Literature DB >> 19648917

Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms.

Jonas Ries1, Shuizi Rachel Yu, Markus Burkhardt, Michael Brand, Petra Schwille.   

Abstract

Analysis of receptor-ligand interactions in vivo is key to biology but poses a considerable challenge to quantitative microscopy. Here we combine static-volume, two-focus and dual-color scanning fluorescence correlation spectroscopy to solve this task at cellular resolution in complex biological environments. We quantified the mobility of fibroblast growth factor receptors Fgfr1 and Fgfr4 in cell membranes of living zebrafish embryos and determined their in vivo binding affinities to their ligand Fgf8.

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Year:  2009        PMID： 19648917     DOI： 10.1038/nmeth.1355

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  16 in total

1.  Endocytosis controls spreading and effective signaling range of Fgf8 protein.

Authors:  Steffen Scholpp; Michael Brand
Journal:  Curr Biol       Date:  2004-10-26       Impact factor: 10.834

2.  Receptor-ligand binding assays: technologies and applications.

Authors:  Lutea A A de Jong; Donald R A Uges; Jan Piet Franke; Rainer Bischoff
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2005-10-25       Impact factor: 3.205

3.  Studying slow membrane dynamics with continuous wave scanning fluorescence correlation spectroscopy.

Authors:  Jonas Ries; Petra Schwille
Journal:  Biophys J       Date:  2006-06-16       Impact factor: 4.033

4.  Fluorescence cross-correlation spectroscopy in living cells.

Authors:  Kirsten Bacia; Sally A Kim; Petra Schwille
Journal:  Nat Methods       Date:  2006-02       Impact factor: 28.547

5.  Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling.

Authors:  Celine I Maeder; Mark A Hink; Ali Kinkhabwala; Reinhard Mayr; Philippe I H Bastiaens; Michael Knop
Journal:  Nat Cell Biol       Date:  2007-10-21       Impact factor: 28.824

6.  New concepts for fluorescence correlation spectroscopy on membranes.

Authors:  Jonas Ries; Petra Schwille
Journal:  Phys Chem Chem Phys       Date:  2008-03-27       Impact factor: 3.676

7.  Fast multi-tau real-time software correlator for dynamic light scattering.

Authors:  D Magatti; F Ferri
Journal:  Appl Opt       Date:  2001-08-20       Impact factor: 1.980

8.  fgfr-1 is required for embryonic growth and mesodermal patterning during mouse gastrulation.

Authors:  T P Yamaguchi; K Harpal; M Henkemeyer; J Rossant
Journal:  Genes Dev       Date:  1994-12-15       Impact factor: 11.361

9.  Murine FGFR-1 is required for early postimplantation growth and axial organization.

Authors:  C X Deng; A Wynshaw-Boris; M M Shen; C Daugherty; D M Ornitz; P Leder
Journal:  Genes Dev       Date:  1994-12-15       Impact factor: 11.361

10.  FGFR-3 and FGFR-4 function cooperatively to direct alveogenesis in the murine lung.

Authors:  M Weinstein; X Xu; K Ohyama; C X Deng
Journal:  Development       Date:  1998-09       Impact factor: 6.868
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  49 in total

1.  Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy.

Authors:  Yong Hwee Foo; Nikolaus Naredi-Rainer; Don C Lamb; Sohail Ahmed; Thorsten Wohland
Journal:  Biophys J       Date:  2012-03-06       Impact factor: 4.033

Review 2.  Proteins on the move: insights gained from fluorescent protein technologies.

Authors:  Atsushi Miyawaki
Journal:  Nat Rev Mol Cell Biol       Date:  2011-09-23       Impact factor: 94.444

3.  Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules.

Authors:  Shuizi Rachel Yu; Markus Burkhardt; Matthias Nowak; Jonas Ries; Zdenek Petrásek; Steffen Scholpp; Petra Schwille; Michael Brand
Journal:  Nature       Date:  2009-09-09       Impact factor: 49.962

Review 4.  Fluorescence techniques to study lipid dynamics.

Authors:  Erdinc Sezgin; Petra Schwille
Journal:  Cold Spring Harb Perspect Biol       Date:  2011-11-01       Impact factor: 10.005

5.  Probing transcription factor diffusion dynamics in the living mammalian embryo with photoactivatable fluorescence correlation spectroscopy.

Authors:  Gurpreet Kaur; Mauro W Costa; Christian M Nefzger; Juan Silva; Juan Carlos Fierro-González; Jose M Polo; Toby D M Bell; Nicolas Plachta
Journal:  Nat Commun       Date:  2013       Impact factor: 14.919

6.  Self-segregation of myelin membrane lipids in model membranes.

Authors:  Larisa Yurlova; Nicoletta Kahya; Shweta Aggarwal; Hermann-Josef Kaiser; Salvatore Chiantia; Mostafa Bakhti; Yael Pewzner-Jung; Oshrit Ben-David; Anthony H Futerman; Britta Brügger; Mikael Simons
Journal:  Biophys J       Date:  2011-12-07       Impact factor: 4.033

7.  Endocytosis and signaling during development.

Authors:  Christian Bökel; Michael Brand
Journal:  Cold Spring Harb Perspect Biol       Date:  2014-03-01       Impact factor: 10.005

Review 8.  Quantifying intracellular dynamics using fluorescence fluctuation spectroscopy.

Authors:  Mark A Hink
Journal:  Protoplasma       Date:  2014-01-14       Impact factor: 3.356

9.  Splitting the Difference: Sorting Photons to Improve Quantitative Measurements in Correlation Spectroscopy.

Authors:  Thorsten Wohland
Journal:  Biophys J       Date:  2020-08-20       Impact factor: 4.033

Review 10.  Recent developments in fluorescence correlation spectroscopy for diffusion measurements in planar lipid membranes.

Authors:  Radek Macháň; Martin Hof
Journal:  Int J Mol Sci       Date:  2010-01-28       Impact factor: 6.208
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