisting cardiovascular disease, which reflected in part the lack of basic science information on mechanisms of action of hormones on the vasculature at the time clinical trials were designed. The Kronos Early Estrogen Replacement Study (KEEPS) is a prospective, randomized, controlled trial designed, using findings from basic science studies, to test the hypothesis that MHT when initiated early in menopause reduces progression of atherosclerosis. KEEPS participants are younger, healthier, and within 3 years of menopause thus matching more closely demographics of women in prior observational and epidemiological studies than women in the Women's Health Initiative hormone trials. KEEPS will provide information relevant to the critical timing hypothesis for MHT use in reducing risk for CVD.

RCT Entities:   Population Interventions Outcomes

Introduction

Chronic diseases potentially affected by menopausal hormone treatment (MHT) include cardiovascular disease (CVD; which includes all atherosclerotic disease such as coronary heart disease (CHD) and stroke), osteoporosis, venous thromboembolic disease and cancers of the breast, endometrium, and colon. These diseases constitute the most common sources of morbidity and mortality among women and cost scores of billions of dollars annually. Of these, CVD remains the single greatest killer of women, accounting for approximately 40% of total mortality vs. about 5% for breast cancer [62]. Over the last decade, controversies regarding benefits, risks, and appropriate use of MHT to prevent cardiovascular disease have flared when emerging clinical trial evidence appeared to be at odds with well-established observational and epidemiological data. This paper provides a brief review of the historical perspective behind the controversy surrounding the use and efficacy of MHT for prevention of CVD and highlights the design and demographics of an ongoing clinical trial designed to help resolve this controversy, in particular, as to the timing relative to menopause for initiation of treatment (critical timing hypothesis).

Historical Considerations

Human Studies Over the past 30 years, numerous large observational studies [9, 10, 24, 32, 34, 37, 84, 97] found that MHT, in addition to reducing menopausal symptoms, reduced both CVD incidence and all-cause mortality by nearly one half in postmenopausal women. Furthermore, MHT was reported in both observational studies and randomized clinical trials to significantly reduce the risk of osteoporosis-related fractures [12, 23, 65] and colon cancer [27] while increasing age-adjusted incidence of breast cancer by about 20% [6, 17, 76], but decreasing breast cancer mortality by over 20% [34]. Based on the expectation of CVD protection, the benefit/risk ratio for MHT was calculated to be positive for most women [16].The biological plausibility of atheroprevention by MHT is supported by a body of investigations demonstrating that estrogens improve a variety of known and suspected risk factors for atherosclerosis [44]. The Postmenopausal Estrogen/Progestin Interventions (PEPI) prospective trial, which compared the use of conjugated equine estrogen (CEE) alone and in three different CEE–progestin combinations and placebo in 875 healthy postmenopausal women aged 45–64 years, showed increases in high-density lipoprotein cholesterol (HDL-C) and decreases in low-density lipoprotein cholesterol (LDL-C) and fibrinogen in women receiving estrogen [91]. Subsequently, other studies have confirmed favorable lipid effects, including lowering of LDL-C and lipoprotein-a and raising of HDL-C levels [19, 28, 39, 53, 82].Estrogenic effects on lipid profiles alone, however, were considered unlikely to account for all of the evident beneficial effects of estrogen which appeared to account both for lower atherosclerosis rates in premenopausal women vs. age-matched men and for the exponential increase in cardiovascular risk in women associated with the menopause-related decline in estrogen. However, in the early 1990s when some of the results of these epidemiological and observational studies were published, little was known about potential nonlipid actions of estrogen on the cardiovascular system.However, recommendations were emerging from the observational studies and the PEPI trial for the use of MHT for women with coronary heart disease or at high risk for coronary heart disease [29]. Following on the heels of these recommendations, a major prospective clinical trial, the Women’s Health Initiative (WHI), was designed in 1991 and initiated in 1992 to test whether MHT would provide primary prevention for cardiovascular disease (i.e., reduction in the number of major cardiovascular events, heart attacks, and strokes). The selection of hormonal treatments (type and dose) for use in the WHI was based on the MHT regimens reported most frequently prescribed in the USA and which had been used in previous observational studies and the PEPI trial: conjugated equine estrogen (0.625 mg/day) plus medroxyprogesterone acetate (MPA; 2.5 mg/day), or CEE alone in women without a uterus [35, 91].In order to have sufficient statistical power to detect significant differences in cardiovascular event rates within the relatively short time frame of the trial, women across a wide spectrum of ages from 50 to 79 years with the vast majority of subjects older than 60 years were enrolled. In addition, a secondary end point in WHI was bone density for which there were few data available on effects of MHT in older women. In order to minimize dropouts among subjects randomized to placebo, women with severe menopausal symptoms who could not tolerate a 3-month washout period or might not tolerate randomization to placebo were excluded. Thus, for reasons of study practicality and statistical power, very few WHI subjects were like the women in the observational studies because observational data are based on clinical practice and in typical clinical practice, MHT was initiated early in the course of estrogen deficiency usually to treat menopausal symptoms. Occasionally, MHT was initiated years after the menopause. Women also were excluded from the WHI for prior CVD events but only by self-report. While these criteria were believed to be sufficient at the time of the trial, many subjects had significant CVD risk and/or were found to have had a prior CVD [35, 71].The CEE+MPA portion of the WHI trial was stopped early in 2002 because accumulating data showed a trend toward increased breast cancer which crossed a prespecified stopping point; thus, the benefit/risk ratio for the treatment was believed to have shifted from positive to negative [71]. Ironically, the effect on breast cancer was later found not to be statistically significant, and early termination of the trial rendered other findings similarly inconclusive. The CEE alone portion of the WHI was also terminated early, although this time for perceived lack of likelihood of ultimate net benefit, again truncating data collection and confounding interpretation of results. The key question remaining to be answered is “What are the reasons for the discrepancies in effects on cardiovascular protection among observational studies and this major clinical trial?”

