| Literature DB >> 19651502 |
Sailesh Malla1, Narayan Prasad Niraula, Kwangkyoung Liou, Jae Kyung Sohng.
Abstract
The resistance genes drrABC from Streptomyces peucetius ATCC 27952 were cloned into the pIBR25 expression vector under a strong ermE* promoter to enhance doxorubicin (DXR) production. The recombinant expression plasmids, pDrrAB25, pDrrC25 and pDrrABC25, were constructed to overexpress drrAB, drrC and drrABC, respectively, in S. peucetius ATCC 27952. The recombinant strains produced more DXR than the parental strain: a 2.2-fold increase with pDrrAB25, a 5.1-fold increase with pDrrC25, and a 2.4-fold increase with pDrrABC25. We also studied the relative ratios of doxorubicin, daunorubicin and epsilon-rhodomycinone produced in these recombinant strains. Copyright 2009 Elsevier GmbH. All rights reserved.Entities:
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Year: 2009 PMID： 19651502 DOI： 10.1016/j.micres.2009.04.002
Source DB: PubMed Journal: Microbiol Res ISSN： 0944-5013 Impact factor: 5.415