Literature DB >> 19639147

Exchangeable oxygens in the vicinity of the molybdenum center of the high-pH form of sulfite oxidase and sulfite dehydrogenase.

Andrei V Astashkin1, Eric L Klein, Dmitry Ganyushin, Kayunta Johnson-Winters, Frank Neese, Ulrike Kappler, John H Enemark.   

Abstract

The electron spin echo envelope modulation (ESEEM) investigation of the high-pH (hpH) form of sulfite oxidase (SO) and sulfite dehydrogenase (SDH) prepared in buffer enriched with H(2)(17)O reveals the presence of three types of exchangeable oxygen atoms at the molybdenum center. Two of these oxygen atoms belong to the equatorial OH ligand and the axial oxo ligand, and are characterized by (17)O hyperfine interaction (hfi) constants of about 37 MHz and 6 MHz, respectively. The third oxygen has an isotropic hfi constant of 3-4 MHz and likely belongs to a hydroxyl moiety hydrogen-bonded to the equatorial OH ligand. This exchangeable oxygen atom is not observed in the ESEEM spectra of the Y236F mutant of SDH, where the active site tyrosine has been replaced by phenylalanine.

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Year:  2009        PMID： 19639147      PMCID： PMC2789977          DOI： 10.1039/b907029j

Source DB:  PubMed          Journal:  Phys Chem Chem Phys        ISSN： 1463-9076            Impact factor:   3.676


  34 in total

1.  Pulsed EPR studies of the exchangeable proton at the molybdenum center of dimethyl sulfoxide reductase.

Authors:  Arnold M Raitsimring; Andrei V Astashkin; Changjian Feng; John H Enemark; Kimberly Johnson Nelson; K V Rajagopalan
Journal:  J Biol Inorg Chem       Date:  2002-08-30       Impact factor: 3.358

2.  Comparison of the molybdenum centres of native and desulpho xanthine oxidase. The nature of the cyanide-labile sulphur atom and the nature of the proton-accepting group.

Authors:  S Gutteridge; S J Tanner; R C Bray
Journal:  Biochem J       Date:  1978-12-01       Impact factor: 3.857

3.  Calculation of electric-field gradients based on higher-order generalized Douglas-Kroll transformations.

Authors:  Frank Neese; Alexander Wolf; Timo Fleig; Markus Reiher; Bernd Artur Hess
Journal:  J Chem Phys       Date:  2005-05-22       Impact factor: 3.488

4.  17O ESEEM evidence for exchange of the axial oxo ligand in the molybdenum center of the high pH form of sulfite oxidase.

Authors:  Andrei V Astashkin; Changjian Feng; Arnold M Raitsimring; John H Enemark
Journal:  J Am Chem Soc       Date:  2005-01-19       Impact factor: 15.419

5.  Purification and properties of sulfite oxidase from chicken liver. Presence of molybdenum in sulfite oxidase from diverse sources.

Authors:  D L Kessler; K V Rajagopalan
Journal:  J Biol Chem       Date:  1972-10-25       Impact factor: 5.157

6.  Identification and biochemical characterization of Arabidopsis thaliana sulfite oxidase. A new player in plant sulfur metabolism.

Authors:  T Eilers; G Schwarz; H Brinkmann; C Witt; T Richter; J Nieder; B Koch; R Hille; R Hänsch; R R Mendel
Journal:  J Biol Chem       Date:  2001-10-11       Impact factor: 5.157

7.  Pulsed EPR studies of nonexchangeable protons near the Mo(V) center of sulfite oxidase: direct detection of the alpha-proton of the coordinated cysteinyl residue and structural implications for the active site.

Authors:  Andrei V Astashkin; Arnold M Raitsimring; Changjian Feng; Jean L Johnson; K V Rajagopalan; John H Enemark
Journal:  J Am Chem Soc       Date:  2002-05-29       Impact factor: 15.419

8.  Structural studies of the molybdenum center of the pathogenic R160Q mutant of human sulfite oxidase by pulsed EPR spectroscopy and 17O and 33S labeling.

Authors:  Andrei V Astashkin; Kayunta Johnson-Winters; Eric L Klein; Changjian Feng; Heather L Wilson; K V Rajagopalan; Arnold M Raitsimring; John H Enemark
Journal:  J Am Chem Soc       Date:  2008-06-05       Impact factor: 15.419

9.  Intramolecular electron transfer in sulfite-oxidizing enzymes: elucidating the role of a conserved active site arginine.