Animal Studies Clues to the answer to this question come as a result of initiatives in the early 1990s from the National Institutes of Health that encouraged research into basic mechanisms of estrogen actions. The typical experimental animal model used to investigate arterial actions of estrogen was to remove ovaries from healthy adult animals. Following ovariectomy, estrogen, usually 17β-estradiol, was administered immediately or shortly after the procedure by way of intramuscular injection or subcutaneous time-release pellets or infusion minipumps. Thus, the timing of treatment was similar to that of clinical practice, that is, to initiate treatment around the time of estrogen deficiency.In support of this concept, estrogen treatment consistently reduced by 50–70% histologically defined coronary artery atherosclerosis if treatment was initiated immediately after ovariectomy in surgically menopausal (ovariectomized) cynomolgus monkeys, which develop atheromatous lesions similar to those in humans, but not if treatment was delayed for 2 years [14].In brief, these studies provided evidence to support potentially beneficial arterial actions of estrogen, as was suggested by observational studies in humans, including reduced formation of atheroma lesions at sites of endothelial injury, reduced blood pressure, and increased arterial compliance, all actions which maintain arterial health. Conversely, the absence of estrogen and/or related downregulation of estrogen receptors accelerates development of atherosclerosis [1, 15, 52, 86–89, 92, 93, 95, 98].

Rationale for the Design of the Kronos Early Estrogen Prevention Study

Thus, what has emerged from results of studies conducted in experimental animals and what can be identified as a critical difference between previous observational studies in humans and the WHI hormone trials was the time for initiation of treatment relative to menopause [43]. In particular, the mean age of menopause in the USA is 51 years, which corresponds to the age of women in the Nurses Health Study (NHS) who initiated MHT as in usual clinical practice for menopausal symptoms in the perimenopause [31]. This age and timing of MHT initiation contrasts with that of participants in the WHI who were an average of 63 years of age at MHT initiation, approximately 12 years past menopause [46, 59]. Because atherosclerosis progresses gradually before declaring itself with a clinical event [60, 64], a significant number of women in the WHI likely had subclinical atherosclerosis at randomization.

The presence of subclinical disease might have accounted for the tendency for CVD events to be increased early in the WHI, just as in the major secondary CVD prevention study of MHT, the Heart and Estrogen/Progestin Replacement Study (HERS) which began concurrently with the WHI. The HERS showed that in women with preexisting disease, there was an increase in CVD events in the first year or two of the trial. Although continued use of MHT in HERS was associated with a reversal of this trend, the major conclusion from that trial, contrary to conclusions drawn from meta-analyses of the literature [29] was that women with preexisting CHD were not candidates for preventive therapy with MHT [47]. This conclusion changed clinical practice.

