Literature DB >> 19647745

Multiplexed labeling of samples with cell tracking dyes facilitates rapid and accurate internally controlled calcium flux measurement by flow cytometry.

Guo Fu1, Nicholas R J Gascoigne.   

Abstract

Calcium flux measurement is a crucial assay in lymphocyte activation. However, with the currently well established flow cytometric methods, it is a tedious procedure that is difficult to control to avoid variation between samples. This leads to unwanted sources of error that can make it problematic to interpret the results. Here we present an improved method that allows different cell populations to be tested in the same sample. Samples are pre-labeled with CFSE or Cy5 then mixed and stimulated to induce calcium flux. This facilitates more rapid and accurate measurement of calcium flux and also dramatically reduces the cost and effort required for this type of assay.

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Year:  2009        PMID： 19647745      PMCID： PMC2782925          DOI： 10.1016/j.jim.2009.07.009

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  Tracking antigen-specific lymphocytes in vivo.

Authors:  Claire L Adams; Catherine M Rush; Karen M Smith; Paul Garside
Journal:  Methods Mol Biol       Date:  2004

Review 2.  Fluorescent measurement of [Ca2+]c: basic practical considerations.

Authors:  Alec W M Simpson
Journal:  Methods Mol Biol       Date:  2006

3.  Measurement of [Ca2+] in cell suspensions using indo-1.

Authors:  Adriaan Nelemans
Journal:  Methods Mol Biol       Date:  2006

4.  Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester.

Authors:  Ben J C Quah; Hilary S Warren; Christopher R Parish
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

5.  Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data.

Authors:  Edwin D Hawkins; Mirja Hommel; Marian L Turner; Francis L Battye; John F Markham; Philip D Hodgkin
Journal:  Nat Protoc       Date:  2007       Impact factor: 13.491

6.  Altered T cell receptor signaling and disrupted T cell development in mice lacking Itk.

Authors:  X C Liao; D R Littman
Journal:  Immunity       Date:  1995-12       Impact factor: 31.745

7.  Themis controls thymocyte selection through regulation of T cell antigen receptor-mediated signaling.

Authors:  Guo Fu; Sébastien Vallée; Vasily Rybakin; Marielena V McGuire; Jeanette Ampudia; Claudia Brockmeyer; Mogjiborahman Salek; Paul R Fallen; John A H Hoerter; Anil Munshi; Yina H Huang; Jianfang Hu; Howard S Fox; Karsten Sauer; Oreste Acuto; Nicholas R J Gascoigne
Journal:  Nat Immunol       Date:  2009-07-13       Impact factor: 25.606

Review 8.  Calcium signalling in lymphocyte activation and disease.

Authors:  Stefan Feske
Journal:  Nat Rev Immunol       Date:  2007-08-17       Impact factor: 53.106

9.  The lupus-related Lmb3 locus contains a disease-suppressing Coronin-1A gene mutation.

Authors:  M Katarina Haraldsson; Christine A Louis-Dit-Sully; Brian R Lawson; Gabriel Sternik; Marie-Laure Santiago-Raber; Nicholas R J Gascoigne; Argyrios N Theofilopoulos; Dwight H Kono
Journal:  Immunity       Date:  2008-01       Impact factor: 31.745

10.  T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells.

Authors:  K Q Liu; S C Bunnell; C B Gurniak; L J Berg
Journal:  J Exp Med       Date:  1998-05-18       Impact factor: 14.307
  10 in total
  11 in total

1.  T cell receptor and cytokine signal integration in CD8+ T cells is mediated by the protein Themis.

Authors:  Joanna Brzostek; Namrata Gautam; Xiang Zhao; Elijah W Chen; Monika Mehta; Desmond W H Tung; Yen Leong Chua; Jiawei Yap; Su H Cho; Shvetha Sankaran; Vasily Rybakin; Guo Fu; Nicholas R J Gascoigne
Journal:  Nat Immunol       Date:  2020-01-13       Impact factor: 25.606

