| Literature DB >> 19654009 |
Oriel M M Thekisoe1, Natasha E Rambritch, Ryo Nakao, Raoul S Bazie, Peter Mbati, Boniface Namangala, Imna Malele, Robert A Skilton, Frans Jongejan, Chihiro Sugimoto, Shin-Ichiro Kawazu, Noboru Inoue.
Abstract
We have developed two loop-mediated isothermal amplification (LAMP) assays for the detection of Theileria parva, the causative agent of East Coast fever (ECF), an economically important cattle disease in eastern, central and southern Africa. These assays target the polymorphic immunodominant molecule (PIM) and p150 LAMP genes. The primer set for each gene target consists of six primers, and each set recognises eight distinct regions on the target gene to give highly specific detection of T. parva. The detection limit of each primer set is 1fg, which is equivalent to one copy of the PIM and p150 T. parva genes. These PIM and p150 LAMP primer sets amplify DNA of T. parva isolates from cattle and buffalo from different countries including Kenya, South Africa, Tanzania, Rwanda, Uganda and Burundi, indicating their ability to detect T. parva from different countries. With the advantages of simplicity, rapidity and cost effectiveness, these LAMP assays are good candidates for molecular epidemiology studies and for monitoring control programs in ECF-endemic, resource poor countries. Copyright 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.Entities:
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Year: 2009 PMID： 19654009 DOI： 10.1016/j.ijpara.2009.07.004
Source DB: PubMed Journal: Int J Parasitol ISSN： 0020-7519 Impact factor: 3.981