Analysis of the NHS and post hoc analysis of data from the WHI based on timing of initiation of treatment relative to menopause supports the concept that initiation of treatment (up to 10 years after the menopause) reduces risk of adverse cardiac events [33, 46, 59]. Furthermore, as in the animal studies, MHT administered to individuals with established atherosclerosis did not induce regression of established lesions [11, 38, 42, 47]. Collectively, these studies suggest that MHT may provide primary prevention for CVD in women by preventing or reducing (slowing) early stages of atherogenic disease processes in the immediate time of menopause when endogenous estrogen shows the steepest decline [43]. Studies in humans, of course, were disadvantaged by the inability to obtain accurate histological assessment of disease progression, and noninvasive techniques to monitor progression of the atherosclerosis, such as B-mode ultrasound imaging of carotid artery wall thickness and plaque or computer-based tomography of coronary artery calcification, were not widely available nor were they sufficiently validated in 1991 to be used as endpoints when the WHI was designed. Advances in these technologies [30, 40] have prompted their use in a small subgroup of the CEE only group of the WHI at the end of the trial [58] and as the major endpoints in ongoing trials of MHT (see below).

An important difference between studies conducted in laboratory animals and clinical trials in humans is that most studies in animals, especially rodents, did not administer CEE but rather 17β-estradiol by a nonoral route. Therefore, possible differences between efficacy and safety of complex conjugated estrogen preparations and transdermal preparations of MHT on cardiovascular parameters remain to be clarified [22, 66, 74, 75, 77].

From these historical considerations, two hypotheses emerge which require rigorous testing in humans: (1) MHT initiated early after menopause (i.e., prior to the appearance of advanced atherosclerotic lesions) will prevent progression of atherosclerosis and (2) oral CEE and transdermal 17β-estradiol will be similarly efficacious in their arterial effects. These hypotheses are the basis of the Kronos Early Estrogen Prevention Study (KEEPS). Findings of the KEEPS have the potential to affect the health of many millions of women who may endure cardiac events and bone fractures that could be prevented. If there proves to be a window of time in the early postmenopause when initiation of long-term MHT has a net beneficial effect, then women can feel reassured that treatment of their menopausal symptoms when they are likely to be the most severe, can concurrently protect their vascular system, and can reduce bone resorption.

KEEPS Methods

KEEPS was designed as a randomized, placebo-controlled, double-blinded, prospective trial (KEEPS; NCT000154180) to evaluate effects of MHT on progression of atherosclerosis as defined by carotid intima–media thickness (CIMT) [44] and coronary arterial calcification (CAC) [8, 73] in women who more closely match the age of initiation of MHT reported by prior observational studies. Women meeting inclusion criteria subsequently were randomized to daily placebo, oral CEE, or transdermal 17β-estradiol with placebo or pulsed progesterone for 12 days/month. The detailed inclusion and exclusion criteria for KEEPS have been published elsewhere [36]. In brief, women between the ages of 42 and 58 years of age who were at least 6 months and no more than 36 months from their last menses with plasma follicle-stimulating hormone (FSH) level ≥35 ng/mL and/or E2 levels <40 pg/mL were eligible. A history of clinical CVD including myocardial infarction, angina, congestive heart failure, or thromboembolic disease excluded women from KEEPS. Other major cardiovascular risk factors excluding participation were current heavy smoking (more than ten cigarettes/day by self-report), morbid obesity [body mass index (BMI) >35 mm2/kg], dyslipidemia (LDL cholesterol >190 mg/dL), hypertriglyceridemia (triglycerides >400 mg/dL), and uncontrolled hypertension (systolic blood pressure >150 mm Hg and/or diastolic blood pressure >95 mm Hg) and glucose >126 mg/dL. Complete blood count and chemistry panel, estradiol, and FSH were performed at the clinical laboratories at each recruiting center. Lipid profiles and thyroid-stimulating hormone (TSH) were performed at the Kronos Science Laboratories (Phoenix, AZ, USA). At screening, women were asked to rank their menopausal symptoms (hotflashes, night sweats, vaginal dryness, dyspareunia, palpitations, insomnia, depression, mood swings, and irritability) as either none, mild, moderate, or severe. Finally, all subjects were screened for CAC and women with Agatston score ≥50 U, indicating significant subclinical coronary artery disease, were excluded. All women meeting inclusion criteria underwent baseline measurements of CIMT by B-mode ultrasound [44]. All imaging results are read centrally by individuals blinded to participant demographics (CAC at the Los Angeles Biomedical Research Institute at Harbor-UCLA, Torrance, CA, USA under the direction of Dr. M. Budoff and CIMT at the Atherosclerosis Research Unit Core Imaging and Reading Center, University of Southern California, Los Angeles, CA, USA under the direction of Dr. H. Hodis).

