Literature DB >> 1964477

Diagnosis of herpes encephalitis via Southern blotting of cerebrospinal fluid DNA amplified by polymerase chain reaction.

P E Klapper1, G M Cleator, C Dennett, A G Lewis.   

Abstract

Herpes simplex virus thymidine kinase gene specific polymerase chain reaction (PCR) amplification of DNA extracted from lumbar cerebrospinal fluid (CSF) and Southern blotting (SB) were evaluated as a method for the diagnosis of herpes simplex encephalitis (HSE). Positive PCR-SB results were obtained with CSF samples from 9 of 10 patients (11 of 12 CSF specimens) with proven herpes encephalitis as early as 2 days after onset of neurological illness. Our data support the suggestion that PCR techniques may provide a clinically relevant "non-invasive" method for the diagnosis of HSE.

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Year:  1990        PMID： 1964477     DOI： 10.1002/jmv.1890320413

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  18 in total

Review 1.  Molecular diagnosis of herpes simplex virus infections in the central nervous system.

Authors:  Y W Tang; P S Mitchell; M J Espy; T F Smith; D H Persing
Journal:  J Clin Microbiol       Date:  1999-07       Impact factor: 5.948

2.  Detection of Herpes simplex virus DNA by real-time PCR.

Authors:  H H Kessler; G Mühlbauer; B Rinner; E Stelzl; A Berger; H W Dörr; B Santner; E Marth; H Rabenau
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

3.  Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid.

Authors:  V Hukkanen; T Rehn; R Kajander; M Sjöroos; M Waris
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

4.  Genotyping of herpes simplex viruses by polymerase chain reaction.

Authors:  H Piiparinen; A Vaheri
Journal:  Arch Virol       Date:  1991       Impact factor: 2.574

5.  Amplification and characterization of herpesvirus DNA in cerebrospinal fluid from patients with acute encephalitis.

Authors:  F Rozenberg; P Lebon
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

6.  Unusual course of herpes simplex virus encephalitis after acyclovir therapy.

Authors:  W Preiser; B Weber; G Klös; P A Fischer; H W Doerr
Journal:  Infection       Date:  1996 Sep-Oct       Impact factor: 3.553

7.  Nested PCR for rapid detection of mumps virus in cerebrospinal fluid from patients with neurological diseases.

Authors:  G P Poggio; C Rodriguez; D Cisterna; M C Freire; J Cello
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

Review 8.  The role of laboratory investigation in the diagnosis and management of patients with suspected herpes simplex encephalitis: a consensus report. The EU Concerted Action on Virus Meningitis and Encephalitis.

Authors:  P Cinque; G M Cleator; T Weber; P Monteyne; C J Sindic; A M van Loon
Journal:  J Neurol Neurosurg Psychiatry       Date:  1996-10       Impact factor: 10.154

9.  Comparative evaluation of colorimetric microtiter plate systems for detection of herpes simplex virus in cerebrospinal fluid.

Authors:  Y W Tang; P N Rys; B J Rutledge; P S Mitchell; T F Smith; D H Persing
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

10.  Measles encephalitis during immunosuppressive treatment for acute lymphoblastic leukaemia.

Authors:  I Hughes; M E Jenney; R W Newton; D J Morris; P E Klapper
Journal:  Arch Dis Child       Date:  1993-06       Impact factor: 3.791
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