2.  Protein kinase C η is required for T cell activation and homeostatic proliferation.

Authors:  Guo Fu; Jianfang Hu; Nathalie Niederberger-Magnenat; Vasily Rybakin; Javier Casas; Pia P Yachi; Stephanie Feldstein; Bo Ma; John A H Hoerter; Jeanette Ampudia; Stephanie Rigaud; Florence Lambolez; Amanda L Gavin; Karsten Sauer; Hilde Cheroutre; Nicholas R J Gascoigne
Journal:  Sci Signal       Date:  2011-12-06       Impact factor: 8.192

3.  Divergent requirement for Gαs and cAMP in the differentiation and inflammatory profile of distinct mouse Th subsets.

Authors:  Xiangli Li; Fiona Murray; Naoki Koide; Jonathan Goldstone; Sara M Dann; Jianzhong Chen; Samuel Bertin; Guo Fu; Lee S Weinstein; Min Chen; Maripat Corr; Lars Eckmann; Paul A Insel; Eyal Raz
Journal:  J Clin Invest       Date:  2012-02-13       Impact factor: 14.808

4.  The Bacterial Peptidoglycan-Sensing Molecules NOD1 and NOD2 Promote CD8+ Thymocyte Selection.

Authors:  Marianne M Martinic; Irina Caminschi; Meredith O'Keeffe; Therese C Thinnes; Raelene Grumont; Steve Gerondakis; Dianne B McKay; David Nemazee; Amanda L Gavin
Journal:  J Immunol       Date:  2017-02-15       Impact factor: 5.422

5.  In vivo, multimodal imaging of B cell distribution and response to antibody immunotherapy in mice.

Authors:  Daniel L J Thorek; Patricia Y Tsao; Vaishali Arora; Lanlan Zhou; Robert A Eisenberg; Andrew Tsourkas
Journal:  PLoS One       Date:  2010-05-17       Impact factor: 3.240

6.  Noncatalytic Bruton's tyrosine kinase activates PLCγ2 variants mediating ibrutinib resistance in human chronic lymphocytic leukemia cells.

Authors:  Martin Wist; Laura Meier; Orit Gutman; Jennifer Haas; Sascha Endres; Yuan Zhou; Reinhild Rösler; Sebastian Wiese; Stephan Stilgenbauer; Elias Hobeika; Yoav I Henis; Peter Gierschik; Claudia Walliser
Journal:  J Biol Chem       Date:  2020-03-17       Impact factor: 5.157

7.  The ion channel TRPV1 regulates the activation and proinflammatory properties of CD4⁺ T cells.

Authors:  Lilian L Nohara; Hongjian Xu; Samuel Bertin; Yukari Aoki-Nonaka; Petrus Rudolf de Jong; Shawna R Stanwood; Sonal Srikanth; Jihyung Lee; Keith To; Lior Abramson; Timothy Yu; Tiffany Han; Ranim Touma; Xiangli Li; José M González-Navajas; Scott Herdman; Maripat Corr; Guo Fu; Hui Dong; Yousang Gwack; Alessandra Franco; Wilfred A Jefferies; Eyal Raz
Journal:  Nat Immunol       Date:  2014-10-05       Impact factor: 25.606

8.  Negative selection assay based on stimulation of T cell receptor transgenic thymocytes with peptide-MHC tetramers.

Authors:  Vasily Rybakin; Nicholas R J Gascoigne
Journal:  PLoS One       Date:  2012-08-10       Impact factor: 3.240

9.  Calcium signals regulate the functional differentiation of thymic iNKT cells.

Authors:  Meng Zhao; Ariel Quintana; Chen Zhang; Alexander Y Andreyev; William Kiosses; Tomomi Kuwana; Anne Murphy; Patrick G Hogan; Mitchell Kronenberg
Journal:  EMBO J       Date:  2021-06-25       Impact factor: 14.012

10.  Themis sets the signal threshold for positive and negative selection in T-cell development.

Authors:  Guo Fu; Javier Casas; Stephanie Rigaud; Vasily Rybakin; Florence Lambolez; Joanna Brzostek; John A H Hoerter; Wolfgang Paster; Oreste Acuto; Hilde Cheroutre; Karsten Sauer; Nicholas R J Gascoigne
Journal:  Nature       Date:  2013-11-13       Impact factor: 49.962
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