Analysis of variance was used to determine statistical significance except where an alternative test is specified. Statistical significance was accepted at P < 0.05.

Recruitment and Enrollment into KEEPS

Subjects were recruited through nine clinical study centers (Brigham and Women’s Hospital, Boston, MA, USA; Columbia University College of Physicians and Surgeons, New York, NY, USA; Mayo Clinic, Rochester, MN, USA; Montefiore Medical Center, Bronx, NY, USA; Kronos Longevity Research Institute, Phoenix, AZ, USA; University of California at San Francisco, San Francisco, CA, USA; University of Utah School of Medicine, Salt Lake City, UT, USA; University of Washington School of Medicine; Tacoma, WA, USA; Yale University School of Medicine, New Haven, CT, USA) beginning in July, 2005 with complete enrollment of 728 participants qualified for randomization in June, 2008. Subjects were recruited using a variety of strategies including mass mailings, recruiting posters in hospitals and clinics, print and electronic media advertisements, and an internet web page (www.keepstudy.org).

Contacts were asked a series of standard questions from a Phone Screen Interview Form. Of 4,533 phone enquiries, 2,528 callers were disqualified and 927 others, who were qualified, either failed to make or show up for screening visit appointments or did not complete screening for personal reasons. Women who qualified by phone screen gave written informed consent at their screening visit. Of these 1,078 women, 290 candidates were excluded based on history, physical examination, laboratory tests, or imaging procedures (Table 1). Some subjects had more than one disqualification factor (45 with two, seven with three, and three with four exclusions). The major cardiovascular risk factor disqualifying women was a CAC score >50 AU, which represented 3.7% of women qualified by phone screen and 14.5% of disqualified women (Table 1).

Table 1

Number of recorded reasons for disqualifications from KEEPS by screening examination(s)

	Number
History
Depression	3
Over age (>59 year)	1
LMP >3 year	6
Not menopausal	19
DES exposure in utero	3
Uterine ablation/hysterectomy	6
Cardiovascular disease	2
Medication or hormone use	14
Thromboembolic disease	2
History of cancer	2
HIV+	1
Hypertension	4
Cerebrovascular disease	1
Gall bladder disease	0
Diabetes mellitus	3
Smoke >10 cig/day	1
Other history	7
Laboratory tests
E2 >40 pg/mL	62
FSH <35 ng/mL	24
LDL >190 mg/dL	30
Trig >400 mg/dL	2
Hypothyroid (TSH >5 mL U/L)	4
Hyperthyroid (TSH <0.2, fT4 >2 mL U/L)	3
Other	15
Physical examination
Hypertension (150/95)	4
BMI >35 mm2/kg	11
Breast mass	3
Abnormal cardiovascular exama	2
Abnormal pelvic exam	5
Other PE finding	4
Other screening tests
CAC scan score >50 AU	42
CIMT scan abnormal	0
Transvaginal ultrasound >5 mm or abnormal	31
PAP smear abnormal	4
BDI score >17 (depressed)	10
MMSE score <23 (demented)	0
Mammogram abnormal	6
ECG abnormala	12
Endometrial aspirate abnormal	4
Other imaging/testing abnormal	5

LMP last menstrual period, DES diethylstilbestrol, BMI body mass index, E2 17β-estradiol, FSH follicle-stimulating hormone, LDL low-density lipoprotein, Trig triglycerides, CAC coronary arterial calcium, AU Agatston units, CIMT carotid intima–media thickness, BDI Beck Depression Inventory, MMSE minimental state examination, ECG electrocardiogram

aEvidence of myocardial infarction, angina, or congestive heart failure

The average level of education and income were greater in women meeting eligibility criteria compared to those women who were disqualified. Women who were disqualified also were more likely to have never married or declined to report marital status than women who were qualified (Table 2). Menopausal symptoms scores were similar between women excluded and those meeting eligibility criteria (9.0 ± 5.4, n = 257 and 9.4 ± 5.4, n = 728, respectively). Women meeting eligibility criteria were younger, had lower LDL cholesterol, and had lower systolic blood pressure than women excluded (Table 3).