Authors:  Safia Emesh; Trevor D Rapson; Asha Rajapakshe; Ulrike Kappler; Paul V Bernhardt; Gordon Tollin; John H Enemark
Journal:  Biochemistry       Date:  2009-03-17       Impact factor: 3.162

10.  Equilibria amongst different molybdenum (V)-containing species from sulphite oxidase. Evidence for a halide ligand of molybdenum in the low-pH species.

Authors:  R C Bray; S Gutteridge; M T Lamy; T Wilkinson
Journal:  Biochem J       Date:  1983-04-01       Impact factor: 3.857
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  11 in total

1.  Applications of pulsed EPR spectroscopy to structural studies of sulfite oxidizing enzymes().

Authors:  Eric L Klein; Andrei V Astashkin; Arnold M Raitsimring; John H Enemark
Journal:  Coord Chem Rev       Date:  2013-01-01       Impact factor: 22.315

Review 2.  The mononuclear molybdenum enzymes.

Authors:  Russ Hille; James Hall; Partha Basu
Journal:  Chem Rev       Date:  2014-01-28       Impact factor: 60.622

3.  Intramolecular electron transfer in sulfite-oxidizing enzymes: probing the role of aromatic amino acids.

Authors:  Asha Rajapakshe; Kimberly T Meyers; Robert E Berry; Gordon Tollin; John H Enemark
Journal:  J Biol Inorg Chem       Date:  2011-11-05       Impact factor: 3.358

4.  Structural studies of the molybdenum center of mitochondrial amidoxime reducing component (mARC) by pulsed EPR spectroscopy and 17O-labeling.

Authors:  Asha Rajapakshe; Andrei V Astashkin; Eric L Klein; Debora Reichmann; Ralf R Mendel; Florian Bittner; John H Enemark
Journal:  Biochemistry       Date:  2011-09-22       Impact factor: 3.162

5.  Identity of the exchangeable sulfur-containing ligand at the Mo(V) center of R160Q human sulfite oxidase.

Authors:  Eric L Klein; Arnold M Raitsimring; Andrei V Astashkin; Asha Rajapakshe; Kayunta Johnson-Winters; Anna R Arnold; Alexey Potapov; Daniella Goldfarb; John H Enemark
Journal:  Inorg Chem       Date:  2012-01-06       Impact factor: 5.165

6.  Implications for the mechanism of sulfite oxidizing enzymes from pulsed EPR spectroscopy and DFT calculations for "difficult" nuclei.

Authors:  John H Enemark; Arnold M Raitsimring; Andrei V Astashkin; Eric L Klein
Journal:  Faraday Discuss       Date:  2011       Impact factor: 4.008

7.  Characterization of chloride-depleted human sulfite oxidase by electron paramagnetic resonance spectroscopy: experimental evidence for the role of anions in product release.

Authors:  Asha Rajapakshe; Kayunta Johnson-Winters; Anna R Nordstrom; Kimberly T Meyers; Safia Emesh; Andrei V Astashkin; John H Enemark
Journal:  Biochemistry       Date:  2010-06-29       Impact factor: 3.162

Review 8.  Elucidating the catalytic mechanism of sulfite oxidizing enzymes using structural, spectroscopic, and kinetic analyses.

Authors:  Kayunta Johnson-Winters; Gordon Tollin; John H Enemark
Journal:  Biochemistry       Date:  2010-08-31       Impact factor: 3.162

9.  Pulsed EPR investigations of the Mo(V) centers of the R55Q and R55M variants of sulfite dehydrogenase from Starkeya novella.

Authors:  Trevor D Rapson; Andrei V Astashkin; Kayunta Johnson-Winters; Paul V Bernhardt; Ulrike Kappler; Arnold M Raitsimring; John H Enemark
Journal:  J Biol Inorg Chem       Date:  2010-01-19       Impact factor: 3.358

10.  Molybdenum and Tungsten Cofactors and the Reactions They Catalyze.

Authors:  Martin L Kirk; Khadanand Kc
Journal:  Met Ions Life Sci       Date:  2020-03-23
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