Table 2

Demographic characteristics of women screened and qualified for KEEPS compared to those enrolled in the WHI

	KEEPS				WHI (CEE+MPA)a
	Excluded		Qualified		All average age 63 (N = 16,608)	50–59 years (N = 5,522)
	N	%	N	%	%	%
Education
Grade school	18	0.5	3	0.4	2.3	2.8
Some high school	35	0.9	3	0.4	4.5	3.9
High school or GED	166	4.4	52	7.1	19.5	16.5*
Some college/vocational	416	11.1	131	18.0	38.9	39.1*
College graduate or higher	1030	27.4	483	66.3	34.9	37.7*
Not answered	2091	55.7	56	7.7	ND	ND
Incomeb
<$10,000	48	1.3	5	0.7	5.5	6.5
$10 to <$20,000	85	2.3	10	1.4	15.0	10.2
$20 to <$ (35) 40,000	122	3.2	47	6.5	27.5	21.8
$(35) 40 to $(50) <60,000	177	4.7	77	10.6	21.2	20.6
$(50) 60 to $(75) <100,000	255	6.8	101	13.9	17.7	21.5
≥$(75) 100,000	266	7.1	122	16.8	13.2	19.4
Not answered	2803	74.6	366	50.3	ND	ND
Marital status
Married/partnered	926	24.7	479	65.8	60.1	63.6
Separated/divorced	368	9.8	134	18.4	16.8	23.6
Widowed	60	1.6	19	2.6	19.0*	7.5
Never married	946	25.2	83	11.4	4.1	5.2*
Not answered	1454	38.7	13	1.8	ND	ND
Smoking status
Never smokers	ND	ND	580	78.9	49.8	46.8*
MHT use
Never				78.7	73.4	70.5
Past				19.3	19.9	17.2
Current				1.9	7.7	12.3*
Menopausal symptoms
None	8	3.1	27	3.7	61.4	42.0*
Mild	76	29.6	203	27.9	26.2	35.6
Moderate to severe	173	67.3	498	68.4	12.4	22.4*

ND not determined or reported

*P < 0.0001 (significantly different by chi-square test from KEEPS qualified participants)

aData derived from Annals of Epidemiology 13:S78–S86, 2003 [85]

bIncome for the WHI are reported as values in parentheses

Table 3

Comparison of conventional cardiovascular risk factors of participants in KEEPS with those of WHI participants randomized to CEE+MPA

	KEEPS				WHI (CEE+MPA)a
	Excluded		Qualified
	N	Mean ± SD	N	Mean ± SD	Mean ± SD
Age	2,208	52.4 ± 3.5	728	52.7 ± 2.6***	63.3*
BMI (kg/m2)	235	26.7 ± 4.6	722	26.2 ± 4.3	28.5 ± 5.4*
Systolic BP (mm Hg)	241	122.5 ± 19.0	722	118.6 ± 15.1***	127.0 ± 7.1*
Total cholesterol (mg/dL)	237	220 ± 44	728	215 ± 29	222 ± 7*
LDL cholesterol (mg/dL)	236	134 ± 37	728	129 ± 29**	135 ± 33*
HDL cholesterol (mg/dL)	240	64 ± 18	728	65 ± 18	55 ± 14*
Triglycerides (mg/dL)	240	94 ± 53	728	90 ± 50	131 ± 59*
Fasting glucose (mg/dL)	250	88.9 ± 13.3	728	89.1 ± 9.9	98.4 ± 19.0*

aData derived from Annals of Epidemiology 13:S78–S86, 2003 [85]; for WHI—N = 16,608 for age, BMI, and systolic BP, n = 1319 for all other parameters

BMI body mass index, BP blood pressure

*P < 0.01 (statistical significance from qualified KEEPS participants); **P < 0.05 (statistical significance from women excluded from KEEPS); ***P < 0.01 (statistical significance from women excluded from KEEPS)

CIMT in women subsequently disqualified from KEEPS did not differ significantly from that of women meeting eligibility requirements [0.725 ± 0.084 mm (n = 113) and 0.726 ± 0.90 mm (n = 718), respectively]. Repeat CIMT readings on the same individual performed within 90 days had a coefficient of variation between scan 1 and scan 2 of less than 1% (0.84 ± 0.99%; minimum of 0% and a maximum of 11%).

Comparison of Demographics and Cardiovascular Risk Factors of Women Enrolled into KEEPS with Those in the WHI

Women qualified for KEEPS represent a similar ethnic mix to women enrolled in the CEE+MPA arm of the WHI (Table 4). However, women who qualified for KEEPS had higher levels of education and more declined to report their income level than women in the WHI (Table 2). The percentage of women who qualified for KEEPS who report partnered/separated and divorced status is similar to that reported in the WHI with the exception that fewer women who qualified for KEEPS reported a widowed status while more reported never having been married. This finding may reflect the difference of nearly a decade in mean ages of participants between the two studies (Tables 2 and 3). Prior hormone use did not differ between participants in KEEPS and WHI but the incidence and severity of menopausal symptoms were strikingly different, with 67% of KEEPS-qualified women reporting severe symptoms compared to 61% of WHI women reporting no symptoms. These differences were also characteristic of women in the 50–59-year grouping of the WHI with the exception of the number of participants that were current hormone users and were higher in the WHI than in women qualified for KEEPS (Table 2).

Table 4

Comparison of race/ethnicity of KEEPS participants with that of participants in WHI

Ethnicity	KEEPS cohort	WHIa
		CEE+MPA all	CEE+MPA 50–59 years
Hispanic or Latino	7.3	5.3	8.9
Caucasian not Hispanic	70.5	84.0	77.0
African American alone	7.6	6.8	9.7
American Indian and Alaskan Native	0.3	0.3	0.5
Asian/Native Hawaiian/Pacific Islander	2.6	2.2	2.4
Other race or combination	1.0	ND	ND
Unknown/not reporting	4.9	1.4	1.5

Representation of race/ethnicity as a percentage of entire cohort

ND not determined or reported

aData derived from Annals of Epidemiology 13:S78–S86, 2003 [85]

A major modifiable CVD risk factor, smoking, was also about 50% less in qualified KEEPS participants than women of the WHI. All other conventional cardiovascular risk factors comprising the Framingham Risk Score including systolic blood pressure, total cholesterol, and HDL-C were significantly different between women meeting eligibility for KEEPS and those in the WHI (Table 3). The same was true of CVD risk factors not included in the Framingham score such as BMI and fasting triglycerides and glucose, reflecting a reduced 10-year CVD risk for women in KEEPS compared to those in the WHI.

Conclusions and Future Directions

KEEPS is an example of a study that leveraged characteristics of populations in observational and epidemiological studies and incorporated design elements from basic science studies into its trial design. Thus, women who qualified for KEEPS are younger, healthier, reported more menopausal symptoms, and have fewer cardiovascular risk factors than women in the WHI. Therefore, women in KEEPS more closely match the demographics of women in observational studies. Although observational studies have been criticized for reflecting a “healthy user bias” [72], KEEPS is randomized and blinded so outcomes will not be biased by participant group assignment. Also, KEEPS is more representative of the typical clinical experience, i.e., women who are relatively healthy but who are experiencing menopausal symptoms and who seek MHT around the time of their menopausal transition.

One potential limitation of KEEPS is that the majority of participants are Caucasian and results may not be applicable to diverse ethnic populations. However, as medicine moves toward a pharmacogenomic approach to treatment choice, personal definition of ethnicity may not be as relevant as a genotypic definition. Alternatively, KEEPS may help to define the subset of women for whom MHT may provide the most favorable benefit/risk ratio. Furthermore, because a large percentage of women in KEEPS are never smokers and are otherwise at lower risk of CVD, as was the case in the Estrogen for Prevention of Atherosclerosis Trial [44], KEEPS results should more accurately reflect actions of MHT on arteries less confounded by preexisting atherosclerosis as was the case in the WHI. It might also be expected that as KEEPS proceeds, various biochemical markers and soluble cytokines known to be affected by estrogenic treatments will show less variability among participants because fewer participants are current smokers than in other studies. Smoking is well recognized as a risk factor for CVD and many of the components of tobacco smoke (particulate matter, nicotine, carbon dioxide, oxygen-derived free radicals, and other chemicals) negatively impact the same biochemical pathways affected by estrogen [5, 63, 83, 96].

In the post-WHI era, recruiting for KEEPS was challenging and the resultant socioeconomic and ethnicity demographics of qualified participants reflect difficulties in recruiting diverse groups of women. Negative reports in the lay media that accompanied the scientific reporting of results from the WHI scared many women away from use of MHT, even those who were highly symptomatic. As in observational studies where women most likely initiated MHT for menopausal symptoms, the majority of women in KEEPS reported moderate to severe menopausal symptoms, unlike the WHI in which symptomatic women were discouraged from participation. It is in women seeking treatment for symptoms that cardiovascular benefit has been reported in observational studies. However, even in the relatively asymptomatic women in the WHI, CAC scores, an outcome of KEEPS, were significantly lower in women between the ages of 50 and 59 years after 7 years of blinded randomized to CEE compared to those receiving placebo [58]. In the future, it will be important to better understand biochemical and physiological differences between women with and without menopausal symptoms and to clarify if even asymptomatic women would receive benefit from MHT for chronic diseases of aging.

An unexpected finding from the KEEPS screening processes was the number (nearly 4%) of women in this age group, usually considered at low risk for CVD that had to be excluded because of significant CAC, even in the absence of other clinically significant cardiovascular risk factors (i.e., elevated BMI and cholesterol, hypertension, and diabetes). These observations are in agreement with other studies and support the emerging concept that CAC may be a common endpoint of multiple etiologies and may be a sensitive indicator of atherosclerosis in middle-aged humans [20, 21, 26, 54, 94]. As KEEPS continues, new information will be gained regarding how MHT affects progression of CAC and if the progression is related to conventional risk factors or new biomarkers.

KEEPS will provide the first direct comparison of effects of long-term use of oral versus transdermal MHT on atherosclerosis in women. Such information is essential in order for women to select treatments which best suit their needs and lifestyle.

A major difference between KEEPS and studies in experimental animals is that KEEPS participants underwent natural menopause and not a surgical one. However, there are now established animal models of ovarian failure which should provide useful information about the similarities in vascular actions of estrogen between types of menopause [4, 45]. Furthermore, data are emerging from observational studies of women postovariectomy suggesting that MHT reduces overall mortality and maintains cardiovascular health and neurological function [3, 25, 29, 48, 51, 57, 67–70]. Prospective randomized trials are needed in this group of women to validate the effectiveness of MHT for women independent of the type of menopause [57]. In addition, more information is needed about the type, dose, and duration of progestogen therapies to reduce risk of uterine cancer but still provide cardiovascular benefit [13]. Basic science studies of the combined and pulsed use of progestogens and vascular function are scarce [2, 55, 61, 93].

KEEPS has the advantage of being able to incorporate mechanistic data from basic science studies which were not available at the time of the design of the WHI. Therefore, ancillary studies to the parent KEEPS protocol are ongoing to examine new biomarkers of disease progression and thrombotic risk [49, 50], genetic characterization of estrogen receptors relative to response to MHT and outcomes of KEEPS, measures of endothelial function, and bone health which should provide prospective data to complement data obtained from the WHI and Study of Women Across the Nation [18, 56, 78–81, 90].

Finally, there are lessons to be learned from these experiences of basic scientists and clinical investigators involved in the study of cardiovascular actions of estrogen. First, observational and epidemiological studies are hypothesis generating and do not establish cause and effect. Second, design of prospective clinical studies to test these hypotheses should mimic, as closely as possible, the study population and treatment circumstances of the observational studies. The design should also take into account data from experimental animals in which the basic information on drug action was obtained without confounding variables. Separate studies may be needed to account for such confounding variables (i.e., environmental pollutants, obesity, preexisting conditions like diabetes). Third, the design should not compromise integrity of primary outcomes for unrelated secondary outcomes. Fourth, advancements in understanding the basic mechanisms of action of sex steroids was possible in the environment of sustained funding by National Institutes of Health which attracted the best scientists to tackle difficult questions. And finally, collaboration among interdisciplinary teams will facilitate translation of information from basic science to design of clinical trials and ultimately improve the evidence upon which to base clinical practice. These lessons may be considered obvious and common sense, but are often overlooked.

The KEEPS is an example of a study that has incorporated these lessons. KEEPS has matched its sample population to that of observational studies, controlled for confounding variables such as smoking and pre-clinical vascular disease, and will evaluate a mode of treatment delivery similar to what has been used in the majority of basic science studies. Noninvasive measures of vascular health as outcomes also will allow for analysis of disease progression and help to support a paradigm shift in evaluating primary prevention [7]. Taking advantage of the latest technological advances in imaging, diagnostics, and testing, KEEPS will provide valuable information for women making decisions about MHT and their health as they age [41]. The KEEPS cohort, stored serum/plasma, and other data are valuable resources. However, additional support from funding agencies will be required in order to leverage these resources to address new questions generated by KEEPS and to translate new discoveries from basic science to further investigations of this defined cohort.